Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter

<p>Abstract</p> <p>Background</p> <p>Purifying stem cells is an inevitable process for further investigation and cell-therapy. Sorting side population (SP) cells is generally regarded as an effective method to enrich for progenitor cells. This study was to explore whether sorting SP could enrich for the Musashi1 (Msi1) positive cells from Msi1 high expression cells (Msi1^highcells) derived from mouse embryonic stem cells (ESCs) in vitro.</p> <p>Results</p> <p>In this study, Msi1^highcell population derived from ESCs were stained by Hoechst 33342, and then the SP and non-SP (NSP) fractions were analyzed and sorted by fluorescence activated cell sorter. Subsequently, the expressions of Msi1 and other markers for neural and intestinal stem cells in SP and NSP were respectively detected. SP and NSP cells were hypodermically engrafted into the backs of NOD/SCID mice to form grafts. The developments of neural and intestinal epithelial cells in these grafts were investigated. SP fraction was identified and isolated from Msi1^highcell population. The expression of Msi1 in SP fraction was significantly higher than that in NSP fraction and unsorted Msi1^highcells (<it>P</it>< 0.05). Furthermore, the markers for neural cells and intestinal epithelial cells were more highly expressed in the grafts from SP fraction than those from NSP fraction (<it>P</it>< 0.05).</p> <p>Conclusions</p> <p>SP fraction, isolated from Msi1^highcells, contains almost all the Msi1-positive cells and has the potential to differentiate into neural and intestinal epithelial cells in vivo. Sorting SP fraction could be a convenient and practical method to enrich for Msi1-positive cells from the differentiated cell population derived from ESCs.</p

Observation of the Singly Cabibbo-Suppressed Decay Λc+→Σ−K+π+\Lambda_{c}^{+}\to \Sigma^{-}K^{+}\pi^{+}

The singly Cabibbo-suppressed decay $\Lambda_{c}^{+}\to \Sigma^{-}K^{+}\pi^{+}$ is observed for the first time with a statistical significance of $6.4\sigma$ by using 4.5 fb$^{-1}$ of $e^+e^-$ collision data collected at center-of-mass energies between 4.600 and 4.699 GeV with the BESIII detector at BEPCII. The absolute branching fraction of $\Lambda_{c}^{+}\to \Sigma^{-}K^{+}\pi^{+}$ is measured to be $(3.8\pm1.3_{\rm stat}\pm0.2_{\rm syst})\times 10^{-4}$ in a model-independent approach. This is the first observation of a Cabibbo-suppressed $\Lambda_{c}^{+}$ decay involving $\Sigma^-$ in the final state. The ratio of branching fractions between $\Lambda_{c}^{+}\to \Sigma^{-}K^{+}\pi^{+}$ and the Cabibbo-favored decay $\Lambda_{c}^{+}\to \Sigma^- \pi^+\pi^+$ is calculated to be $(0.4 \pm 0.1)s_{c}^{2}$, where $s_{c} \equiv \sin\theta_c = 0.2248$ with $\theta_c$ the Cabibbo mixing angle. This ratio significantly deviates from $1.0s_{c}^{2}$ and provides important information for the understanding of nonfactorization contributions in $\Lambda_{c}^{+}$ decays.Comment: 8 pages, 2 figure

Updated measurements of the M1 transition ψ(3686)→γηc(2S)\psi(3686) \to \gamma \eta_{c}(2S) with ηc(2S)→KKˉπ\eta_{c}(2S) \to K \bar{K} \pi

Based on a data sample of $(27.08 \pm 0.14 ) \times 10^8~\psi(3686)$ events collected with the BESIII detector at the BEPCII collider, the M1 transition $\psi(3686) \to \gamma \eta_{c}(2S)$ with $\eta_{c}(2S) \to K\bar{K}\pi$ is studied, where $K\bar{K}\pi$ is $K^{+} K^{-} \pi^{0}$ or $K_{S}^{0}K^{\pm}\pi^{\mp}$. The mass and width of the $\eta_{c}(2S)$ are measured to be $(3637.8 \pm 0.8 (\rm {stat}) \pm 0.2 (\rm {syst}))$ MeV/$c^{2}$ and $(10.5 \pm 1.7 (\rm {stat}) \pm 3.5 (\rm {syst}))$ MeV, respectively. The product branching fraction $\mathcal{B}\left(\psi(3686) \rightarrow \gamma \eta_{c}(2 S)\right) \times \mathcal{B}(\eta_{c}(2 S) \rightarrow K \bar{K} \pi)$ is determined to be $(0.97 \pm 0.06 (\rm {stat}) \pm 0.09 (\rm {syst})) \times 10^{-5}$. Using $\mathcal{BR}(\eta_{c}(2S)\to K\bar{K}\pi)=(1.86^{+0.68}_{-0.49})\%$, we obtain the branching fraction of the radiative transition to be $\mathcal{BR}(\psi(3686) \to \gamma \eta_{c}(2S)) = (5.2 \pm 0.3 (\rm {stat}) \pm 0.5 (\rm {syst}) ^{+1.9}_{-1.4} (extr)) \times 10^{-4}$, where the third uncertainty is due to the quoted $\mathcal{BR}(\eta_{c}(2S) \to K\bar{K}\pi)$