Femtomolar detection of the heart failure biomarker NT-proBNP in artificial saliva using an immersible liquid-gated aptasensor with reduced graphene oxide

Measuring NT-proBNP biomarker is recommended for preliminary diagnostics of the heart failure. Recent studies suggest a possibility of early screening of biomarkers in saliva for non-invasive identification of cardiac diseases at the point-of-care. However, NT-proBNP concentrations in saliva can be thousand time lower than in blood plasma, going down to pg/mL level. To reach this level, we developed a label-free aptasensor based on a liquid-gated field effect transistor using a film of reduced graphene oxide monolayer (rGO-FET) with immobilized NT-proBNP specific aptamer. We found that, depending on ionic strength of tested solutions, there were different levels of correlation in responses of electrical parameters of the rGO-FET aptasensor, namely, the Dirac point shift and transconductance change. The correlation in response to NT-proBNP was high for 1.6 mM phosphate-buffered saline (PBS) and zero for 16 mM PBS in a wide range of analyte concentrations, varied from 1 fg/mL to 10 ng/mL. The effects of transconductance and Dirac point shift in PBS solutions of different concentrations are discussed. The biosensor exhibited a high sensitivity for both transconductance (2 uS/decade) and Dirac point shift (2.3 mV/decade) in diluted PBS with the linear range from 10 fg/mL to 1 pg/mL. The aptasensor performance has been also demonstrated in undiluted artificial saliva with the achieved limit of detection down to 41 fg/mL (~4.6 fM)

Common origin of quasi-periodic pulsations in microwave and decimetric solar radio bursts

We analyse quasi-periodic pulsations (QPP) detected in the microwave and decimetre radio emission of the 5 May 2017 7:04 UT (SOL2017-09-05T07:04) solar flare, using simultaneous observations by the Siberian Radioheliograph 48 (SRH-48, 4 – 8 GHz) and Mingantu Spectral Radioheliograph (MUSER-I, 0.4 – 2 GHz). The microwave emission was broadband with a typical gyrosynchrotron spectrum, while a quasi-periodic enhancement of the decimetric emission appeared in a narrow spectral band (500 – 700 MHz), consistent with the coherent-plasma-emission mechanism. The periodicity that we found in microwaves is about 30 seconds, coming from a compact loop-like source with a typical height of about 31 Mm. The decimetric emission exhibited a periodicity of about 6 seconds. We suggest a qualitative scenario linking the QPPs observed in both incoherent and coherent spectral bands and their generation mechanisms. The properties of the QPPs found in the microwave signal are typical for perturbations of the flare loop by the standing sausage mode of a fast magnetohydrodynamic (MHD) wave. Our analysis indicated that this sausage-oscillating flare loop was the primary source of oscillations in the discussed event. The suggested scenario is that a fundamental sausage harmonic is the dominant cause for the observed QPPs in the microwave emission. The initiation of oscillations in the decimetric emission is caused by the third sausage harmonic via periodic and nonlinear triggering of the acceleration processes in the current sheets, formed at the interface between the sausage-oscillating flare loop and the external coronal loop that extended to higher altitudes. Our results demonstrate the possible role of MHD wave processes in the release and transport of energy during solar flares, linking coherent and incoherent radio emission mechanisms

Comparative Study of Field-Effect Transistors Based on Graphene Oxide and CVD Graphene in Highly Sensitive NT-proBNP Aptasensors

Graphene-based materials are actively being investigated as sensing elements for the detection of different analytes. Both graphene grown by chemical vapor deposition (CVD) and graphene oxide (GO) produced by the modified Hummers’ method are actively used in the development of biosensors. The production costs of CVD graphene- and GO-based sensors are similar; however, the question remains regarding the most efficient graphene-based material for the construction of point-of-care diagnostic devices. To this end, in this work, we compare CVD graphene aptasensors with the aptasensors based on reduced GO (rGO) for their capabilities in the detection of NT-proBNP, which serves as the gold standard biomarker for heart failure. Both types of aptasensors were developed using commercial gold interdigitated electrodes (IDEs) with either CVD graphene or GO formed on top as a channel of liquid-gated field-effect transistor (FET), yielding GFET and rGO-FET sensors, respectively. The functional properties of the two types of aptasensors were compared. Both demonstrate good dynamic range from 10 fg/mL to 100 pg/mL. The limit of detection for NT-proBNP in artificial saliva was 100 fg/mL and 1 pg/mL for rGO-FET- and GFET-based aptasensors, respectively. While CVD GFET demonstrates less variations in parameters, higher sensitivity was demonstrated by the rGO-FET due to its higher roughness and larger bandgap. The demonstrated low cost and scalability of technology for both types of graphene-based aptasensors may be applicable for the development of different graphene-based biosensors for rapid, stable, on-site, and highly sensitive detection of diverse biochemical markers