The efficacy of using ozone in dentistry – review

The aim of this article was to present the properties of ozone and its use in dentistry. Ozone is a chemical compound consisting of three atoms of oxygen with the molecular weight of 47.98 g/mol. It is an energy-rich and highly unstable form of oxygen. Ozone is a powerful oxidizer and it is strongly effective against bacteria, fungi, viruses and parasites. It has been used successfully in the field of medicine for more than a decade. There are several known actions of ozone on the human body, such as: immunostimulating and analgesic, antimicrobial, bioenergetic and biosynthetic. Based on its properties, ozone is being used in various fields in dentistry, such as caries prevention, endodontics, periodontology, prosthetics, orthodontics and oral surgery. Modern dentistry is moving towards the use of minimally invasive procedures. Ozone therapy is quite promising as it is less invasive, has a potent disinfectant property and has minimal adverse effects. Evidence based studies and clinical trials are still required.Celem niniejszej pracy jest zaprezentowanie właściwości, mechanizmu działania oraz skuteczności zastosowania ozonu we współczesnej stomatologii. Ozon jako alotropowa forma tlenu, o wzorze chemicznym O3, ma silne właściwości utleniające, co sprawia, że na jego działanie podatne są bakterie Gram-dodatnie, Gram-ujemne, wirusy, spory oraz komórki wegetatywne. Szerokie zastosowanie terapeutyczne w stomatologii ozon zawdzięcza właściwościom: przeciwdrobnoustrojowym, stymulującym układ odpornościowy, przeciwbólowym, detoksykacyjnym, antyhipoksyjnym, biosyntetycznym oraz biostymulującym. Ponadto znajduje zastosowanie w wielu dziedzinach stomatologii: w profilaktyce, stomatologii zachowawczej (wybielanie zębów, leczenie nadwrażliwości zębiny), endodoncji, stomatologii dziecięcej, ortodoncji, periodontologii i leczeniu chorób błony śluzowej jamy ustnej, protetyce oraz chirurgii stomatologicznej. Ozonoterapia, zarówno w postaci mieszaniny wodnej, jak i w formie gazowej, wspomaga efekty terapeutyczne, jednak dopracowania wymagają standardy jej zastosowania

Evaluation of the Sensitivity of Selected Candida Strains to Ozonated Water—An In Vitro Study

(1) Background and Objectives: Oral candidiasis has increased significantly in recent years. Increasingly, we encounter treatment difficulties related to drug resistance. Therefore, it is necessary to search for other therapies such as ozone therapy, which has antimicrobial activity. The aim of this study was to determine the sensitivity of selected Candida strains to ozonated water based on concentration and contact time (2) Methods: The sensitivity of Candida strains to ozonated water with a concentration of 5 µg/mL, 30 µg/mL, and 50 µg/mL was assessed using Mosmann’s Tetrazolium Toxicity (MTT) assay. Statistical differences were assessed by the analysis of variance (ANOVA) and the Newman-Keuls post-hoc test. A p-value of ≤0.05 was considered to indicate a statistically significant difference. (3) Results: In all the strains and research trials, the number of viable cells was reduced by ozonated water. The reduction depended on the exposure time and concentration of ozonated water. The highest percentage reduction (34.98%) for the tested samples was obtained for the C. albicans strain after 120 s of exposure at the highest concentration-50 µg/mL. (4) Conclusions: The selected strains of Candida spp. were sensitive to ozonated water at all tested concentrations (5 µg/mL, 30 µg/mL, and 50 µg/mL). The sensitivity of strains to ozonated water increased with concentration and application time. Moreover, the sensitivity of Candida strains to ozonated water is comparable to that of 0.2% chlorhexidine gluconate

Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min