35 research outputs found
Rodent arena tracker (RAT): A machine vision rodent tracking camera and closed loop control system
Video tracking is an essential tool in rodent research. Here, we demonstrate a machine vision rodent tracking camera based on a low-cost, open-source, machine vision camera, the OpenMV Cam M7. We call our device the rodent arena tracker (RAT), and it is a pocket-sized machine vision-based position tracker. The RAT does not require a tethered computer to operate and costs about $120 per device to build. These features make the RAT scalable to large installations and accessible to research institutions and educational settings where budgets may be limited. The RAT processes incoming video in real-time at 15 Hz and save
Preparation of 1,2,3,4,7,7-Hexachl�robicyclo[2.2.1] hepta-2,5-diene by condensation of Hexachlorocyclopentadiene with acetylene
Determination of Bentazone, Inabenfide and Flusulfamide in Agricultural Products by HPLC and Confirmation by LC/MS.
Determination of Perchlorate Anion in Foods by Ion Chromatography−Tandem Mass Spectrometry
Lithiumaluminiumhydrid als Reagens zur mikroanalytischen Bestimmung funktioneller Gruppen
Lithiumaluminiumhydrid als Reagens zur mikroanalytischen Bestimmung funktioneller Gruppen
Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats
Single cell atlas of spinal cord injury in mice reveals a pro-regenerative signature in spinocerebellar neurons.
After spinal cord injury, tissue distal to the lesion contains undamaged cells that could support or augment recovery. Targeting these cells requires a clearer understanding of their injury responses and capacity for repair. Here, we use single nucleus RNA sequencing to profile how each cell type in the lumbar spinal cord changes after a thoracic injury in mice. We present an atlas of these dynamic responses across dozens of cell types in the acute, subacute, and chronically injured spinal cord. Using this resource, we find rare spinal neurons that express a signature of regeneration in response to injury, including a major population that represent spinocerebellar projection neurons. We characterize these cells anatomically and observed axonal sparing, outgrowth, and remodeling in the spinal cord and cerebellum. Together, this work provides a key resource for studying cellular responses to injury and uncovers the spontaneous plasticity of spinocerebellar neurons, uncovering a potential candidate for targeted therapy