Enzyme electrophoresis of South African Schistosoma mattheei and S. haematobium

As a pilot project of a study undertaken to determine the influence of S. mattheei X S. haematobium hybridization on various South African S. mattheei populations by means of biochemical-taxonomic methods, a comparative electrophoretic study of laboratory-maintained S. mattheei and S. haematobium was performed using 11 enzymes representing 16 gene loci. Eleven loci were found to be monomorphic, while 5 differed interspecifically. Computation of the results revealed that South African S. mattheei and S. haematobium are fairly closely related when compared with other Schistosoma spp. groups.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn2014mn201

Further observations on the electrophoretic characterization of South African Schistosoma mattheei and S. haematobium

Eleven enzymes, which were used to compare South African S. mattheei and S. haematobium in a former study, were employed to study intraspecific variation within S. mattheei, using starch gel electrophoresis and iso-electric focusing where resolution in starch gel was poor. Acid phosphatase varied intraspecifically within S. mattheei in that the most southern population differed from the northern populations. Malate dehydrogenase also varied intraspecifically. Three populations which occur sympatric with S. haematobium had a MDH-1 allele in common with the human schistosome while an allopatric population did not.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Host cellular components adhering to the tegument of schistosomes from cattle, buffalo, hippopotamus and lechwe

The teguments of adult Schistosoma mattheei from cattle and buffalo, S. hippopotami from hippopotamus and S. margrebowiei from lechwe were examined by means of scanning electron microscopy for cells possibly engaged in immunological action. Leukocytes were observed on the teguments of the schistosomes from all 4 host species. Although certain of these cells seemed to be fused to the surface membrane of the worms, they did not display pseudopodia. The tegument of certain schistosomes from buffalo exhibited cells the size of platelets with dendritic structures connected to the tegument of the parasite. The results seem to indicate that, as with laboratory hosts, naturally infected domestic and wild hosts are unable to mount an effective cellular response against the tegument of live adult schistosomes. The possible role of platelets in immunology against schistosomes is mentioned.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

The morphology of a sensory receptor in the nippled tubercles of Schistosoma mattheei

During scanning electron microscopy (SEM) of the tegument of Schistosoma mattheei, a structure was observed within the nippled tubercles. It is postulated that it is a sensory receptor with a tactile function.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

The occurrence of Schistosoma mattheei in the south-western Transvaal

To determine whether Schistosoma mattheei is present in the south-western Transvaal, sixty habitats were searched for the intermediate host snail, Bulinus africanus. Ten populations of this snail were located, 2 of which were infected with S. mattheei. Eggs of one of these isolates, originating from a spring in the Mooi River, were examined with an optical microscope. Scanning electron micrographs of the teguments of adult male worms and the terebratorial membranes of miracidia are described. These parasites are morphologically similar to some previously described from another habitat in the same geographical area and both populations can be regarded as typical S. mattheei.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

The tegument of Schistosoma hippopotami from Hippopotamus amphibius in the Kruger National Park

Schistosoma hippopotami were collected from the right heart chambers and pulmonary arteries of Hippopotamus amphibius culled in the Kruger National Park. The schistosomes were subjected to scanning electron microscopy as well as optical microscopy. The results indicate that S. hippopotami is not conspecific to S. mansoni as suggested in the literature. On account of the morphology of certain tegumental structures of both male and female parasites, it is suggested that S. hippopotami is adapted to the pulmonary arterial circulation of its host.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Notes on the occurrence of tubercular spines in Schistosoma margrebowiei and Schistosoma mattheei

Scanning electron microscopical (SEM) studies on tegument of the bovid schistosomes, Schistosoma margrebowiei and Schistosoma mattheei have yielded conflicting results; certain authors observed the tubercles on the tegument of these species to be spined, while others reported that they are spineless. The present study indicates that the protrusion of tubercular spines is subject to phenotypic plasticity regulated by external factors such as the identiy of the host species and whether or not the schistosome is paired.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Scanning electron microscopy of the teguments of males from five populations of Schistosoma mattheei

The teguments of males from 5 populations of S. mattheei, of which 3 were sympatric and 2 allopatric with S. haematobium, were studied by means of scanning electron microscopy (SEM). A certain percentage of the males of each sympatric population bore tubercle spines while the allopatric populations were spineless. It is postulated that the presence of tubercle spines is a characteristic inherited from S. haematobium.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

Phenotypic differences in Schistosoma mattheei ova from populations sympatric and allopatric to S. haematobium

Schistosoma mattheei ova were collected from cattle in different localities in South Africa and after hatching, miracidia were used to infest Bulinus (Physopsis) globosus. Cercariae harvested from these snails were used to infest the definitive host Praomys (Mastomys) coucha and eggs from the resulting female S. mattheei were collected. These ova were compared with a Schistosoma haematobium X S. mattheei hybrid similarly collected from an infested P. (M.) coucha. The results indicate that S. mattheei populations which are sympatric to S. haematobium possess S. haematobium characteristics. It is suggested that the gene pools of populations of the parasite in these areas are infiltrated with S. haematobium genes via the S. mattheei X S. haematobium hybrid originating from human hosts.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

Infrastructure for Detector Research and Development towards the International Linear Collider

The EUDET-project was launched to create an infrastructure for developing and testing new and advanced detector technologies to be used at a future linear collider. The aim was to make possible experimentation and analysis of data for institutes, which otherwise could not be realized due to lack of resources. The infrastructure comprised an analysis and software network, and instrumentation infrastructures for tracking detectors as well as for calorimetry.Comment: 54 pages, 48 picture