Hairy cell leukemia refractory to standard therapy with purine nucleoside analogs – case report and literature review

Hairy cell leukemia (HCL) is a rare, chronic lymphoproliferative disorder. Currently, purine nucleoside analogs (PNA) constitute the first line treatment of HCL. Cladribine could induce long lasting remission in majority of patients with only a single cycle of therapy. In fact the relapsed patients could be treated successfully with cladribine too. Sometimes we observe the resistance to PNA. Rituximab and chemoimmunotherapy (rituximab plus cladribine) are effective in treatment of refractory HCL.We present a case of a 64-year-old man who was treated with rituximab after second progression of HCL. In March 2011, rituximab was given at a dose of 375mg/m2 i.v. once a week for eight weeks, with result of achievement of PR. During the last hospitalization in March 2013 the persistence of PR was confirmed

Long-term response to azacitidine treatment in female with acute myeloid leukaemia and adverse cytogenetics – Case report

Acute myeloid leukemia (AML) is the most common type of leukaemia found in adults and the number of disease cases increases with age. Despite the advances in the AML treatment, the results in patients over the age of 60 remain unsatisfactory.In this study we present the case of a 73-year-old female patient with an unfavourable cytogenetic profile, in whom we observe long-term response to azacitidine, after previous failures of classic polychemotherapy.In February 2010, a 70-year-old patient was admitted to the Department of Haematology USK in Bialystok on suspicion of AML. The patient was qualified for intensive chemotherapy regimen of daunorubicin (DNR) and cytarabine (Ara-C).Cytogenetic examination revealed the presence of double minutes – acentric fragments of extrachromosomal DNA, which is associated with resistance to standard chemotherapy. Induction chemotherapy was complicated by febrile neutropenia, pneumonia and episodes of atrial fibrillation. Due to the lack of remission and severe after-induction period, a brief reinduction chemotherapy with DNR and Ara-C was applied to obtain complete remission with incomplete regeneration (CRi).Due to the recurrence in October 2010, reinduction chemotherapy was given followed by two cycles of maintenance chemotherapy. After another relapse in February 2011 (23,6% blasts in the bone marrow), a chemotherapy regimen designed for refractory and relapsed leukaemia was given, without any effect. In April 2011, the patient began azacitidine treatment. By the end of March 2013, the patient received twenty-one treatment cycles. The twelfth cycle of chemotherapy was complicated by pulmonary embolism which was treated successfully. The complete blood count remains at normal values.Recent reports indicate a clear relationship processes such as epigenetic regulation of DNA methylation with leukaemogenesis. The use of hypomethylating drugs in AML is yielding promising results

Recovery of volatile organic compounds from waste gases in TSA system with fixed bed of polymeric adsorbent

Przedstawiono wyniki modelowania matematycznego oraz badań doświadczalnych procesu odzyskiwania lotnych związków organicznych (VOCs) z powietrza w układzie adsorpcyjnym zmiennotemperaturowym (TSA) z nieruchomym złożem adsorbentu polimerowego. Cykl TSA składał się z trzech etapów: adsorpcji związku organicznego z powietrza, desorpcji za pomocą strumienia ogrzanego azotu oraz chłodzenia złoża z użyciem strumienia chłodnego azotu. Analizę zmian czasowych stężenia i temperatury podczas adsorpcji, desorpcji i chłodzenia wykonano za pomocą modelu matematycznego adsorpcji nieizotermicznej, nierównowagowej. Badania doświadczalne wykonano przy użyciu laboratoryjnej instalacji adsorpcyjnej. Badania wykonano dla dwóch związków organicznych: toluenu i izopropa-nolu. Jako adsorbent stosowano polimer Dowex Optipore V503. Porównano wyniki uzyskane w układach TSA z zamkniętym i otwartym obiegiem gazu.The theoretical and experimental studies on the volatile organic compound (VOCs) recovery from air in the thermal swing adsorption (TSA) systen with fixed bed of polymeric adsorbent are presented. The TSA cycle wa operated in three steps: an adsorption of organic compound from air, a de sorption step with hot nitrogen, and a cooling of adsorbent bed with coli nitrogen. A nonisothermal, nonequilibrium mathematical model was used ti simulate temperature and concentration data for adsorption, desorption am cooling steps. A bench scale fixed bed adsorption unit was used for the expe rimental study. Toluene and isopropanol were chosen as a volatile organi compounds. Dowex Optipore V503 was selected as adsorbent. The result obtained in closed-loop and open-flow TSA systems were compared

Intestinal parasites of monkeys from the Zoological Garden in Wroclaw

Ceprologic examination of representatives of 9 monkey species carried out in the Wrocław Zoo revealed the folloving intestinal parasittes: Trichocephalus trichiurus, Strongyloides stercoralis, Nematodirus weinbergi, Ascaris lumbricoides, Enterobius vermicularis and Capillariidae gen. sp. The total extensity of infection was 80%, however intensity was rather low, except for E. vermicularis found in chimpanzees. It seems, that nematoda present the most important problem in control of parasitic infection in zoological gardens

Modeling of isopropanol recovery from air stream in the TSA system with a fixed bed of polymeric adsorbent

Przedstawiono wyniki analizy teoretycznej procesu odzyskiwania izopropanolu w układzie adsorpcyjnym zmienno-temperaturowym (TSA) z nieruchomym złożem adsorbentu polimerowego. Cykl TSA składał się z trzech etapów: adsorpcji związku organicznego z powietrza, desorpcji za pomocą strumienia ogrzanego azotu oraz chłodzenia złoża z użyciem strumienia chłodnego azotu. Analizę zmian czasowych stężenia i temperatury podczas adsorpcji, desorpcji i chłodzenia wykonano za pomocą modelu matematycznego adsorpcji nieizotermicznej, nierównowagowej. Do opisu kinetyki adsorpcji zastosowano model liniowej siły napędowej (LDF). Celem przeprowadzonej analizy było zbadanie wpływu temperatury gazu na wlocie do złoża oraz na wylocie ze skraplacza podczas desorpcji na aktywność użyteczną złoża. Jako adsorbent wybrano polimer Dowex Optipore V503.Results of theoretical analysis of isopropanol recovery in the thermal swing adsorption (TSA) system with a fixed bed of polymeric adsorbent are presented. The TSA cycle consisted of three steps: adsorption of organic compound from air, desorption with hot nitrogen, and cooling of the adsorbent bed with cold nitrogen. A nonisothermal, nonequilibrium mathematical model was used to simulate temperature and concentration data for adsorption, desorption and cooling steps. The LDF-type model was used in the description of adsorption kinetics. The aim of analysis was to study an influence of gas temperatures in the bed inlet and condenser outlet during desorption on the effective activity of adsorbent bed. Dowex Optipore V503 was selected as adsorbent

Osmotic and heat stresses in in vitro anther culture of barley

Zmianę drogi rozwoju mikrospor z gametofitycznej na sporofityczną stymuluje się działając czynnikami stresowymi, którym poddaje się kłosy lub pylniki przed kulturą in vitro. U jęczmienia najczęściej stosuje się stres chłodzenia (4°C) lub stres osmotyczny przy pomocy 0,3 mol·dm⁻³ mannitolu. Celem pracy było zbadanie wpływu na androgenezę różnych stężeń mannitolu w połączeniu z temperaturą 25°C i 33°C. U odmiany modelowej Igri udowodniono, że przy traktowaniu 0,3 mol·dm⁻³ mannitolem temperatura 33°C działała negatywnie na liczbę uzyskanych zarodków i roślin, a szczególnie na przekształcanie się zarodków w zielone rośliny. Przy wyższym stężeniu mannitolu (0,7 mol·dm⁻³) temperatura miała mniejsze znaczenie. U innych odmian, u których badano wpływ temperatury tylko przy niższym stężeniu mannitolu, reakcja na podwyższenie temperatury była podobna. U wszystkich badanych odmian stężenie 0,7 mol·dm⁻³ mannitolu było korzystniejsze niż 0,3 mol·dm⁻³: mimo ograniczenia liczby struktur embriogennych wpłynęło dodatnio na ich dalszy rozwój. Zastosowanie wyższego stężenia mannitolu (1,0 mol·dm⁻³, 1,5 mol·dm⁻³) w temperaturze 25°C nie zwiększyło liczby roślin zielonych.The change in the developmental pathway of microspores from gameto- phytic to sporophytic onr is stimulated by stress applied to spikes or anthers before in vitro culture. In barley, two most commonly used types of stress are chilling (4°C) and osmotic stress by 0.3 mol·dm⁻³ mannitol. The research aimed at determining the effect of various mannitol concentrations combined with the temperature of 25 or 33°C. In the model Igri cultivar, the temperature 33°C combined with 0.3 mol·dm⁻³ mannitol treatment were found to have an harmful effect on the number of obtained embiyos and plants, and particularly on the development of embryos into green plants. At higher mannitol concentration (0.7 mol·dm⁻³), the temperature was less important. In other cultivars, in which the effect of temperature was examined only at lower mannitol concentration, the response to increased temperature was similar. In all the tested cultivars, 0.7 mol·dm⁻³ mannitol concentration was more favourable than 0.3 mol·dm⁻³: despite limiting the number of embryo-like structures, it showed a positive effect on their further development. Using of higher mannitol concentrations (1.0 mol·dm⁻³, 1.5 mol·dm⁻³) at 25°C did not increase the number of green plants in comparison to 0.7 mol·dm⁻³

Intestinal parasites of monkeys from the Zoological Garden in Wroclaw

Ceprologic examination of representatives of 9 monkey species carried out in the Wrocław Zoo revealed the folloving intestinal parasittes: Trichocephalus trichiurus, Strongyloides stercoralis, Nematodirus weinbergi, Ascaris lumbricoides, Enterobius vermicularis and Capillariidae gen. sp. The total extensity of infection was 80%, however intensity was rather low, except for E. vermicularis found in chimpanzees. It seems, that nematoda present the most important problem in control of parasitic infection in zoological gardens

Androgenic potential of polish barley cultivars in anther culture in vitro

The aim of the research was to assess the androgenic potential of 21 Polish cultivars of spring and winter barley. On average, a few green plants developed per 100 anthers. Cold pretreatment proved conducive to the formation of embryos/calli, while mannitol pretreatment favoured green plant development. The first embryos/calli formed on the induction medium developed most efficiently. The authors chose FHG medium supplemented with 1 mg/l BAP and 0.2 mg/l 2,4-D (induction of androgenesis) and FHG medium supplemented with 0.4 mg/l BAP and 3% maltose (plant development)