MINIMALIZACIJA TRANSPORTNIH TROŠKOVA

U ovom članku autor daje kratak prikaz rezultata linearnog programiranja i njegovu primjenu. Zatim na jednom primjeru prijevoza opeke, na osnovi primjene jedne transportne metode, prikazuje utvrđivanja minimalnih transportnih troškova. Primjer može poslužiti kadrovima koji rade kao disponenti motornih vozila za prijevoz tereta u transportnim organizacijama udruženog rada

Svjesnost, stavovi i kupovina hrane s oznakama kvalitete u Sloveniji

Purpose – This study aims to identify the awareness of Slovenian consumers and their attitudes towards labels, as well as the reasons why consumers do not buy labeled food. Design/Methodology/Approach – A qualitative approach involving six focus groups (N = 60) and shopping with consumers (N = 16) was conducted in the winter of 2020/2021. Thematic analysis was used to analyze the data. Findings and implications – The results show that the awareness of quality labels is weak mainly due to their considerable number and poor designed, but also on account of ineffective communication campaigns. With respect to the awareness of and attitude to the quality scheme, the participants were divided into four groups: “The Aware”, “The Sceptics”, “Potential Buyers”, and “The Disinterested”. The reasons for not buying quality labeled foods despite being aware of the labels included overpriced products, no availability of labeled foods generally and those with a good taste and special nutritional value specifically, familiarity/experience with the product, and mistrust of the labeling and social system. The study stresses the important role of social perception and trust in the social system for the acceptance and purchase of such food. Limitations – The main limitation of the study is its focus on digitally literate consumers only. Originality – The relevant literature is enhanced by considering the shopping with consumers exercise given that no published study to date, according to the authors’ knowledge, is based on the observation of quality labels at the time of purchase.Svrha – Cilj studije je identificirati svjesnost slovenskih potrošača, njihove stavove prema oznakama kvalitete i razloge zašto ne kupuju označenu hranu. Metodološki pristup – Provedeno je kvalitativno istraživanje sa šest fokus grupa (N = 60) i tijekom kupovine s potrošačima (N = 16) u zimi 2020./2021. Za analizu podataka korištena je tematska analiza. Rezultati i implikacije – Rezultati pokazuju da je svjesnost o oznakama kvalitete slaba uglavnom zbog činjenice da ih ima izuzetno puno, loše su osmišljene i zbog neučinkovitosti komunikacijskih kampanji. S obzirom na svjesnost i stav prema kvaliteti, sudionici istraživanja su podijeljeni u četiri skupine: “Svjesni”, “Skeptici”, “Potencijalni kupci” i “Nezainteresirani”. Razlozi zbog kojih se ne kupuje hrana s oznakom kvalitete, usprkos svjesnosti o oznakama, jesu precijenjeni proizvodi i nedostatak dostupnosti hrane s oznakama općenito, a posebno zbog dobrog okusa i posebnih nutritivnih vrijednosti, poznavanja/iskustva s proizvodom i nepovjerenja u označavanje i društveni sustav. Za kupovinu i prihvaćanje takve hrane studija naglašava važnu ulogu društvene percepcije i povjerenja u društveni sustav. Ograničenja – Glavno ograničenje istraživanja jest u tome što je usmjereno na digitalno pismene potrošače. Doprinos – Relevantna literatura obogaćena je razmatranjem kupovine s potrošačima s obzirom na to da se ni jedna do danas objavljena studija, prema saznanjima autora, ne temelji na promatranju oznaka kvalitete u trenutku kupovine

Revitalization of the Slovenian Herbal Market? A Mixed Study Approach

Purpose – The study aims to identify the needs, barriers, and measures relevant for the revitalization of the herbal market in Slovenia. Design/Methodology/Approach – A mixed-methods approach was used in the study, with a quantitative online survey of Slovenian herbalists (N = 217) and in-depth interviews conducted with key Slovenian stakeholders in the field of herbalism (N = 18). Findings and Implications – Most Slovenian herbalists grow herbs as a hobby or as a supplementary farm activity; they have little land for growing herbs and thus also generate a low income. Most of them sell their products directly to consumers, with only one tenth selling herbs to retailers. As many as three quarters of the respondents are not a part of any organization which, along with lower prices, is the main barrier to a market revitalization. The in-depth interviews revealed a lack of interest in collaboration among herbalists because they find the existing situation to be sufficient to a certain extent, as demand is greater than their supply. The results show no evidence of an herbal market as it existed before Slovenia’s independence. Limitations – Since there is no census of Slovenian herbalists, the sample is not representative. Originality – There is a lack of understanding of the barriers to and measures for revitalizing the herbal market, especially in developed countries whose market was well developed decades ago, such as Slovenia

Složena biokemija i biotehnološka proizvodnja betalaina

The demand for natural food colourants is increasing because of public awareness of their health benefits. Betalains are nitrogen-containing plant pigments whose colours range from red-violet betacyanins to yellow betaxanthins. They are used for colouring dairy products, meat and frozen desserts. Betalains have attracted additional interest because of their antioxidative, anti-inflammatory and anticarcinogenic properties. The main source of commercially produced betalains is red beet root, but alternative sources are found in plants from the Amaranthaceae and Cactaceae families. Another alternative source is plant cell culture in bioreactors, although optimization of pigment production seems necessary. In this paper we synthesize the results of recent studies on betalain biosynthesis, chemical properties, sources, biotechnology and applications.Sve je veća potražnja za prirodnim bojilima zbog njihovih zdravstvenih pogodnosti. Betalaini su biljni pigmenti koji sadrže dušik, s rasponom boja od crveno-ljubičastih betacijana do žutih betaksantina. Koriste se za bojanje mliječnih proizvoda, mesa i smrznutih deserta. Zanimljivi su i zbog svoga antioksidativog, protuupalnog i antikancerogenog djelovanja. Glavni komercijalni izvor betalaina je korijen cikle, a alternativni izvori pigmenta nalaze se u biljkama iz porodica Amaranthaceae i Cactaceae. Biljne stanice uzgajane u bioreaktorima također su izvor pigmenata iako je potrebno daljnje optimiranje njihove proizvodnje. U ovom su radu sažeti rezultati nedavnih istraživanja biosinteze betalaina, njihovih kemijskih svojstava, izvora, biotehnologije i uporabe

Crown-gall tumor kao test za određivanje antitumorske aktivnosti proteina imele (Viscum album L.)

The crown gall tumor test system on potato tuber discs was used for the study of antiumor activity of mistletoe protein extracts. By estimating the number and weight of tumors we found that crude mistletoe protein extract inhibits tumor induction and growth. The refined protein fraction was even more efficient. The mistletoe proteins also showed stimulative effects on rhizogenesis.Crown-gall tumori na tkivu gomolja krumpira korišteni su kao test-sistem za ispitivanje antitumorske aktivnosti proteina u ekstraktu imele. Određivanje broja i mase tumora pokazalo je, sa statističkom sigurnošću, da već sirovi ekstrakt inhibira tumorsku indukciju i rast. Inhibitomo djelovanje pročišćenih proteinskih frakcija još je izrazitije. Proteinski ekstrakt stimulirao je rizogenezu u tumorskom tkivu

Protein glycosylation in sugar beet cell line can be influenced by DNA hyper- and hypomethylating agents

Protein glycosylation is a co- and post-translational modification that influences protein function, stability and localization. Changes in glycoprotein pattern during differentiation/dedifferentiation events exist in animal cells and DNA methylation status is closely related to the changes. However, in plant cells this relationship is not yet established. In order to verify whether such a relation exists, hypermethylating drugs 2,4-dichlorophenoxyacetic acid and hydroxyurea, or hypomethylating drug 5-azacytozine were applied to sugar beet (Beta vulgaris L.) cells during 14 days of in vitro subculture, and the glycoprotein patterns of the cells were compared. The applied drugs were not toxic, as observed from cell phenotype and by measuring growth of the control and treated cells. Hyper and hypomethylating treatments influenced the activity of enzymes related to differentiation state of the cells: peroxidases and esterases, and their isoform patterns. Electrophoretic patterns of soluble and membrane proteins were similar between control and treatments, but the treatments modified N- and O-linked glycoprotein patterns as visible from GNAand PNAlectin blots. This suggested that hypermethylation and hypomethylation of genomic DNA in sugar beet cells affect protein glycosylation patterns and cellular metabolism, possibly in a mechanism similar to that existing in animal cells

Electrophoretic protein patterns and peroxidase activity related to morphogenesis in Mammillaria gracillis tissue culture

The cactus Mammillaria gracillis Pfeiff. was propagated in vitro on Murashige and Skoog (MS) medium without any growth regulators. At the bases of some plants abundant callus masses developed. Lower agar and higher MS salt concentrations stimulated callus production. In the culture, morphologically normal and hyperhydric shoots were regenerated. The growth of crown-gall tumour was induced on disc-like explants of in vitro grown plants infected with Agrobacterium tumefaciens, B6S3 strain. Calli appeared on both infected and control explants. The tumorous tissue grew more intensively than the control. The transformed character of the callus was confirmed by PCR amplification of a gene 6a of T-DNA. Gene expression in cactus shoots (grown in vitro or in pot), callus, hiperhydric and morphologically normal regenerated shoots and crown-gall tumour were analysed at the level of the electrophoretic pattern of soluble proteins. Some tumour-specific polypeptides were detected (76, 32-39, and 23 kDa). That of 42 kDa was highly expressed in callus and hyperhydric shoots. A faint 58 kDa band was found in all extracts except in the pot-grown shoot. Relatively high peroxidase activity was detected in callus and shoot regenerants and it was lower in tumour and the lowest in plant shoots

Tumorska transformacija u tkivu gomolja krumpira

Potato (Solarium tuberosum L. cv. Desirée) tuber tissue was infected with Agrobacterium tumefaciens, strains: B6S3 (wild type), pGV2255 (rooty mutant), pGV2215 (shooty mutant) and with Agrobacterium rhizogenes (8196). Successful tumor induction was achieved by B6S3 (unorganised tumors) and by pGV2255 (rooty tumors). Transformed tuber cells were smaller than normal ones and free from starch. Transformation process caused a significant increase in peroxidase activity which can be taken as a tumorigenetic marker. Electrophoretic protein pattern showed that tumor cells stopped producing a polypeptide of about 40 kd, which was a storage protein dominant in normal tissue.Tkivo gomolja krumpira inficirale smo bakterijom Agrobacterium tumefaciens (sojevi: B6S3, pGV2215, pGV2255) i A. rhizogenes (8196). Uspješnu tumorsku transformaciju postigle smo divljim sojem (B6S3) i mutiranim (pGV2255) koji izaziva tumore s korijenom. Svjetlosnim mikroskopom mogle smo razlikovati normalne stanice od tumorskih. Tumorska transformacija odrazila se u porastu peroksidazne aktivnosti te stoga, ovaj enzim može poslužiti kao pokazatelj transformacijskih procesa. Elektroforetskim razdvajanjem topivih proteina u SDS-poliakrilamidnom gelu utvrdile smo da tumorske stanice prestaju sintetizirati proteinsku prugu na položaju koji odgovara molekularnoj težini od oko 40 kd