Book Reviews

Book reviews of: William F. Winter and the New Mississippi: A Biography. By Charles C. Bolton Jackson: University Press of Mississippi, 2013. Pp. vii, 368. Illustrations, map, acknowledgements, notes, index. $35.00 cloth. ISBN: 9781617037870. Born of Conviction: White Methodists and Mississippi’s Closed Society. By Joseph T. Reiff. (New York: Oxford University Press, 2016. Acknowledgements, illustrations, map, notes, index. Pp. xxi, 384.$35 Hardcover. ISBN: 9780190246815). In Katrina’s Wake: The U.S. Coast Guard and the Gulf Coast Hurricanes of 2005. By Donald L. Canney. (Gainesville: University Press of Florida, 2010. Foreword, notes, index. Pp. xv, 228. $27.50 cloth.) Slavery, Race and Conquest in the Tropic: Lincoln, Douglas and the Future of America. By Robert E. May. (New York: Cambridge University Press, 2013. Acknowledgements, illustrations, maps, notes, index. Pp. xi, 296.$80 cloth, $26.99 paper,$22 e-book. ISBN: 9780521132527.) Rivers of Sand: Creek Indian Emigration, Relocation, and Ethnic Cleansing in the American South. By Christopher D. Haveman. (Nebraska: University of Nebraska Press, 2016. Illustrations, preface, acknowledgments, notes on terminology, index. Pp. ix, 414. Trouble in Goshen: Plain Folk, Roosevelt, Jesus, and Marx in the Great Depression. By Fred C. Smith (Jackson: University Press of Mississippi, 2014. Acknowledgements, illustrations, map notes, index. Pp. xi, 214. $60.00 cloth. ISBN: 9781617039560.) Builders of a New South: Merchants, Capital, and the Remaking of Natchez, 1865-1914. By Aaron D. Anderson (Jackson: University Press of Mississippi, 2013. Acknowledgments, illustrations, photographs, notes, graphics, index. Pp. 279.$40 cloth. ISBN: 978-1- 61703-667-5.) Adventurism and Empire: The Struggle for Mastery in the Louisiana- Florida Borderlands 1762-1803. By David Narrett. (Chapel Hill: The University of North Carolina Press, 2015. Acknowledgements, illustrations, notes, index. Pp. xi, 365. $45 cloth,$44.99 e-book. ISBN: 978-1-4696-1833-3.) Empty Sleeves: Amputation in the Civil War South. By Brian Craig Miller. (Athens: University of Georgia Press, 2015. Illustrations, acknowledgments, appendix, notes, index. Pp. xvi, 257. $79.95 cloth,$29.95 paper. ISBN: 0820343327.) Signposts: New Directions in Southern Legal History. By Sally E. Hadden and Patricia Hagler Minter, eds. (Athens and London: The University of Georgia Press, 2013. Acknowledgements, illustrations, index. Pp. xi, 480. $69.95 cloth,$26.95 paper, $26.95 ebook. ISBN: 978-0-8203-4499-7.) The Color of Christ: The Son of God and the Saga of Race in America. By Edward J. Blum and Paul Harvey. (Chapel Hill: University of North Carolina Press, 2012. Pp. 325.$32.50 Cloth. ISBN: 9780807835722.

Use of Fluorescence-activated Vesicle Sorting for Isolation of Naked2-associated, Basolaterally Targeted Exocytic Vesicles for Proteomics Analysis*S⃞

By interacting with the cytoplasmic tail of a Golgi-processed form of transforming growth factor-α (TGFα), Naked2 coats TGFα-containing exocytic vesicles and directs them to the basolateral corner of polarized epithelial cells where the vesicles dock and fuse in a Naked2 myristoylation-dependent manner. These TGFα-containing Naked2-associated vesicles are not directed to the subapical Sec6/8 exocyst complex as has been reported for other basolateral cargo, and thus they appear to represent a distinct set of basolaterally targeted vesicles. To identify constituents of these vesicles, we exploited our finding that myristoylation-deficient Naked2 G2A vesicles are unable to fuse at the plasma membrane. Isolation of a population of myristoylation-deficient, green fluorescent protein-tagged G2A Naked2-associated vesicles was achieved by biochemical enrichment followed by flow cytometric fluorescence-activated vesicle sorting. The protein content of these plasma membrane de-enriched, flow-sorted fluorescent G2A Naked2 vesicles was determined by LC/LC-MS/MS analysis. Three independent isolations were performed, and 389 proteins were found in all three sets of G2A Naked2 vesicles. Rab10 and myosin IIA were identified as core machinery, and Na+/K+-ATPase α1 was identified as an additional cargo within these vesicles. As an initial validation step, we confirmed their presence and that of three additional proteins tested (annexin A1, annexin A2, and IQGAP1) in wild-type Naked2 vesicles. To our knowledge, this is the first large scale protein characterization of a population of basolaterally targeted exocytic vesicles and supports the use of fluorescence-activated vesicle sorting as a useful tool for isolation of cellular organelles for comprehensive proteomics analysis