IN VITRO PROPAGATION OF SHOOTS AND CALLUS INDUCTION OF GYMNEMA SYLVESTRE R. BR. AN IMPORTANT ANTI-DIABETIC PLANTâ€

Objective: The objective of this research was to establish and develop a protocol for the mass multiplication and callus induction of an anti-Diabetic plant-G. sylvestre R. Br.Methods: Sterilized explants (Nodal segment and leaf) were used for the initiation of culture. They were cultured on MS medium supplemented with a variety of PGRs (BAP, Kn, IBA, 2,4-D) individually or in combinations.Results: The induction of multiple shoots from nodal segments were highest in MS medium supplemented with 2.0 mg/l Kn and in BAP Maximum shoots were obtained on MS medium fortified with 1 mg/l BAP. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium supplemented with 2.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to mist house. Leaf petiole explants were used for the purpose of callus induction. Best growth was observed in MS medium supplemented with 2,4-D. 1.0 mg/l 2,4-D+0.5 mg/l BAP, 1.0 mg/l 2,4-D+1.5 mg/l Kn.Conclusion: The results obtained in this research work clearly indicated that Kn is a better choice than BAP for the culture initiation. 2 mg/l IBA was proved best for root induction. For callus induction, 1 mg/l 2,4-D gave good results and when callus was sub-cultured on 2,4-D with BAP or Kn then 1.0 mg/l 2, 4-D+1.5 mg/l Kn proved best for mass propagation of callus

Pharmacognostic evaluation of stem bark and leaves of Anogeissus pendula Edgew

Background: Anogeissus pendula Edgew has ethnomedicinal importance in various parts of India. It is used in diarrhoea, dysentery, cough, wound healing, burns, skin diseases and gastric disorder. No attempts have been made regarding pharmacognostic investigation of the plant till date. Aim: The present study aimed to perform the pharmacognostic study of leaf and stem bark of Anogeissus pendula Edgew. Settings and Design: The study was designed in accordance with standard procedures. Materials and Methods: Pharmacognostic studies viz. organoleptic, macroscopic, microscopic, physicochemical and chromatographic fingerprinting on fresh and dried plant parts along with the hydroalcoholic extracts were conducted. Results and Conclusions: A. pendula has dorsiventral oblanceolate to obovate leaves arranged in opposite pairs while the stem bark has rough appearance and ash to greyish white in colour. Leaves have anomocytic type of stomata. The characteristic microscopic features of leaves were observed to be epidermal cells, palisade and spongy parenchyma, vascular bundles, xylem and phloem cells. Stem bark microscopy revealed the presence of periderm, secondary cortex and secondary phloem. Chromatographic fingerprinting of extracts showed the presence of flavonoids (Rutin and Quercetin) and they were quantified. Rutin was found to be 6.23% w/w in leaves and 9.97% w/w in stem bark extract while Quercetin to be 27.29% w/w and 51.62% respectively. The present study evaluated various pharmacognostic parameters which will help in quality control (standardization) of A. pendula Edgew in crude form, in herbal formulations and also aid in the preparation of an herbal monograph for the species