Evaluation of a Cold Staining Method for Acid-Fast Bacilli in Sputum

Comparison between the Ziehl-Neelscn staining method for acid-fast bacilli, applied with and without heating, was carried out in a controlled investigation using smears prepared from 306 sputum samples collected prior to treatment from suspected cases of pulmonary tuberculosis. Smear and culture positively were graded and the colour intensity of bacilli recorded. Results showed that the chance corrected agreement (Kappa) between Z-N and cold methods was only 78%. The sensitivity of the Z-N and cold methods were 84% and 77% respectively when compared with culture results. Assuming 10% smear positivity among symptomatics reporting to Peripheral Health Institutions (PHIs), the positive predictive value of the cold method was very low(53%). When compared to culture, the positive predictive value is 71% for the Z-N method and 57% for the cold method for a symptomatic population with 15% culture positivity. In the absence of heating. penetration of the stain was significantly reduced and consequently the number of bacilli detected was less. The inability to take the stain without heating was seen in smears from all grades of culture positive samples: thus even heavy positives were missed by the cold method. The evaluation of the cold method against the standard Z-N method highlights its limitations and demonstrates that it is not as reliable as the standard Z-N method

Coarsening and Slow-Dynamics in Granular Compaction

We address the problem of the microscopic reorganization of a granular medium under a compaction process in the framework of Tetris-like models. We point out the existence of regions of spatial organization which we call domains, and study their time evolution. It turns out that after an initial transient, most of the activity of the system is concentrated on the boundaries between domains. One can then describe the compaction phenomenon as a coarsening process for the domains, and a progressive reduction of domain boundaries. We discuss the link between the coarsening process and the slow dynamics in the framework of a model of active walkers on active substrates.Comment: Revtex 4 pages, 4 figures, in press in PRL. More info http://axtnt3.phys.uniroma1.it/Tetri

Restriction fragment length polymorphism of Mycobacterium tuberculosis strains from various regions of India, using direct repeat probe

Intraspecies differentiation was studied on 68 M. tuberculosis strains obtained from 6 states of India by restriction fragment length polymorphism (RFLP) using a direct repeat probe (DR probe) hybridised with Alu I digest of DNA. Most strains showed polymorphism based patterns that comprised between 2 to 7 bands and were grouped into 26 RFLP types. Of the 11 strains tested from Amritsar, 8 were RFLP type 5; the remaining 3 were of type 11 and were exclusively confined to this region. The strains from other regions were more heterogeneous. We confirm that DR-associated RFLP can be an excellent tool for the differentiation of M. tuberculosis strains. Depending on their geographical origin, these strains can be differentiated to a large extent by DR fingerprinting

Synthesis of thujane

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Early bactericidal action of pulsed exposure to rifampicin, ethambutol, isoniazid & pyrazinamide in pulmonary tuberculosis patients

The bactericidal action of two therapeutic regimens on Mycobacterium tuberculosis was assessed by viable counts in serial sputum samples in 49 pulmonary tuberculosis patients being treated with rifampicin (R), etbambutol (Emb), isoniazid (I) and pyrazinamide (Z) together in a single dose thrice weekly (REmbIZ3) or with REmb and IZ on alternate days (REmb3IZ3alt). In both groups of patients, there was a significant reduction (P < 0.02) in the colony forming units (cfu) of M. tuberculosis per ml of sputum during the first two days of treatment itself. This early bactericidal action (EBA) as well as the reduction in counts during the subsequent days of treatment were similar (P > 0.2) for both REmbIZ3 and REmb3IZ3alt regimens indicating that splitting up REmbIZ into REmb on one day and IZ on the next day in short course chemotherapy (SCC) regimens may not affect the bactericidal action of the regimens

"Exhaustion" Physics in the Periodic Anderson Model using Iterated Perturbation Theory

We discuss the "exhaustion" problem in the context of the Periodic Anderson Model using Iterated Perturbation Theory(IPT) within the Dynamical Mean Field Theory. We find that, despite its limitations, IPT captures the exhaustion physics, which manifests itself as a dramatic, strongly energy dependent suppression of the effective Anderson impurity problem. As a consequence, low energy scales in the lattice case are strongly suppressed compared to the "Kondo scale" in the single-impurity picture. The IPT results are in qualitative agreement with recent Quantum Monte Carlo results for the same problem.Comment: 13 preprint pages including 1 table and 4 eps figures, replaced by revised version, accepted for publication in Europhysics Letters, added references and conten

Eulerian Walkers as a model of Self-Organised Criticality

We propose a new model of self-organized criticality. A particle is dropped at random on a lattice and moves along directions specified by arrows at each site. As it moves, it changes the direction of the arrows according to fixed rules. On closed graphs these walks generate Euler circuits. On open graphs, the particle eventually leaves the system, and a new particle is then added. The operators corresponding to particle addition generate an abelian group, same as the group for the Abelian Sandpile model on the graph. We determine the critical steady state and some critical exponents exactly, using this equivalence.Comment: 4 pages, RevTex, 4 figure

Group Differential Privacy-Preserving Disclosure of Multi-level Association Graphs

Traditional privacy-preserving data disclosure solutions have focused on protecting the privacy of individual's information with the assumption that all aggregate (statistical) information about individuals is safe for disclosure. Such schemes fail to support group privacy where aggregate information about a group of individuals may also be sensitive and users of the published data may have different levels of access privileges entitled to them. We propose the notion of Eg-Group Differential Privacy that protects sensitive information of groups of individuals at various defined privacy levels, enabling data users to obtain the level of access entitled to them. We present a preliminary evaluation of the proposed notion of group privacy through experiments on real association graph data that demonstrate the guarantees on group privacy on the disclosed data

The Strong Coupling Fixed-Point Revisited

In recent work we have shown that the Fermi liquid aspects of the strong coupling fixed point of the s-d and Anderson models can brought out more clearly by interpreting the fixed point as a renormalized Anderson model, characterized by a renormalized level $\tilde\epsilon_d$, resonance width, $\tilde\Delta$, and interaction $\tilde U$, and a simple prescription for their calculation was given using the numerical renormalization group (NRG). These three parameters completely specify a renormalized perturbation theory (RPT) which leads to exact expressions for the low temperature behaviour. Using a combination of the two techniques, NRG to determine $\tilde\epsilon_d$, $\tilde\Delta$, and $\tilde U$, and then substituting these in the RPT expressions gives a very efficient and accurate way of calculating the low temperature behaviour of the impurity as it avoids the necessity of subtracting out the conduction electron component. Here we extend this approach to an Anderson model in a magnetic field, so that $\tilde\epsilon_d$, $\tilde\Delta$, and $\tilde U$ become dependent on the magnetic field. The de-renormalization of the renormalized quasiparticles can then be followed as the magnetic field strength is increased. Using these running coupling constants in a RPT calculation we derive an expression for the low temperature conductivity for arbitrary magnetic field strength.Comment: Contribution to JPSJ volume commemorating the 40th anniversary of the publication of Kondo's original pape