Prospects for Improving Alfalfa Yield Using Genomic- and Phenomic-Based Breeding

Alfalfa (Medicago sativa L.) is a perennial outcrossing legume that is cultivated as an important forage crop in many parts of the world. Yield is the most important trait for profitable alfalfa production, yet over the last 30 years yield improvement in California has stagnated. Current breeding methods focus on recurrent phenotypic selection; however, alternatives incorporating genomic- and phenomic-based information may enhance genetic gain and help to address the lack of yield improvement. Here we attempt to increase the yield potential of alfalfa using genomic selection (GS) in combination with high throughput phenotyping (HTP). A total of 193 families from two closely related elite populations were sown in the greenhouse and transplanted into mini sward plots at two locations near Davis, CA in May 2020. The trial was managed as a high-input system under full irrigation. Families were genotyped and phenotyped for biomass yield by mechanical harvest and a combination of drone and tower-based remote sensors across 12 harvests, 3 in the establishment year (2020), 7 in the first full year of production (2021) and 2 in 2022. Alfalfa yields ranged from 13-27 tonnes DM/hectare/year with a number of half-sib families outperforming popular cultivars in the first 2 years of production. Biomass volume predicted from the drone-based cameras had a moderate prediction accuracy with an overall R2 of 0.55. Some individual harvests reached accuracies as high as 0.85. Genotyping resulted in a dataset with 6,838 SNPs. Allele frequencies were used to generate a relationship matrix for GS. Narrow-sense heritability for dry matter yield was 0.31 and the predictive ability of the GS model was 0.15

Glial Hsp70 Protects K+ Homeostasis in the Drosophila Brain during Repetitive Anoxic Depolarization

Neural tissue is particularly vulnerable to metabolic stress and loss of ion homeostasis. Repetitive stress generally leads to more permanent dysfunction but the mechanisms underlying this progression are poorly understood. We investigated the effects of energetic compromise in Drosophila by targeting the Na+/K+-ATPase. Acute ouabain treatment of intact flies resulted in subsequent repetitive comas that led to death and were associated with transient loss of K+ homeostasis in the brain. Heat shock pre-conditioned flies were resistant to ouabain treatment. To control the timing of repeated loss of ion homeostasis we subjected flies to repetitive anoxia while recording extracellular [K+] in the brain. We show that targeted expression of the chaperone protein Hsp70 in glial cells delays a permanent loss of ion homeostasis associated with repetitive anoxic stress and suggest that this is a useful model for investigating molecular mechanisms of neuroprotection

Phylogenetic and molecular characteristics of Eurasian H9 avian influenza viruses and their detection by two different H9-specific RealTime reverse transcriptase polymerase chain reaction tests

Avian influenza viruses (AIVs) of the H9 haemagglutinin subtype are endemic in many Asian and Middle-East countries, causing mortality and morbidity in poultry. Consequently there is a need for accurate and sensitive detection of Eurasian H9 subtype viruses. Two H9 RealTime reverse transcriptase polymerase chain reaction (RRT-PCR) tests, developed by Monne et al. (2008) and Ben Shabat et al. (2010), were originally validated with a limited number of H9 specimens. In the present study, the two tests have been assessed using 66 diverse H9 isolates and 139 clinical specimens from six H9 poultry outbreaks in four geographically disparate Eurasian countries. The Monne et al. (2008) test was modified and successfully detected all H9 viruses from all three Eurasian H9 lineages. Bayesian analysis of the clinical specimens' results revealed this test to be more sensitive (97%) than the Ben Shabat et al. (2010) test (31%). The latter test detected most H9 isolates of the G1 lineage, but no isolates from other H9 lineages. Mismatches in the primer/probe binding sequences accounted for sensitivity differences between the two H9 RRT-PCRs. Genetic analysis of 34 sequenced H9 haemagglutinin genes showed the South Asian and Middle-East H9 isolates to belong to the H9 G1 lineage, and possessed residues that appear to preferably bind alpha 2,6-linked sialic acid receptors which indicate a potential for human infection. European H9s clustered phylogenetically in a broader geographical group that includes recent North American H9 wild bird isolates and contemporary Asian viruses in the Y439 H9 lineage

Protective effects of oral antioxidants on skin and eye function

Antioxidants applied topically and/or taken systemically may play an important role in counteracting the oxidative injury to lipids and proteins on the skin and eye that are provoked by radical oxygen species