7 research outputs found
Interphase cytogenetics and comparative genomic hybridization of human epithelial cancers and precursor lesions
Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization. EDTA is highly preferable to a routinely used acid decalcifier
Decalcification is routinely performed for histological studies of
bone-containing tissue. Although DNA in situ hybridization (ISH) and
comparative genomic hybridization (CGH) have been successfully employed on
archival material, little has been reported on the use of these techniques
on archival decalcified bony material. In this study we compared the
effects of two commonly used decalcifiers, i.e. , one proprietary,
acid-based agent (RDO) and one chelating agent (EDTA), in relation to
subsequent DNA ISH and CGH to bony tissues (two normal vertebrae, six
prostate tumor bone metastases with one sample decalcified by both EDTA
and RDO). We found that RDO-decalcified tissue was not suited for DNA ISH
in tissue sections with centromere-specific probes, whereas we were able
to adequately determine the chromosomal status of EDTA-decalcified
material of both control and tumor material. Gel electrophoresis revealed
that no DNA could be successfully retrieved from RDO-treated material.
Moreover, in contrast to RDO-decalcified tumor material, we detected
several chromosomal imbalances in the EDTA-decalcified tumor tissue by CGH
analysis. Furthermore, it was possible to determine the DNA ploidy status
of EDTA- but not of RDO-decalcified material by DNA flow cytometry.
Decalcification of bony samples by EDTA is highly recommended for
application in DNA ISH and CGH techniques
Interphase in situ hybridization to disaggregated and intact tissue specimens of prostatic adenocarcinomas
Molecular cytogenetic analysis of prostatic adenocarcinomas from screening studies : early cancers may contain aggressive genetic features
No objective parameters have been found so far that can predict the
biological behavior of early stages of prostatic cancer, which are
encountered frequently nowadays due to surveillance and screening
programs. We have applied comparative genomic hybridization to routinely
processed, paraffin-embedded radical prostatectomy specimens derived from
patients who participated in the European Randomized Study of Screening
for Prostate Cancer. We defined a panel consisting of 36 early cancer
specimens: 13 small (total tumor volume (Tv) < 0.5 ml) carcinomas and 23
intermediate (Tv between 0.5-1.0 ml) tumors. These samples were compared
with a set of 16 locally advanced, large (Tv > 2.0 ml) tumor samples, not
derived from the European Randomized Study of Screening for Prostate
Cancer. Chromosome arms that frequently (ie, > or = 15%) showed loss in
the small tumors included 13q (31%), 6q (23%), and Y (15%), whereas
frequent (ie, > or = 15%) gain was seen of 20q (15%). In the intermediate
cancers, loss was detected of 8p (35%), 16q (30%), 5q (26%), Y (22%), 6q,
and 18q (both 17%). No consistent gains were found i