SLAVONIAN-SRIJEM PODOLIC CATTLE

We considered the history of breeding, morphological feature characteristics and producing performances of the only one herd of Slavonian-Srijem Podolic Cattle in Croatia. Futhermore, we also exposed the breeding plan and methods to preservation of Slavonian-Srijem Podolic Cattle genom, and reserch program of their morphological (osteological), producing and specilly fiziological feature characteristics. In future, we will explore the blood groups, polymorphism of protein\u27s system enzimatic activities and the others parametars which are in close relations with course and intensity of the metabolic processes

INVESTIGATION ON THE EFFICIENCY OF THE SUPEROVULATION AND SUPEROVULATORY RESPONSE AND THE NUMBER OF RECOVERED EMBRYOS AND THE RESULTS OF THEIR TRANSFER IN COWS AND HEIFFERS

Istraživanja uspješnosti superovulacije i superovulacijskog odgovora te broja dobivenih zametaka kao i uspjeha transfera svježih i duboko zamrznutih i kasnije odmrznutih zametaka goveda izvodili smo u 35 plotkinja. Superovulacliju smo inducirali s pripravnicima SŽK (Folligonᴿ), odnosno FSH (Folltropinᴿ) u 27 plotkinja. Rezultate superovulacije i superovulacijski odgovor 7. dan poslije inducirane ovulacije i U.O. -a pratili smo rektalnom i ultrasonografskom pretragom jajnika (ALOHA ECHO CAMERA SSD-210, DX II s transrektalnom sondom od 5 MHz-a) te utvrdili 154 (ˉx = 5,70 ± 0,69) žutih tijela. U23 plotkinje isprali smo ukupno 15 zametaka, od kojih smo kao sposobne za transfer ocijenili 10 te smo jednoga odmah transferirali u odabranu primačicu. Ostalih 9 dubolo smo smrznuli pomoću aparata za smrzavanje zametaka (LE NICOOBAC MS 21). Nakon određenog vremena zametke smo odmrznuli i nakon ponovne ocjene 7 zametaka smo transferirali u 7 sinkroniziranih primačica. U 3 primačice pojavio se opet estrus 25. dan,u jedne 30. dan, a u 3 primačice 35. dana od prethodnog estrusa, dok smo u jedne 34. dan ultrazvučno utvrdili gravidnost.Investigations on the efficiency of the superovulation and superovulatory response and the number of recovered embryos and also the transfer results of fresh and cryopreserved/thawed bovine embryos were performed on 35 females. Superovulation was induced using PMSG (FolligonR) or FSH (FolltropinR) preparations in 27 females. The results of the superovulation and superovulatory respons on day 7 after induced ovulation and A.l. were followed up by rectal and ultrasonographic examinalion of the ovaries (ALOHA ECHO CAMERA SSD-210, DX ll with 5 MHz transrectal transducer) and 154 (ˉx = 5.70 ±0.69 ) corpora lutea were established. In 23 females a total of 15 embryos were received and 10 embryos were classified as transferable and then one embryo was instantly transferred into the selected recipient. The other 9 embryos were cryopreserved using freezing machine (LE NICOOBAC MS 21). After some time embryos were thawed and following classification 7 were transferred into 7 synchronized recipients. In 3 recipinets estrus reappeared after 25 days, in one after 30 days, and in 3 recipients 35 days after the previous estrus, whereas the pregrancy was ultrasonically established in one recipient on the 34th day