MESH-RELATED COMPLICATIONS AFTER IMPLANTATION OF SYNTHETIC MESHES USING TROCAR AND ANCHORING SYSTEMS

Aim. To reduce incidence of mesh-related complications by comparative analysis of the results of vaginal extraperitoneal mesh-vaginopexy with use of trocar and anchoring systems.Materials and methods. A retrospective analysis of outpatient and hospital charts of 160 patients was performed, who underwent operative treatment of genital prolapse by vaginal extraperitoneal vaginopexy with Prolift, Johnson & Johnson, USA, Pelvix (Lintex, RF), Elevate (AMS, USA) and the Calistar system (Promedon, Argentina). The duration of postoperative follow-up was 3 years. In the 1st group, the patients were operated on using trocar techniques (Prolift, Pelvix). The second group consisted of patients operated on with the use of anchor technologies using the Elevate and Calistar methods. The degree of prolapse of the genitals was determined by the POP-Q system. The index of the influence of genital prolapse on the quality of life (IVQV) was assessed using a validated PD-QL questionnaire. To verify the accuracy of the hypothesis, Chi-square was used. Differences were considered valid for p<0,05.Results. In group 1, the mean time of surgery was 98±26 min. The frequency of erosions of the vaginal mucosa was 10,9%, in 4,1% of cases, a partial excision of the implant was carried out. In 1,4% there was a wrinkling of the implant, accompanied by a pain relief syndrome, in connection with which the implant was partially excised. Chronic pelvic pain was noted in 11,3% of patients. Dyspareunia de novo was noted in 11,0% of patients. Infection of the implant was observed in 2,7%, the implant was removed. Implant protrusion into the bladder occurred in 1 patient, the implant was partially excised. The frequency of recurrence of prolapse of the genitals was 10,4%. In group 2, the mean time of surgery was 79±32 min. The frequency of erosions of the vaginal mucosa was 3.1%, in 1 case, partial excision of the implant was required. Dyspareunia de novo was noted in 6,1% of patients, in 1 of them the implant was partially excised. Chronic pelvic pain was noted in 3,1% of patients − the pain syndrome was treated conservatively. The frequency of recurrence of genital prolapse in the 2nd group was 9,8%.Conclusion. Surgical treatment of genital prolapse with the use of synthetic meshes is highly effective, with both trocar and anchoring systems in use. It is advisable to give preference to anchor technologies, given the lower frequency of meshrelated complications

SOMATIC AND REPRODUCTIVE HEALTH PATTERNS IN CHILDREN AND ADOLESCENTS FROM TOFALARIA

We present the data on physical development, somatic pathology, reproductive disorders in children and teenagers from Tofalaria. Medical documentation and reports were analyzed, specialized surveys of children and teenagers by pediatricians, endocrinologist, gynecologist were carried out. Microscopy, oncocytology, PCR for detection sexually transmitted diseases (chlamidia, mycoplasma papillomavirus 16,18) and colposcopy were done in all teenaged girls. Evaluated morbidity was high. In indigenous Tofalaria population we found significant differences in rates of skeletal and conjunctive tissue disorders (incorrect posture, scoliosis, flatfoot) as well as endocrine disorders and nutrition (incorrect puberty, diffuse nontoxic goiter, growth retardation, hypotrophy). Reproductive health of girls in Tofalaria is characterized by early start of sexual activity, high number of abortion and high STD level and cervix condition is characterized by high rate of columnar epithelium ectopia to an ectocervix with often combination with inflammation. We suppose this situation as a consequences of its reproductive behavior. We believe that the target, long term program of educational, prophylactic and treatment efforts is needed to be developed for preservation of children and teenagers health in Tofalaria

Study of fertility and cytogenetic variability in androgenic plants (R0 and R1) of alloplasmic introgression lines of common wheat

Anther culture is one of the methods to obtain DH lines of wheat. A limitation of this method can be cytogenetic instability in plants R0, leading to a decrease in fertility or sterility. In this study, we have investigated the fertility of R0, the fertility and cytogenetic variability of R1 in alloplasmatic introgression lines of common wheat in order to develop a cytogenetically stable DH lines with introgressions from different species. Lines 311/134, 311/FL, 311/IR with the cytoplasm from H. vulgare were studied. 311/134 carries the wheat-rye 1RS.1BL and wheatwheatgrass 7DL-7Ai translocations; 311/FL has the 1RS.1BL translocation and probably introgressions from A. glaucum; and 311/IR has the wheat-rye 1RS.1BL and wheat-Ae. speltoides T2B/2S#2 translocations. Green seedlings developed in anther culture for all lines. Differences between the lines in the ability for androgenesis and in the level of fertility in R0 and R1 have been revealed. Depressed androgenesis, low fertility and high aneuploidy were observed in 311/IR. It has been proposed that the reason for this is cytogenetic instability in gametes, which is caused by Gc genes located on T2B/2S#2. 63.3 % of 311/134 and 311/FL R1 plants that were grown from low seed-set R0 plants were aneuploids. Fertile R0 regenerant plants were identified that segregated in R1 for fertility and chromosome numbers. It has been demonstrated that DH lines are best developed from highfertility R1 plants with 2n = 42 irrespective of fertility in R0

Adaptation of diagnostics methods of cognitive disorders in children in the format of online testing

The aim of the study - to adapt the existing methods of neuropsychological diagnostics to be carried out in an online format.Цель исследования – адаптация существующих методов нейропсихологической диагностики для проведения

ОСТРЫЙ ЭПИГЛОТТИТ У МАЛЬЧИКА 3 ЛЕТ (КЛИНИЧЕСКОЕ НАБЛЮДЕНИЕ)

Аarticle describes the case of epiglottitis in a boy 3 years old, who was hospitalized  in a children's infectious disease department of city hospital of Izhevsk. He was hospitalized  in the department with a diagnosis of acute laryngotracheitis constrictive.The diagnosis  was clarified following an anesthetized laryngoscopy. Описан клинический случай эпиглоттита у мальчика 3 лет, находившегося на стационарном лечении в детском инфекционном отделении 7-ой Городской  клинической  больницы г. Ижевска. Госпитализирован в отделение с диагнозом: «острый стенозирующий ларинготрахеит». Диагноз был уточнен после проведенной поднаркозной ларингоскопии

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

p53 and its major E3 ligase Mdm2 are both ubiquitinated and targeted to the proteasome for degradation. Despite the importance of this in regulating the p53 pathway, little is known about the mechanisms of proteasomal recognition of ubiquitinated p53 and Mdm2. In this study, we show that knockdown of the proteasomal ubiquitin receptor S5a/PSMD4/Rpn10 inhibits p53 protein degradation and results in the accumulation of ubiquitinated p53. Overexpression of a dominant-negative deletion of S5a lacking its ubiquitin-interacting motifs (UIM)s, but which can be incorporated into the proteasome, also causes the stabilization of p53. Furthermore, small-interferring RNA (siRNA) rescue experiments confirm that the UIMs of S5a are required for the maintenance of low p53 levels. These observations indicate that S5a participates in the recognition of ubiquitinated p53 by the proteasome. In contrast, targeting S5a has no effect on the rate of degradation of Mdm2, indicating that proteasomal recognition of Mdm2 can be mediated by an S5a-independent pathway. S5a knockdown results in an increase in the transcriptional activity of p53. The selective stabilization of p53 and not Mdm2 provides a mechanism for p53 activation. Depletion of S5a causes a p53-dependent decrease in cell proliferation, demonstrating that p53 can have a dominant role in the response to targeting S5a. This study provides evidence for alternative pathways of proteasomal recognition of p53 and Mdm2. Differences in recognition by the proteasome could provide a means to modulate the relative stability of p53 and Mdm2 in response to cellular signals. In addition, they could be exploited for p53-activating therapies. This work shows that the degradation of proteins by the proteasome can be selectively dependent on S5a in human cells, and that this selectivity can extend to an E3 ubiquitin ligase and its substrate

СРЕДНИЕ ГОДОВЫЕ ЭФФЕКТИВНЫЕ ДОЗЫ ОБЛУЧЕНИЯ В 2017 ГОДУ ЖИТЕЛЕЙ НАСЕЛЕННЫХ ПУНКТОВ РОССИЙСКОЙ ФЕДЕРАЦИИ, ОТНЕСЕННЫХ К ЗОНАМ РАДИОАКТИВНОГО ЗАГРЯЗНЕНИЯ ВСЛЕДСТВИЕ КАТАСТРОФЫ НА ЧЕРНОБЫЛЬСКОЙ АЭС (ДЛЯ ЦЕЛЕЙ ЗОНИРОВАНИЯ НАСЕЛЕННЫХ ПУНКТОВ)

The Chernobyl accident in 1986 is one of the most large-scale radiation accidents in the world. It led to radioactive contamination of large areas in the European part of the Russian Federation and at the neighboring countries. Now, there are more than 4000 settlements with the total population of 1.5 million in the radioactively contaminated areas of the Russian Federation. The Bryansk region is the most intensely contaminated region. For example, the Krasnogorskiy district still has settlements with the level of soil contamination by cesium-137 exceeding 40 Ci/km2. The regions of Tula, Kaluga and Orel are also significantly affected. In addition to these four regions, there are ten more regions with the radioactively contaminated settlements. After the Chernobyl accident, the affected areas were divided into zones of radioactive contamination. The attribution of the settlements to a particular zone is determined by the level of soil contamination with 137Cs and by a value of the average annual effective dose that could be formed in the absence of: 1) active measures for radiation protection, and 2) self-limitation in consumption of the local food products. The main regulatory document on this issue is the Federal law № 1244-1 (dated May, 15 1991) “On the social protection of the citizens who have been exposed to radiation as a result of the accident at the Chernobyl nuclear power plant”. The law extends to the territories, where, since 1991: 1) the average annual effective dose for the population exceeds 1 mSv (the value of effective dose that could be formed in the absence of active radiation protection measures and self-limitation in consumption of the local food products); 2) soil surface contamination with cesium-137 exceeds 1 Ci/km2.The paper presents results of calculations of the average effective doses in 2017. The purpose was to use the dose values (SGED90) in zonation of contaminated territories. Therefore, the calculations have been done under the assumption that the doses were formed in the absence of active radiation protection measures and self-limitation in consumption of the local food products. The dose to population, rather than the density of radioactive contamination of soil by 137Cs, is the most objective characteristic of the actual radiation exposure to the residents of the contaminated areas.Одна из самых масштабных радиационных катастроф в мире – авария на Чернобыльской АЭС в 1986 г. – привела к радиоактивному загрязнению значительных территорий европейской части Российской Федерации и сопредельных государств. В настоящее время в зонах радиоактивного загрязнения в 14 субъектах Российской Федерации находится 3855 населенных пунктов, где проживают более 1,5 млн человек. Наиболее интенсивно загрязнена Брянская область: так, в Красногорском районе до сих пор есть населенные пункты с радиоактивным загрязнением почвы цезием-137 выше 50 Ки/км2. Значительно пострадали также Тульская, Калужская и Орловская области. Кроме этих четырех областей, еще в 10 регионах страны имеются населенные пункты, расположенные в зонах радиоактивного загрязнения. После аварии на ЧАЭС пострадавшие территории были разбиты на зоны радиоактивного загрязнения. Отнесение НП к той или иной зоне определяется уровнем загрязнения почвы 137Cs и величиной средней годовой эффективной дозы облучения, которую могли бы получить жители в условиях отсутствия активных мер радиационной защиты и самоограничений в потреблении местных пищевых продуктов. Основным нормативным документом по данному вопросу является Федеральный закон от 15 мая 1991 г. № 1244-1 «О социальной защите граждан, подвергшихся воздействию радиации вследствие катастрофы на Чернобыльской АЭС», распространяющийся на территории, на которых, начиная с 1991 г.:1) средняя годовая эффективная доза облучения населения превышает 1 мЗв/год – доза, которую могли бы получить жители в условиях отсутствия активных мер радиационной защиты и самоограничений в потреблении местных пищевых продуктов; 2) плотность радиоактивного загрязнения почвы цезием-137 превышает 1 Ки/км2.В статье приведены результаты расчетов средних годовых эффективных доз облучения населения в предполагаемых условиях отсутствия активных мер радиационной защиты, предназначенные для целей зонирования населенных пунктов (СГЭД90). Как известно, наиболее объективным качественным и количественным показателем оценки реального радиационного воздействия на жителей загрязненных территорий является доза облучения населения, а не плотность радиоактивного загрязнения почвы

ОБЛУЧЕНИЕ НАСЕЛЕНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ ВСЛЕДСТВИЕ АВАРИИ НА ЧЕРНОБЫЛЬСКОЙ АЭС И ОСНОВНЫЕ НАПРАВЛЕНИЯ ДАЛЬНЕЙШЕЙ РАБОТЫ НА ПРЕДСТОЯЩИЙ ПЕРИОД

The article discusses the issues associated with the current exposure of the population of 14 regions of Russia due to the Chernobyl accident, identified tasks facing Rospotrebnadzor today. The article describes criteria and requirements for ensuring of the mechanism of the transition of settlements from the conditions of a radiation accident to the public normal conditions.В статье рассматриваются вопросы, связанные с текущим облучением населения 14 регионов России вследствие аварии на Чернобыльской АЭС. Определены задачи, стоящие перед Роспотребнадзором в настоящее время. Описаны критерии и требования по обеспечению процедуры перехода населенных пунктов от условий радиационной аварии к условиям нормальной жизнедеятельности населения