2 research outputs found
Comparing antibody responses in chickens against Plasmodium falciparum lactate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase with Freundâs and PheroidÂź adjuvants
PheroidÂź technology was assessed as an alternative to Freundâs adjuvant to raise antibodies in experimental animals. Chickens were immunized with two recombinantly expressed Plasmodium falciparum proteins, lactate dehydrogenase (PfLDH) and glyceraldehyde-3-phosphate dehydrogenase (PfGAPDH), alone or in combination with Freundâs adjuvant or PheroidÂź. Chicken egg yolk antibodies (IgY) were isolated and compared for specificity, sensitivity and yield. Freundâs adjuvant and PheroidÂź stimulated prolonged antibody responses in chickens against both antigens. Affinity purified antibodies had specificity for the recombinant and the native proteins on Western blots. Antibodies generated in the presence of Freundâs adjuvant had high sensitivity for both antigens. PheroidÂź generated antibodies that detected the lowest concentration of recombinant PfLDH. Freundâs adjuvant and PheroidÂź both improved chicken IgY yields, with PheroidÂź showing a 2-fold increase relative to controls. PheroidÂź was well-tolerated in chickens and has potential for development as a safe adjuvant for testing alternative stimulatory factors to improve adjuvant formulation