Cell-based monitoring of cancer

This thesis aimed to optimize the predictive and prognostic information that can be obtained from Circulating Tumor cells (CTC) and Circulating Endothelial Cells (CEC) in whole blood by improving and standardization of their detection and characterization methods in patients with solid malignancies. Both CTC and CEC are promising biomarkers for research purpose and patient management, that can be easily obtained by a non-invasive blood sampling (i.e. liquid biopsy) on multiple time points

Calorons, Nahm's equations on S^1 and bundles over P^1xP^1

The moduli space of solutions to Nahm's equations of rank (k,k+j) on the circle, and hence, of SU(2) calorons of charge (k,j), is shown to be equivalent to the moduli of holomorphic rank 2 bundles on P^1xP^1 trivialized at infinity with c_2=k and equipped with a flag of degree j along P^1x{0}. An explicit matrix description of these spaces is given by a monad constructio

HI Bright Galaxies in the Southern Zone of Avoidance

A blind survey for HI bright galaxies in the southern Zone of Avoidance, (212 deg < l < 36 deg; |b| < 5 deg), has been made with the 21 cm multibeam receiver on the Parkes 64 m radiotelescope. The survey, sensitive to normal spiral galaxies to a distance of about 40 Mpc and more nearby dwarfs, detected 110 galaxies. Of these, 67 have no counterparts in the NASA/IPAC Extragalactic Database. In general, the uncataloged galaxies lie behind thicker obscuration than do the cataloged objects. All of the newly-discovered galaxies have HI flux integrals more than an order of magnitude lower than the Circinus galaxy. The survey recovers the Puppis cluster and foreground group (Kraan-Korteweg & Huchtmeier 1992), and the Local Void remains empty. The HI mass function derived for the sample is satisfactorily fit by a Schechter function with parameters alpha = 1.51 +- 0.12, Phi* = 0.006 +- 0.003, and log M* = 9.7 +- 0.10.Comment: To appear in The Astronomical Journa

Visible diode lasers can be used for flow cytometric immunofluorescence and DNA analysis

This report describes a feasibility study concerning the use of a visible diode laser for two important fluorescence applications in a flow cytometer. With a 3 mW 635 nm. diode laser, we performed immunofluorescence measurements using the fluorophore allophycocyanin (APC). We have measured CD8 positive lymphocytes with a two-step labeling procedure and the resulting histograms showed good separation between the negative cells and the dim and the bright fluorescent subpopulations. As a second fluorescence application, we chose DNA analysis with the recently developed DNA/ RNA stains TOTO-3 and TO-PRO-3. In our setup TO-PRO-3 yielded the best results with a CV of 3.4%. Our results indicate that a few milliwatts of 635 nm light from a visible diode laser is sufficient to do single color immunofluorescence measurements with allophycocyanin and DNA analysis with TO-PRO-3. The major advantages of using a diode laser in a flow cytometer are the small size, the low price, the high efficiency, and the long lifetime

Loop expansion in Yang-Mills thermodynamics

We argue that a selfconsistent spatial coarse-graining, which involves interacting (anti)calorons of unit topological charge modulus, implies that real-time loop expansions of thermodynamical quantities in the deconfining phase of SU(2) and SU(3) Yang-Mills thermodynamics are, modulo 1PI resummations, determined by a finite number of connected bubble diagrams.Comment: 15 pages, 2 figures, v5: discussion of much more severely constrained nonplanar situation included in Sec.