MONITORING VÝSKYTU PORÚCH OSOVÉHO ORGÁNU U ŠTUDENTOV DENTÁLNEJ HYGIENY

MONITORING THE OCCURRENCE OF THE AXIAL ORGAN DEFECTS IN DENTAL HYGIENE STUDENTS. Objective: The aim of the research was to evaluate changes in the curvature of the spine in the basic position in the sagittal and frontal plane in dental hygiene students using SpinalMouse, as well as comparing the quality of posture with a group of physiotherapy students. File: The surveyed sample consisted of 120 Presov University students, aged 21 to 34 years. Methods: Measurements were made using the SpinalMouse device. The percentage representation of changes in the basic shape of the spine in the sagittal and in the frontal plane in the various spine sections were calculated from the acquired data within the entire file. Results: The main problems were diagnosed in the frontal plane in the lumbar section of the spine. Pathological curvature without emphasis on the assessment of the curvature degree was found in more than 90% of the examined persons. Conclusion: The research highlights the negative changes in the parameters of the spinal shape in the examination sample of students. In order to prevent many health risks, attention should be paid to the method of teaching healthy habits within the work of dentists or dental hygienists

Strony obiektów opieki zdrowotnej i ich wpływ na zadowolenie klienta z perspektywy zarządzania relacjami klienta (CRM)

Recently, customer relationship management (CRM) applications have also shown importance in areas where demand exceeds supply. The core of CRM is based on marketing and communication with the customer is an important part of the marketing philosophy. The primary objective of this study is to highlight the importance of dental healthcare website elements from the customer's perspective in terms of customer satisfaction. The primary data included 448 respondents. The parameters of the website were assessed in the research, where it was found that there should be a term management element and a reminder system (e.g. SMS) on the dentist's website. In general, it can be said about linking the primary e-communication between the doctor and the patient, the doctor should primarily focus on inviting the patient to attend a medical examination or should provide information about preventive examinations. These issues have the greatest impact on overall satisfaction. These outputs were achieved using frequency and descriptive statistical characteristics and regulatory models (ridge, lasso, elastic net) and a multiple linear regression analysis model.Ostatnio aplikacje zarządzania relacjami z klientami (CRM) również mają znaczenie w obszarach, w których popyt przewyższa podaż. Podstawą CRM jest marketing i komunikacja z klientem, która jest ważną częścią filozofii marketingowej. Głównym celem tego badania jest podkreślenie znaczenia elementów strony internetowej opieki stomatologicznej z punktu widzenia klienta pod względem satysfakcji klienta. Podstawowe dane obejmowały 448 respondentów. Parametry strony internetowej zostały ocenione w badaniu, w którym stwierdzono, że na stronie internetowej dentysty powinien istnieć element zarządzania terminami i system przypomnień (np. SMS). Ogólnie rzecz biorąc, można powiedzieć o łączeniu podstawowej komunikacji elektronicznej między lekarzem a pacjentem, lekarz powinien przede wszystkim skupić się na zaproszeniu pacjenta do wzięcia udziału w badaniu lekarskim lub powinien dostarczyć informacji na temat badań profilaktycznych. Kwestie te mają największy wpływ na ogólne zadowolenie. Wyniki te uzyskano za pomocą częstotliwości i opisowych charakterystyk statystycznych oraz modeli regulacyjnych (grzbiet, lasso, siatka elastyczna) i wielorakiego modelu analizy regresji liniowej

Active site complementation and hexameric arrangement in the GH family 29; a structure–function study of α-l-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus

α-l-Fucosidase isoenzyme 1 from bacterium Paenibacillus thiaminolyticus is a member of the glycoside hydrolase family GH29 capable of cleaving l-fucose from nonreducing termini of oligosaccharides and glycoconjugates. Here we present the first crystal structure of this protein revealing a novel quaternary state within this family. The protein is in a unique hexameric assembly revealing the first observed case of active site complementation by a residue from an adjacent monomer in this family. Mutation of the complementing tryptophan residue caused changes in the catalytic properties including a shift of the pH optimum, a change of affinity to an artificial chromogenic substrate and a decreased reaction rate for a natural substrate. The wild-type enzyme was active on most of the tested naturally occurring oligosaccharides and capable of transglycosylation on a variety of acceptor molecules, including saccharides, alcohols or chromogenic substrates. Mutation of the complementing residue changed neither substrate specificity nor the preference for the type of transglycosylation acceptor molecule; however, the yields of the reactions were lower in both cases. Maltose molecules bound to the enzyme in the crystal structure identified surface carbohydrate-binding sites, possibly participating in binding of larger oligosaccharides

Salivary Extracellular DNA and DNase Activity in Periodontitis

Extracellular DNA (ecDNA) is a potential marker and predictor in several inflammatory diseases. Periodontitis, a chronic inflammatory disease, is associated with epithelial cell death and could lead to release of DNA. Our aim was to analyze salivary DNA concentration and deoxyribonuclease (DNase) activity in periodontitis patients. We hypothesized that salivary ecDNA will be higher than in controls and could serve as a marker of periodontitis severity. Samples of saliva were collected from 25 patients with chronic periodontitis and 29 age-matched controls. DNA was quantified fluorometrically in whole saliva, as well as in supernatants after centrifugation (depletion of cells at 1600× g) and in double-centrifuged supernatants (depletion of cell debris at 1600× g and 16,000× g). The subcellular origin of ecDNA was assessed using real-time PCR. In comparison to controls, patients with periodontitis had twofold higher salivary DNA (p < 0.01), higher mitochondrial DNA in centrifuged supernatants (p < 0.05) and lower nuclear ecDNA in double-centrifuged samples (p < 0.05). No correlations were found between salivary DNA and oral health status, but mitochondrial DNA positively correlated with papillary bleeding index in centrifuged samples. Salivary DNase activity was comparable between the groups. In conclusion, we proved that salivary DNA is higher in periodontitis. The source of the higher mitochondrial DNA in cell-free saliva and the causes of lower nuclear ecDNA remain to be elucidated. Further studies should focus on the role of mitochondrial DNA as a potential driver of inflammation in periodontitis