6 research outputs found
The influence of drought on the occurrence of aflatoxins in maize
In this study, a total of 78 maize samples harvested during September and October 2012 in Vojvodina were analyzed. Presence of aflatoxins (AFs) was determined by enzyme-linked immunosorbent assay (ELISA) method. Among the 78 analyzed maize samples, even 44 (56.4%) samples were contaminated with AFs. Concentration interval between 1-10 μg/kg, 10-50 μg/kg and 50-80 μg/kg were found in 23.1%, 17.9% and 15.4% of analyzed maize samples, respectively. It was supposed that prolonged drought during spring and summer of 2012 had a great influence on high contamination frequency and concentration of AFs. [Projekat Ministarstva nauke Republike Srbije, br. III 46001
PRESENCE OF AFLATOXINS IN CEREALS FROM SERBIA
ABSTRACT: Aflatoxins (AFs), one of the most toxic and the strongest natural carcinogens can be found in a variety of food commodities, including cereals. For that purpose, the aim of this study was to investigate occurrence of AFs (AFB1, AFG1, AFB2 and AFG2) in 130 cereal samples
Mycotoxins in maize: Annual variations and the impact of climate change
The presence of aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEA),
deoxynivalenol (DON), and fumonisins (FUMs) was examined in maize samples
from the Republic of Serbia. The maize samples were collected during the
period 2012-2016, and analyzed every year after harvest using validated
Enzyme Linked Immunosorbent Assay (ELISA) method. The obtained results were
considered regarding the weather conditions parameters recorded during the
investigated maize growing seasons. Significant differences in
weather-related parameters recorded in the five-year period resulted in
different mycotoxin profiles between the investigated years. Obtained
results indicate that the presence of ZEA and DON in maize is characteristic
of years with abundant precipitation, while AFs and OTA mainly occur in
maize during hot and dry years. Furthermore, FUMs were detected with
different contamination frequency in maize samples from every year. Based on
the findings obtained in this study, as well as on noted changes in weather
conditions in the recent years it could be assumed that maize from Serbia
may become susceptible to problems concerning mycotoxins. Therefore, there
is a necessity for monitoring and research related to the mycotoxins
occurrence in maize from Serbia
Direct chronopotentiometric analysis of riboflavin using a glassy carbon vessel as the working electrode
A new method for the determination of riboflavin (vitamin B2) was developed
based on chronopotentiometry with a glassy carbon process vessel
macroelectrode. The method optimisation included investigation of the most
important experimental parameters: type and concentration of the supporting
electrolyte, initial potential, reduction current, and the working electrode
surface area. The reduction signal of riboflavin appeared at about -0.12 V
vs. Ag/AgCl (3.5 mol/dm3 KCl) electrode in 0.025 mol/dm3 HCl as the
supporting electrolyte. A linear response was obtained in the the range of
0.05-4 mg/dm3. The limit of detection and limit of quantitation were 0.018
mg/dm3 and 0.054 mg/dm3, respectively. Due to the use of specific working
electrode, a significant enhancement of the method relative sensitivity of
about 10 times was achieved. The accuracy of the defined method was
confirmed by HPLC analyses. The developed method was successfully applied
for the quantitation of riboflavin in various pharmaceutical multivitamin
preparations. [Projekat Ministarstva nauke Republike Srbije, br. III 46009
Determination of multiple mycotoxins in maize using quechers sample preparation and LC-MS/MS detection
A reliable and easy method has been used for the multiple mycotoxins
determination of AFs, DON, ZEA and FBs in maize samples. Liquid
chromatography coupled mass spectrometry (LC-MS/MS) was used. Mycotoxins
have been extracted from maize using a QuEChERS-based extraction procedure.
All validation parameters were in accordance with Reg. (EC) No 401/2006.
The analyses of eight maize seed samples showed the AFs, DON, ZEA and FBs
contamination with the values below the state limit standards