The influence of drought on the occurrence of aflatoxins in maize

In this study, a total of 78 maize samples harvested during September and October 2012 in Vojvodina were analyzed. Presence of aflatoxins (AFs) was deter­mined by enzyme-linked immunosorbent assay (ELISA) method. Among the 78 analyzed maize samples, even 44 (56.4%) samples were contaminated with AFs. Concentration interval between 1-10 μg/kg, 10-50 μg/kg and 50-80 μg/kg were found in 23.1%, 17.9% and 15.4% of analyzed maize samples, respectively. It was supposed that prolonged drought during spring and summer of 2012 had a great influence on high contamination frequency and concentration of AFs. [Projekat Ministarstva nauke Republike Srbije, br. III 46001

PRESENCE OF AFLATOXINS IN CEREALS FROM SERBIA

ABSTRACT: Aflatoxins (AFs), one of the most toxic and the strongest natural carcinogens can be found in a variety of food commodities, including cereals. For that purpose, the aim of this study was to investigate occurrence of AFs (AFB1, AFG1, AFB2 and AFG2) in 130 cereal samples

Mycotoxins in maize: Annual variations and the impact of climate change

The presence of aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), and fumonisins (FUMs) was examined in maize samples from the Republic of Serbia. The maize samples were collected during the period 2012-2016, and analyzed every year after harvest using validated Enzyme Linked Immunosorbent Assay (ELISA) method. The obtained results were considered regarding the weather conditions parameters recorded during the investigated maize growing seasons. Significant differences in weather-related parameters recorded in the five-year period resulted in different mycotoxin profiles between the investigated years. Obtained results indicate that the presence of ZEA and DON in maize is characteristic of years with abundant precipitation, while AFs and OTA mainly occur in maize during hot and dry years. Furthermore, FUMs were detected with different contamination frequency in maize samples from every year. Based on the findings obtained in this study, as well as on noted changes in weather conditions in the recent years it could be assumed that maize from Serbia may become susceptible to problems concerning mycotoxins. Therefore, there is a necessity for monitoring and research related to the mycotoxins occurrence in maize from Serbia

Direct chronopotentiometric analysis of riboflavin using a glassy carbon vessel as the working electrode

A new method for the determination of riboflavin (vitamin B2) was developed based on chronopotentiometry with a glassy carbon process vessel macroelectrode. The method optimisation included investigation of the most important experimental parameters: type and concentration of the supporting electrolyte, initial potential, reduction current, and the working electrode surface area. The reduction signal of riboflavin appeared at about -0.12 V vs. Ag/AgCl (3.5 mol/dm3 KCl) electrode in 0.025 mol/dm3 HCl as the supporting electrolyte. A linear response was obtained in the the range of 0.05-4 mg/dm3. The limit of detection and limit of quantitation were 0.018 mg/dm3 and 0.054 mg/dm3, respectively. Due to the use of specific working electrode, a significant enhancement of the method relative sensitivity of about 10 times was achieved. The accuracy of the defined method was confirmed by HPLC analyses. The developed method was successfully applied for the quantitation of riboflavin in various pharmaceutical multivitamin preparations. [Projekat Ministarstva nauke Republike Srbije, br. III 46009

Determination of multiple mycotoxins in maize using quechers sample preparation and LC-MS/MS detection

A reliable and easy method has been used for the multiple mycotoxins determination of AFs, DON, ZEA and FBs in maize samples. Liquid chromatography cou­pled mass spectrometry (LC-MS/MS) was used. Mycotoxins have been extracted from maize using a QuEChERS-based extraction procedure. All validation parameters were in accordance with Reg. (EC) No 401/2006. The analyses of eight maize seed samples showed the AFs, DON, ZEA and FBs contamination with the values below the state limit standards