NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION

The technology of isolation of the cholera vibrio complex of low-molecular enzymes related to pathogenicity factors, from cholera vaccine production wastes by means of concentrating ultrafiltration was developed. This technology allows to obtain standard series of the preparation termed proteovibrin which is free from the basic Vibrio cholerae immunogen - O-antigen, and possesses high activity of protease and. phospholipases A2 and. C. It is shown that proteovibrin is highly soluble in water and 0.14 molar NaCl solution, causes the production of antibodies in rabbits and is toxic for white mice. Its further examination seems to be promising, as well as its possible use as additional source of the cholera vibrio enzymes and. as tripsin-like preparation, applicable for protein raw material hydrolysis in production of microbiological nutrient media

Scaling response of the amorphous alloy surface on loading and extrinsic crystallization

The surface nano-topography of the amorphous alloy Fe77Ni1Si9B13 was found to be anisotropic and fractal with the absolute values of Hurst exponent and fractal dimension close to those known for fracture surfaces of both conventional and amorphous metals. The tensile stress application causes the formation of shear bands (SBs), the highly prevailing orientation of which is normal to the stress applied. However, in very few cases, the SBs oriented at 45° to the stress direction detected. The latter SB orientation is typical for conventional (crystalline) metals where a specific dislocation mechanism determines the SBs orientation; the occurrence of 45°-oriented SBs in the amorphous alloy was attributed to the local, stress-induced extrinsic crystallization at the sample surface. In the region of heterogeneity of this kind, the scaling properties of surface relief were fully disturbed. The mechanism of the fractal roughness formation was related to the fractal geometry of thermal vibrations of walls of primary cavities during the new surface formation under critical conditions, such as that take place at fracturing or ultra high cooling