The Fluorescent Probing Method in the Estimation of the Functional Probabilities of the Blood Plasma Transport Systems

During this work method of fluorescent probes with differently charged fluorescent dyes was used for estimation of binding ability of the blood plasma main transport proteins. Investigations were done at the patients with liver cirrhosis for creating on those basis fluorescent tests adapted for clinical use. Such tests should use for depending of severity degree of pathological process and for the correct choice of the most effective haemosorbent for extracorporeal blood purification in the definite clinical situation. Regularity was shown for the changes in the binding ability of the blood plasma main transport proteins in liver cirrhosis, which depended on the stage of the disease and allowed efficiently estimate severity degree of pathological process. Comparative evaluation of the efficacy of different charcoal haemosorbents widely used in clinical practice as well as "Prometheus" device was done in terms of endogenous intoxication syndrome correction at liver cirrhosis patients using results of biochemical analysis and method of fluorescent dyes. It was shown that the most effective sorbents were sorbents of "Prometheus" and between charcoal heamosorbents - "Carbocol"

The Fluorescent Probing Method in the Estimation of the Functional Probabilities of the Blood Plasma Transport Systems

During this work method of fluorescent probes with differently charged fluorescent dyes was used for estimation of binding ability of the blood plasma main transport proteins. Investigations were done at the patients with liver cirrhosis for creating on those basis fluorescent tests adapted for clinical use. Such tests should use for depending of severity degree of pathological process and for the correct choice of the most effective haemosorbent for extracorporeal blood purification in the definite clinical situation. Regularity was shown for the changes in the binding ability of the blood plasma main transport proteins in liver cirrhosis, which depended on the stage of the disease and allowed efficiently estimate severity degree of pathological process. Comparative evaluation of the efficacy of different charcoal haemosorbents widely used in clinical practice as well as "Prometheus" device was done in terms of endogenous intoxication syndrome correction at liver cirrhosis patients using results of biochemical analysis and method of fluorescent dyes. It was shown that the most effective sorbents were sorbents of "Prometheus" and between charcoal heamosorbents - "Carbocol"

EVALUATION OF THE FUNCTIONAL PROBABILITIES OF THE BLOOD PLASMA TRANSPORT SYSTEMS OF THE LIVER CIRRHOSIS PATIENTS BY THE FLUORESCENT PROBES METHOD AND ASSESSING OF HYDROPHOBIC SUBSTANCES ELIMINATION AFTER HEMOSORPTION AT THESE PATIENTS

The results of investigation of the main blood plasma transport proteins binding ability at liver cirrhosis patients by the fluorescent probes method are presented. It is shown that fluorescent probes method allows to get express information about functional state of the main blood plasma transport proteins at liver cirrhosis patients, promptly assess the disease severity, and according to this information change their treatment quickly

Mucins in the mucosal barrier to infection

The mucosal tissues of the gastrointestinal, respiratory, reproductive, and urinary tracts, and the surface of the eye present an enormous surface area to the exterior environment. All of these tissues are covered with resident microbial flora, which vary considerably in composition and complexity. Mucosal tissues represent the site of infection or route of access for the majority of viruses, bacteria, yeast, protozoa, and multicellular parasites that cause human disease. Mucin glycoproteins are secreted in large quantities by mucosal epithelia, and cell surface mucins are a prominent feature of the apical glycocalyx of all mucosal epithelia. In this review, we highlight the central role played by mucins in accommodating the resident commensal flora and limiting infectious disease, interplay between underlying innate and adaptive immunity and mucins, and the strategies used by successful mucosal pathogens to subvert or avoid the mucin barrier, with a particular focus on bacteria

Band edge photoluminescence of undoped and doped TlInS2 layered crystals

Intrinsic photoluminescence (PL) of undoped and of B-, Ag- or Er-doped TlInS2 layered single crystals was investigated by confocal spectroscopy. It is found that position and intensity of PL spectral peak at 2.4 eV is vastly dependent on the excitation light incidence and polarization relative to crystallographic directions. For the normal incidence to the layer plane k||c, a significant Stokes shift between the PL peak in respect to exciton absorption energy is highlighted. The shift increases to 80 meV around the crystal phase transformations region of 200 K. We show that presence of B and Ag impurity do not diminish the intrinsic PL emission while Er atoms incorporation enhances it substantially and modifies a fine excitonic line structure in the ferroelectric phase at low T. The obtained results imply out-of-layer plain oriented 2D excitons in TlInS2 existing at various crystal phases which are influenced by related interlayer stresse

Опыт применения плазмообмена в интенсивной терапии миастенического криза и димиелинизирующих заболеваний

Одним из наиболее эффективных методов лечения при развитии критических состояний в неврологии является проведение плазмообмена. Мы кратко представляем анализ эффективности использования плазмообмена при тяжелом течении миастении гравис и димиелинизирующих заболеваний (ДМЗ) за последние 4 года в ведущих клиниках РБ. Экстракорпоральные операции проводились 116 пациентам с миастеническим кризом (МК), димиелинизирующими заболеваниями (ДМЗ), объективный статус оценивался по шкале APACHE III не менее 112,12 ± 4,10 баллов, вероятность летальности 75-80%.One of the most efficient treatment modes under development of critical states in neurology is plasmaexchange (PE) execution. Brief analysis of PE using efficiency at severe course of myasthenia gravis and demyelinating deseases (DMD) is represented based on results obtained for last 4 years in the our centers. PE were done for 116 patients with myasthenia crisis (MC), DMD and objective status estimated on APACHEIII scale has been no less than 112,12 ± 4,10 points, lethality probability – 75-80%

Band edge photoluminescence of undoped and doped TlInS2 layered crystals

Intrinsic photoluminescence (PL) of undoped and of B-, Ag- or Er-doped TlInS2 layered single crystals was investigated by confocal spectroscopy. It is found that position and intensity of PL spectral peak at 2.4 eV is vastly dependent on the excitation light incidence and polarization relative to crystallographic directions. For the normal incidence to the layer plane k||c, a significant Stokes shift between the PL peak in respect to exciton absorption energy is highlighted. The shift increases to 80 meV around the crystal phase transformations region of 200 K. We show that presence of B and Ag impurity do not diminish the intrinsic PL emission while Er atoms incorporation enhances it substantially and modifies a fine excitonic line structure in the ferroelectric phase at low T. The obtained results imply out-of-layer plain oriented 2D excitons in TlInS2 existing at various crystal phases which are influenced by related interlayer stresse

Photoelectrochemical and Raman characterization of In2O3 mesoporous films sensitized by CdS nanoparticles

The method of successive ion layer adsorption and reaction was applied for the deposition of CdS nanoparticles onto a mesoporous In2O3 substrate. The filling of the nanopores in In2O3 films with CdS particles mainly occurs during the first 30 cycles of the SILAR deposition. The surface modification of In2O3 with CdS nanoparticles leads to the spectral sensitization of photoelectrochemical processes that manifests itself in a red shift of the long-wavelength edge in the photocurrent spectrum by 100–150 nm. Quantum-confinement effects lead to an increase of the bandgap from 2.49 to 2.68 eV when decreasing the number of SILAR cycles from 30 to 10. The spectral shift and the widening of the Raman line belonging to CdS evidences the lattice stress on the CdS/In2O3 interfaces and confirms the formation of a close contact between the nanoparticles

Impact of intrinsic defects on excitation dependent carrier lifetime in thick 4H-SiC studied by complementing microwave photoconductivity, free-carrier absorption and time-resolved photoluminescence techniques

Abstract Photoexcited carrier dynamics in 1014 – 1018 cm-3 density range was investigated by using complementary optical and microwave techniques. Bulk lifetime decrease from 4 s to 460 ns with excitation and its increase with temperature were observed in bulk n-type 4H-SiC crystal. The latter data and modeling of excitation-dependent hole capture with subsequent electron capture provided the trap position at EV + 0.19 eV and electron (hole) lifetimes of 360 ns (100 ns), correspondingly, at high excitation conditions. Lifetime increase with temperature was observed due to the trap thermal activation and reduction of electron capture cross section. The trap origin was discussed in terms of silicon vacancy related and titanium point defects. Photoluminescence spectra in 24-300 K range revealed bound exciton luminescence and nitrogen-aluminum donor-acceptor pair band, which excitation dependent and time-resolved dynamics was analyzed in wide temperature range