61 research outputs found

    The Safety Culture As the Basis of the Digital Society

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    This article discusses the contradictions that emerge in global digital communication in a democracy and cultural diversity. The purpose of the study is to determine the dependence of a person’s goals on his \ her values in the dialogue of cultures and ways to solve the contradictions of intercultural communication. The social system does not only respond to the rapidly occurring social changes, but also respond to making political decisions. Russian scientist S.P. Kurdyumov expressed the idea that there are rules of the ban, the laws that limit the development. ’Safety Culture’ can be considered as the preservation and development of the ideals, values, norms and traditions of the individual, the family and society, social institutions and network. This study considers ’Safety Culture’ as value foundation for the digital society. This should be at the heart of every management decision referring to the digital future of our planet. A safety culture based on knowledge and observance of the ’prohibition rules’, the rules of behavior of people in global network communications will ensure the integrity of the world. Keywords: β€˜Safety Culture’, digital society, values, dialogue of culture

    Sustainability of the state system in the context of digital transformation

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    The digital transformation of the structural components of the state system forms new socio-organizational forms and practices of political interaction. The transformation process is based on the dialectical overcoming of existing elements of the old order, the development of new goals and the formation of new specific ways to achieve them. As a result of the digital transformation of the structural components of the state system, new socio-organizational forms and practices of political interaction are being formed. Before our eyes, the transition of initially networked social formations into political offline processes is unfolding, new power resources and mechanisms of influence on the political organization as a whole are emerging in society. These processes relate to social self-organization and are characteristic of the adaptive algorithm of social changes. At the same time, changes in system parameters do not violate the contours of the existing state system. However, in the conditions of deliberate stimulation by the authorities of the digital transformation of all spheres of society’s life, the limited ability of the political system and the state to ensure the manageability of social and political processes is more clearly manifested.The article considers the process of deformation of the state system caused by the digitalization of public relations, analyses changes in theoretical models of explaining social reality and related changes in the organization of the political system and political practices.The multi-component nature of the impact of the global ICT paradigm on the sphere of public administration of a particular state is noted. In the era of the diversity of broadcast information, the dominance of the mass media and social networks, the public becomes a mass / crowd and an object of manipulation. The impact of information and communication technologies on the functioning of society is manifested in maintaining the gap between formal and informal institutions, intensive introduction of innovations, launching new mechanisms of social inequality, and the like, as a result, the integrity of the social system is violated. The risk of loss of manageability of social processes increases due to the fact that the recommendations for authorities on digital transformation, as well as most scientific publications on the topic of digitalization of public administration do not contain a critical assessment of the ICT paradigm itself and the concepts, concepts and practices formed on its basis

    ΠΠžΠ’Π«Π™ ΠΠΠ‘ΠžΠ  Π Π•ΠΠ“Π•ΠΠ’ΠžΠ’ Π”Π›Π― Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― Π—Π•ΠΠ ΠΠ›Π•ΠΠžΠΠ Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    The EIA-ZEARALENONE reagent kit for the determination of zearalenone mycotoxin in feed and food by direct ELISA using microtitration plate has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of zearalenone content in agricultural products in a range of 50 to 800 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-Π—Π•ΠΠ ΠΠ›Π•ΠΠžΠ для опрСдСлСния микотоксина Π·Π΅Π°Ρ€Π°Π»Π΅Π½ΠΎΠ½Π° Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС Π·Π΅Π°Ρ€Π°Π»Π΅Π½ΠΎΠ½Π° Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 50 Π΄ΠΎ 800 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    ΠΠžΠ’Π«Π™ ΠΠΠ‘ΠžΠ  Π Π•ΠΠ“Π•ΠΠ’ΠžΠ’ Π”Π›Π― ИММ Π£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜ΠΠ Π’1 Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-AFLATOXIN for the determination of mycotoxin (aflatoxin B1) in feeds and foods by direct competitive enzyme immunoassay (EIA) has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the measurement technique correspond to the modern level of EIA development and provide the determination of aflatoxin B1 content in agricultural products in the range from 2.0 to 50.0 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜Π для опрСдСлСния микотоксина афлатоксина Π’1Β Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС афлатоксина Π’1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜Π для опрСдСлСния микотоксина афлатоксина Π’1Β Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС афлатоксина Π’1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    РЕАГЕНВЫ Π”Π›Π― Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― ВОКБИНА Π’-2 Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-TOXIN T-2 for the determination of mycotoxin T-2 toxin in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of T-2 toxin content of agricultural products in a range of 30 to 1000 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ВОКБИН Π’-2 для опрСдСлСния токсина Π’-2 Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС токсина Π’-2 Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 30 Π΄ΠΎ 1000 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ.Β 

    Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠ«Π™ ΠΠΠΠ›Π˜Π— Π€Π£ΠœΠžΠΠ˜Π—Π˜ΠΠžΠ’ Π“Π Π£ΠŸΠŸΠ« Π’ Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-FUMONISIN for the determination of mycotoxins of fumonisin B group in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated technicoanalytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the mo- dern level of immunoassay development and provide the determination of fumonisin group B content of agricultural products in a range of 0.11 to 6.0 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ ΠΏΡ€ΠΎΡˆΠ΅Π» Π²Π½ΡƒΡ‚Ρ€ΠΈΠ»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€Π½Ρ‹Π΅ испытания Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-Π€Π£ΠœΠžΠΠ˜Π—Π˜Π для опрСдСлСния микотоксинов, относящихся ΠΊ Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π°ΠΌ Π³Ρ€ΡƒΠΏΠΏΡ‹ Π’, Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ИЀА). Π’ состав Π½Π°Π±ΠΎΡ€Π° входят Ρ€Π°Π·Π±ΠΎΡ€Π½Ρ‹ΠΉ ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚, Π² Π»ΡƒΠ½ΠΊΠ°Ρ… ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ³ΠΎ биоспСцифичСски ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ моноклональноС Π°Π½Ρ‚ΠΈΡ‚Π΅Π»ΠΎ, Π³ΠΎΡ‚ΠΎΠ²Ρ‹ΠΉ ΠΊ использованию раствор ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚Π° Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π° Π’1 с пСроксидазой ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π°, Π³Ρ€Π°Π΄ΡƒΠΈΡ€ΠΎΠ²ΠΎΡ‡Π½Ρ‹Π΅ растворы, раствор Ρ…Ρ€ΠΎΠΌΠΎΠ³Π΅Π½Π° (Π’ΠœΠ‘), субстратный раствор (ΠΈΠ»ΠΈ Π³ΠΎΡ‚ΠΎΠ²Ρ‹ΠΉ ΠΊ использованию Ρ…Ρ€ΠΎΠΌΠΎΠ³Π΅Π½-субстратный раствор) ΠΈ стоп-Ρ€Π΅Π°Π³Π΅Π½Ρ‚. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ИЀА ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½ΠΎΠ² Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 0,11 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠΠ― Π‘Π˜Π‘Π’Π•ΠœΠ Π”Π›Π― ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― Π”Π•Π—ΠžΠšΠ‘Π˜ΠΠ˜Π’ΠΠ›Π•ΠΠžΠ›Π

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    A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² «ИЀА-Π”Π•Π—ΠžΠšΠ‘Π˜ΠΠ˜Π’ΠΠ›Π•ΠΠžΠ›Β» для опрСдСлСния дСзоксинивалСнола (Π”ΠžΠ) Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… для ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…, ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΈ ΠΏΡ€ΠΎΠ΄ΠΎΠ²ΠΎΠ»ΡŒΡΡ‚Π²Π΅Π½Π½ΠΎΠΌ ΡΡ‹Ρ€ΡŒΠ΅ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. Π‘Π°Π·ΠΎΠ²Ρ‹Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΏΡ€Π΅Π΄ΡΡ‚Π°Π²Π»ΡΡŽΡ‚ собой ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΎΠ½Π°Π»ΡŒΠ½Ρ‹Π΅ Π°Π½Ρ‚ΠΈΡ‚Π΅Π»Π° ΠΊ Π”ΠžΠ, ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Π΅ Π² Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ ΠΈΠΌΠΌΡƒΠ½ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΊΡ€ΠΎΠ»ΠΈΠΊΠΎΠ² ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ΠΎΠΌ Π”ΠžΠ с Π±Ρ‹Ρ‡ΡŒΠΈΠΌ сывороточным Π°Π»ΡŒΠ±ΡƒΠΌΠΈΠ½ΠΎΠΌ, ΠΈ ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ пСроксидазы ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π° с микотоксином. УстановлСны Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ. Набор позволяСт с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ Π”ΠžΠ Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΉ ΠΎΡ‚ 0,2 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³, ΠΏΡ€Π΅Π΄Π΅Π» количСствСнного опрСдСлСния исслСдуСмого микотоксина Π² Π·Π΅Ρ€Π½Π΅ ΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ… Π΅Π³ΠΎ ΠΏΠ΅Ρ€Π΅Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ Π½Π΅ ΠΏΡ€Π΅Π²Ρ‹ΡˆΠ°Π΅Ρ‚ 0,2 ΠΌΠ³/ΠΊΠ³

    ΠœΠ΅Ρ‚ΠΎΠ΄ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° для опрСдСлСния охратоксина Π° Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²Ρ‹Ρ… ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ…

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    A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by aΒ  directΒ  competitiveΒ  enzymeΒ  immunoassayΒ  usingΒ  microtitrationΒ  plateΒ  hasΒ  beenΒ  developedΒ  andΒ  tested.Β  TheΒ  conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² «ИЀА-ОΠ₯РАВОКБИН А» для опрСдСлСния охратоксина А Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. ΠšΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ охратоксина А с пСроксидазой ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π° ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½ с использованиСм Π°ΠΌΠΈΠ½ΠΎΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ микотоксина, присоСдинСнного ΠΊ ΡƒΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠΉ Ρ†Π΅ΠΏΠΈ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π°. МоноклональноС Π°Π½Ρ‚ΠΈΡ‚Π΅Π»ΠΎ ΠΊ опрСдСляСмому микотоксину ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ Π² Π»ΡƒΠ½ΠΊΠ°Ρ… ΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π° Ρ‡Π΅Ρ€Π΅Π· Π°Π½Ρ‚ΠΈΠ²ΠΈΠ΄ΠΎΠ²Ρ‹Π΅ Π°Π½Ρ‚ΠΈΡ‚Π΅Π»Π° Π±Π°Ρ€Π°Π½Π° ΠΊ ΠΈΠΌΠΌΡƒΠ½ΠΎΠ³Π»ΠΎΠ±ΡƒΠ»ΠΈΠ½Π°ΠΌ ΠΌΡ‹ΡˆΠΈ. УстановлСны Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° «ИЀА-ОΠ₯РАВОКБИН А» ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ. Набор позволяСт с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ охратоксин А Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ содСрТания ΠΎΡ‚ 5 Π΄ΠΎ 375 ΠΌΠΊΠ³/ΠΊΠ³, ΠΏΡ€Π΅Π΄Π΅Π» количСствСнного опрСдСлСния микотоксина Π² Π·Π΅Ρ€Π½Π΅ ΠΈ Π² ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ… Π΅Π³ΠΎ ΠΏΠ΅Ρ€Π΅Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ составляСт 5 ΠΌΠΊΠ³/ΠΊΠ³

    Drift theory of charged-particle motion for a strong electric field in a weakly relativistic approximation

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    Drift equations of motion are derived for a charged particle in the case of a strong electric field with allowance for relativistic effects of order v2/c2. The role of these effects is discussed along with the effects of a high-frequency field. The cases of weak and strong electric fields are distinguished [2] in the drift theory of the motion of charged particles in weakly inhomogeneous magnetic and electric fields. In the case of a weak electric field, the electric-drift velocity is vE β‰ͺ v, where v is the characteristic velocity of the particle. For a strong electric field, vE∼v. The drift theory has now been reasonably well developed for the case of weak electric fields in the classical and relativistic cases, for the absence of high-frequency fields and for the presence of these [1-3], Extension of the theory to strong electric fields involves considerable mathematical difficulties, and this has been done only in the classical approximation with and without hf fields [2-4], Here we consider the drift theory of charged-particle motion for the case of a strong electric field in the weakly relativistic approximation, incorporating terms of order v2/c2, where c is the velocity of light. Also hf fields may be present. Β© 1982 Plenum Publishing Corporation
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