61 research outputs found
The Safety Culture As the Basis of the Digital Society
This article discusses the contradictions that emerge in global digital communication in a democracy and cultural diversity. The purpose of the study is to determine the dependence of a personβs goals on his \ her values in the dialogue of cultures and ways to solve the contradictions of intercultural communication. The social system does not only respond to the rapidly occurring social changes, but also respond to making political decisions. Russian scientist S.P. Kurdyumov expressed the idea that there are rules of the ban, the laws that limit the development. βSafety Cultureβ can be considered as the preservation and development of the ideals, values, norms and traditions of the individual, the family and society, social institutions and network. This study considers βSafety Cultureβ as value foundation for the digital society. This should be at the heart of every management decision referring to the digital future of our planet. A safety culture based on knowledge and observance of the βprohibition rulesβ, the rules of behavior of people in global network communications will ensure the integrity of the world.
Keywords: βSafety Cultureβ, digital society, values, dialogue of culture
Sustainability of the state system in the context of digital transformation
The digital transformation of the structural components of the state system forms new socio-organizational forms and practices of political interaction. The transformation process is based on the dialectical overcoming of existing elements of the old order, the development of new goals and the formation of new specific ways to achieve them. As a result of the digital transformation of the structural components of the state system, new socio-organizational forms and practices of political interaction are being formed. Before our eyes, the transition of initially networked social formations into political offline processes is unfolding, new power resources and mechanisms of influence on the political organization as a whole are emerging in society. These processes relate to social self-organization and are characteristic of the adaptive algorithm of social changes. At the same time, changes in system parameters do not violate the contours of the existing state system. However, in the conditions of deliberate stimulation by the authorities of the digital transformation of all spheres of societyβs life, the limited ability of the political system and the state to ensure the manageability of social and political processes is more clearly manifested.The article considers the process of deformation of the state system caused by the digitalization of public relations, analyses changes in theoretical models of explaining social reality and related changes in the organization of the political system and political practices.The multi-component nature of the impact of the global ICT paradigm on the sphere of public administration of a particular state is noted. In the era of the diversity of broadcast information, the dominance of the mass media and social networks, the public becomes a mass / crowd and an object of manipulation. The impact of information and communication technologies on the functioning of society is manifested in maintaining the gap between formal and informal institutions, intensive introduction of innovations, launching new mechanisms of social inequality, and the like, as a result, the integrity of the social system is violated. The risk of loss of manageability of social processes increases due to the fact that the recommendations for authorities on digital transformation, as well as most scientific publications on the topic of digitalization of public administration do not contain a critical assessment of the ICT paradigm itself and the concepts, concepts and practices formed on its basis
ΠΠΠΠ«Π ΠΠΠΠΠ Π ΠΠΠΠΠΠ’ΠΠ ΠΠΠ― ΠΠΠΠ£ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠΠΠ ΠΠΠ ΠΠΠΠΠΠΠΠ― ΠΠΠΠ ΠΠΠΠΠΠΠ Π ΠΠΠ ΠΠΠ₯ Π ΠΠΠ©ΠΠΠ«Π₯ ΠΠ ΠΠΠ£ΠΠ’ΠΠ₯
The EIA-ZEARALENONE reagent kit for the determination of zearalenone mycotoxin in feed and food by direct ELISA using microtitration plate has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of zearalenone content in agricultural products in a range of 50 to 800 mg/kg with proper accuracy and precision.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΠΠ€Π-ΠΠΠΠ ΠΠΠΠΠΠ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π° Π·Π΅Π°ΡΠ°Π»Π΅Π½ΠΎΠ½Π° Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π½Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π·Π΅Π°ΡΠ°Π»Π΅Π½ΠΎΠ½Π° Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ 50 Π΄ΠΎ 800 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡΡΠΊΠΎΡ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ
ΠΠΠΠ«Π ΠΠΠΠΠ Π ΠΠΠΠΠΠ’ΠΠ ΠΠΠ― ΠΠΠ Π£ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠΠΠ ΠΠΠ ΠΠΠΠΠΠΠΠ― ΠΠ€ΠΠΠ’ΠΠΠ‘ΠΠΠ Π1 Π ΠΠΠ ΠΠΠ₯ Π ΠΠΠ©ΠΠΠ«Π₯ ΠΠ ΠΠΠ£ΠΠ’ΠΠ₯
A reagent kit EIA-AFLATOXIN for the determination of mycotoxin (aflatoxin B1) in feeds and foods by direct competitive enzyme immunoassay (EIA) has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the measurement technique correspond to the modern level of EIA development and provide the determination of aflatoxin B1 content in agricultural products in the range from 2.0 to 50.0 mg/kg with proper accuracy and precision.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΠΠ€Π-ΠΠ€ΠΠΠ’ΠΠΠ‘ΠΠ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π° Π°ΡΠ»Π°ΡΠΎΠΊΡΠΈΠ½Π° Π1Β Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π½Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π°ΡΠ»Π°ΡΠΎΠΊΡΠΈΠ½Π° Π1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡΡΠΊΠΎΡ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΠΠ€Π-ΠΠ€ΠΠΠ’ΠΠΠ‘ΠΠ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π° Π°ΡΠ»Π°ΡΠΎΠΊΡΠΈΠ½Π° Π1Β Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π½Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π°ΡΠ»Π°ΡΠΎΠΊΡΠΈΠ½Π° Π1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡΡΠΊΠΎΡ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ
Π ΠΠΠΠΠΠ’Π« ΠΠΠ― ΠΠΠΠ£ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠΠΠ ΠΠΠ ΠΠΠΠΠΠΠΠ― Π’ΠΠΠ‘ΠΠΠ Π’-2 Π ΠΠΠ ΠΠΠ₯ Π ΠΠΠ©ΠΠΠ«Π₯ ΠΠ ΠΠΠ£ΠΠ’ΠΠ₯
A reagent kit EIA-TOXIN T-2 for the determination of mycotoxin T-2 toxin in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of T-2 toxin content of agricultural products in a range of 30 to 1000 mg/kg with proper accuracy and precision.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΠΠ€Π-Π’ΠΠΠ‘ΠΠ Π’-2 Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΎΠΊΡΠΈΠ½Π° Π’-2 Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π½Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠΎΠΊΡΠΈΠ½Π° Π’-2 Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ 30 Π΄ΠΎ 1000 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡΡΠΊΠΎΡ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ.Β
ΠΠΠΠ£ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠ«Π ΠΠΠΠΠΠ Π€Π£ΠΠΠΠΠΠΠΠΠ ΠΠ Π£ΠΠΠ« Π Π ΠΠΠ ΠΠΠ₯ Π ΠΠΠ©ΠΠΠ«Π₯ ΠΠ ΠΠΠ£ΠΠ’ΠΠ₯
A reagent kit EIA-FUMONISIN for the determination of mycotoxins of fumonisin B group in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated technicoanalytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the mo- dern level of immunoassay development and provide the determination of fumonisin group B content of agricultural products in a range of 0.11 to 6.0 mg/kg with proper accuracy and precision.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΏΡΠΎΡΠ΅Π» Π²Π½ΡΡΡΠΈΠ»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠ΅ ΠΈΡΠΏΡΡΠ°Π½ΠΈΡ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΠΠ€Π-Π€Π£ΠΠΠΠΠΠΠ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ², ΠΎΡΠ½ΠΎΡΡΡΠΈΡ
ΡΡ ΠΊ ΡΡΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π°ΠΌ Π³ΡΡΠΏΠΏΡ Π, Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ΠΠ€Π). Π ΡΠΎΡΡΠ°Π² Π½Π°Π±ΠΎΡΠ° Π²Ρ
ΠΎΠ΄ΡΡ ΡΠ°Π·Π±ΠΎΡΠ½ΡΠΉ ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅Ρ, Π² Π»ΡΠ½ΠΊΠ°Ρ
ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ Π°Π½ΡΠΈΡΠ΅Π»ΠΎ, Π³ΠΎΡΠΎΠ²ΡΠΉ ΠΊ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΠ°ΡΡΠ²ΠΎΡ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠ° ΡΡΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π° Π1 Ρ ΠΏΠ΅ΡΠΎΠΊΡΠΈΠ΄Π°Π·ΠΎΠΉ ΠΈΠ· ΠΊΠΎΡΠ½Π΅ΠΉ Ρ
ΡΠ΅Π½Π°, Π³ΡΠ°Π΄ΡΠΈΡΠΎΠ²ΠΎΡΠ½ΡΠ΅ ΡΠ°ΡΡΠ²ΠΎΡΡ, ΡΠ°ΡΡΠ²ΠΎΡ Ρ
ΡΠΎΠΌΠΎΠ³Π΅Π½Π° (Π’ΠΠ), ΡΡΠ±ΡΡΡΠ°ΡΠ½ΡΠΉ ΡΠ°ΡΡΠ²ΠΎΡ (ΠΈΠ»ΠΈ Π³ΠΎΡΠΎΠ²ΡΠΉ ΠΊ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Ρ
ΡΠΎΠΌΠΎΠ³Π΅Π½-ΡΡΠ±ΡΡΡΠ°ΡΠ½ΡΠΉ ΡΠ°ΡΡΠ²ΠΎΡ) ΠΈ ΡΡΠΎΠΏ-ΡΠ΅Π°Π³Π΅Π½Ρ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΌΡ ΡΡΠΎΠ²Π½Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΠ€Π ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΡΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½ΠΎΠ² Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ 0,11 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡΡΠΊΠΎΡ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ
ΠΠΠΠ£ΠΠΠ€ΠΠ ΠΠΠΠ’ΠΠΠ― Π‘ΠΠ‘Π’ΠΠΠ ΠΠΠ― ΠΠΠ ΠΠΠΠΠΠΠΠ― ΠΠΠΠΠΠ‘ΠΠΠΠΠΠΠΠΠΠΠ
A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² Β«ΠΠ€Π-ΠΠΠΠΠΠ‘ΠΠΠΠΠΠΠΠΠΠΒ» Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π΄Π΅Π·ΠΎΠΊΡΠΈΠ½ΠΈΠ²Π°Π»Π΅Π½ΠΎΠ»Π° (ΠΠΠ) Π² ΠΊΠΎΡΠΌΠ°Ρ
Π΄Π»Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΈ ΠΏΡΠΎΠ΄ΠΎΠ²ΠΎΠ»ΡΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΡΡΡΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. ΠΠ°Π·ΠΎΠ²ΡΠ΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ Π½Π°Π±ΠΎΡΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ Π°Π½ΡΠΈΡΠ΅Π»Π° ΠΊ ΠΠΠ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΈΠΌΠΌΡΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ² ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠΌ ΠΠΠ Ρ Π±ΡΡΡΠΈΠΌ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΡΠΌ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠΌ, ΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°Ρ ΠΏΠ΅ΡΠΎΠΊΡΠΈΠ΄Π°Π·Ρ ΠΈΠ· ΠΊΠΎΡΠ½Π΅ΠΉ Ρ
ΡΠ΅Π½Π° Ρ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠΌ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ. ΠΠ°Π±ΠΎΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΠΠΠ Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΠΎΡ 0,2 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³, ΠΏΡΠ΅Π΄Π΅Π» ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠ³ΠΎ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π° Π² Π·Π΅ΡΠ½Π΅ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°Ρ
Π΅Π³ΠΎ ΠΏΠ΅ΡΠ΅ΡΠ°Π±ΠΎΡΠΊΠΈ Π½Π΅ ΠΏΡΠ΅Π²ΡΡΠ°Π΅Ρ 0,2 ΠΌΠ³/ΠΊΠ³
ΠΠ΅ΡΠΎΠ΄ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΎΡ ΡΠ°ΡΠΎΠΊΡΠΈΠ½Π° Π° Π² ΠΊΠΎΡΠΌΠ°Ρ ΠΈ ΠΏΠΈΡΠ΅Π²ΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°Ρ
A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by aΒ directΒ competitiveΒ enzymeΒ immunoassayΒ usingΒ microtitrationΒ plateΒ hasΒ beenΒ developedΒ andΒ tested.Β TheΒ conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΈΡΠΏΡΡΠ°Π½ Π½Π°Π±ΠΎΡ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² Β«ΠΠ€Π-ΠΠ₯Π ΠΠ’ΠΠΠ‘ΠΠ ΠΒ» Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΎΡ
ΡΠ°ΡΠΎΠΊΡΠΈΠ½Π° Π Π² ΠΊΠΎΡΠΌΠ°Ρ
ΠΈ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡΠΎΠΏΠ»Π°Π½ΡΠ΅ΡΠ½ΠΎΠΌ ΡΠΎΡΠΌΠ°ΡΠ΅. ΠΠΎΠ½ΡΡΠ³Π°Ρ ΠΎΡ
ΡΠ°ΡΠΎΠΊΡΠΈΠ½Π° Π Ρ ΠΏΠ΅ΡΠΎΠΊΡΠΈΠ΄Π°Π·ΠΎΠΉ ΠΈΠ· ΠΊΠΎΡΠ½Π΅ΠΉ Ρ
ΡΠ΅Π½Π° ΠΏΠΎΠ»ΡΡΠ΅Π½ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΌΠΈΠ½ΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π°, ΠΏΡΠΈΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½Π½ΠΎΠ³ΠΎ ΠΊ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠΉ ΡΠ΅ΠΏΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. ΠΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ Π°Π½ΡΠΈΡΠ΅Π»ΠΎ ΠΊ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΠΎΠΌΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Ρ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ Π² Π»ΡΠ½ΠΊΠ°Ρ
ΠΏΠ»Π°Π½ΡΠ΅ΡΠ° ΡΠ΅ΡΠ΅Π· Π°Π½ΡΠΈΠ²ΠΈΠ΄ΠΎΠ²ΡΠ΅ Π°Π½ΡΠΈΡΠ΅Π»Π° Π±Π°ΡΠ°Π½Π° ΠΊ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π°ΠΌ ΠΌΡΡΠΈ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΡΠ΅Ρ
Π½ΠΈΠΊΠΎ-Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π½Π°Π±ΠΎΡΠ° Β«ΠΠ€Π-ΠΠ₯Π ΠΠ’ΠΠΠ‘ΠΠ ΠΒ» ΠΈ ΠΌΠ΅ΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ. ΠΠ°Π±ΠΎΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Ρ Π½Π°Π΄Π»Π΅ΠΆΠ°ΡΠ΅ΠΉ ΡΠΎΡΠ½ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΠΎΡ
ΡΠ°ΡΠΎΠΊΡΠΈΠ½ Π Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΎΡ 5 Π΄ΠΎ 375 ΠΌΠΊΠ³/ΠΊΠ³, ΠΏΡΠ΅Π΄Π΅Π» ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠ½Π° Π² Π·Π΅ΡΠ½Π΅ ΠΈ Π² ΠΏΡΠΎΠ΄ΡΠΊΡΠ°Ρ
Π΅Π³ΠΎ ΠΏΠ΅ΡΠ΅ΡΠ°Π±ΠΎΡΠΊΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 5 ΠΌΠΊΠ³/ΠΊΠ³
Drift theory of charged-particle motion for a strong electric field in a weakly relativistic approximation
Drift equations of motion are derived for a charged particle in the case of a strong electric field with allowance for relativistic effects of order v2/c2. The role of these effects is discussed along with the effects of a high-frequency field. The cases of weak and strong electric fields are distinguished [2] in the drift theory of the motion of charged particles in weakly inhomogeneous magnetic and electric fields. In the case of a weak electric field, the electric-drift velocity is vE βͺ v, where v is the characteristic velocity of the particle. For a strong electric field, vEβΌv. The drift theory has now been reasonably well developed for the case of weak electric fields in the classical and relativistic cases, for the absence of high-frequency fields and for the presence of these [1-3], Extension of the theory to strong electric fields involves considerable mathematical difficulties, and this has been done only in the classical approximation with and without hf fields [2-4], Here we consider the drift theory of charged-particle motion for the case of a strong electric field in the weakly relativistic approximation, incorporating terms of order v2/c2, where c is the velocity of light. Also hf fields may be present. Β© 1982 Plenum Publishing Corporation
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