Process for preparation of large-particle-size monodisperse latexes

Monodisperse latexes having a particle size in the range of 2 to 40 microns are prepared by seeded emulsion polymerization in microgravity. A reaction mixture containing smaller monodisperse latex seed particles, predetermined amounts of monomer, emulsifier, initiator, inhibitor and water is placed in a microgravity environment, and polymerization is initiated by heating. The reaction is allowed to continue until the seed particles grow to a predetermined size, and the resulting enlarged particles are then recovered. A plurality of particle-growing steps can be used to reach larger sizes within the stated range, with enlarge particles from the previous steps being used as seed particles for the succeeding steps. Microgravity enables preparation of particles in the stated size range by avoiding gravity related problems of creaming and settling, and flocculation induced by mechanical shear that have precluded their preparation in a normal gravity environment

A Framework for Developing Portfolios of Improvements Projects in Manufacturing

AbstractThe outcome of improvement programmes such as Lean Manufacturing or Six Sigma is only partially determined by the success or failure of its individual projects. Also of significance is how well the programme and its projects are aligned to the company's strategy. Frequently practitioners will select projects on their individual merits, rather than with proper reference to their contribution to business strategy. In this manner, it is therefore possible to build portfolios of projects that are at best suboptimal and at worst counter to the company's overall strategic direction. The construction of project portfolios is thus a critical step in effective programme management and this would suggest that organizations would benefit from a framework to assist them with the selection of projects and portfolios that are aligned with the company's overall strategy. While tools such as Critical to Quality Flow-down are available to translate the voice of the customer to metrics and goals, practitioners do not have a structured approach to construct and assess portfolios.In this paper we present a framework to assist programme managers to develop portfolios of improvement projects targeted to fulfil their company's strategic needs and also align with the organisation's objectives and measures. Consideration is given to quantitative and qualitative aspects of strategy and how these may best be related to provide a set of orthogonal and common metrics

Effects of geometric anisotropy on local field distribution: Ewald-Kornfeld formulation

We have applied the Ewald-Kornfeld formulation to a tetragonal lattice of point dipoles, in an attempt to examine the effects of geometric anisotropy on the local field distribution. The various problems encountered in the computation of the conditionally convergent summation of the near field are addressed and the methods of overcoming them are discussed. The results show that the geometric anisotropy has a significant impact on the local field distribution. The change in the local field can lead to a generalized Clausius-Mossotti equation for the anisotropic case.Comment: Accepted for publications, Journal of Physics: Condensed Matte

The Impact of GPS Velocity Based Flight Control on Flight Instrumentation Architecture

This thesis explores the use of velocity information obtained by a Global Positioning System (GPS) receiver to close the aircraft’s flight control loop. A novel framework to synthesize attitude information from GPS velocity vector measurements is discussed. The framework combines the benefits of high-quality GPS velocity measurements with a novel velocity vector based flight control paradigm to provide a means for the human operator or autopilot to close the aircraft flight control loop. Issues arising from limitations in GPS as well as the presence of a human in the aircraft control loop are addressed. Results from several flight tests demonstrate the viability of this novel concept and show that GPS velocity based attitude allows for equivalent aircraft control as traditional attitude. Two possible applications of GPS velocity based attitude, an autopilot and a tunnelin- the-sky trajectory guidance system, are demonstrated in flight. Unlike traditional autopilot and trajectory guidance systems, these applications rely solely on the information obtained from a single-antenna GPS receiver which makes them affordable to the larger General Aviation aircraft community. Finally, the impact of GPS velocity based flight control on the instrumentation architecture of flight vehicles is investigated.Rockwell-Collins, NASA/FAA Joint University Program for Air Transportation, Draper Laborator

The measurement and analysis of age-related changes in Caenorhabditis elegans

Aging is characterized by progressive degenerative changes in tissue organization and function that increase the probability of mortality. Major goals of aging research include elucidating the series of events that cause degenerative changes and analyzing environmental and genetic factors that modulate these changes. The basis for mechanistic studies of aging are accurate and precise descriptions of age-related changes, since these descriptions define the aging phenotype. Here we review studies that describe age-related changes in C. elegans including measurements of integrated functions such as behavior, microscopic analyses of tissue organization, and biochemical studies of macromolecules. Genetic and environmental factors that influence these changes are described, and studies that analyze the relationships between different age-related changes are discussed. Together these studies provide fundamental insights into aging in C. elegans that may be relevant to aging in other animals

Mammalian GGAs act together to sort mannose 6-phosphate receptors

The GGAs (Golgi-localized, γ ear–containing, ADP ribosylation factor–binding proteins) are multidomain proteins implicated in protein trafficking between the Golgi and endosomes. We examined whether the three mammalian GGAs act independently or together to mediate their functions. Using cryo-immunogold electron microscopy, the three GGAs were shown to colocalize within coated buds and vesicles at the trans-Golgi network (TGN) of HeLa cells. In vitro binding experiments revealed multidomain interactions between the GGAs, and chemical cross-linking experiments demonstrated that GGAs 1 and 2 form a complex on Golgi membranes. RNA interference of each GGA resulted in decreased levels of the other GGAs and their redistribution from the TGN to cytosol. This was associated with impaired incorporation of the cation-independent mannose 6-phosphate receptor into clathrin-coated vesicles at the TGN, partial redistribution of the receptor to endosomes, and missorting of cathepsin D. The morphology of the TGN was also altered. These findings indicate that the three mammalian GGAs cooperate to sort cargo and are required for maintenance of TGN structure

The first products made in space: Monodisperse latex particles

The preparation of large particle size 3 to 30 micrometer monodisperse latexes in space confirmed that original rationale unequivocally. The flight polymerizations formed negligible amounts of coagulum as compared to increasing amounts for the ground-based polymerizations. The number of offsize large particles in the flight latexes was smaller than in the ground-based latexes. The particle size distribution broadened and more larger offsize particles were formed when the polymerizations of the partially converted STS-4 latexes were completed on Earth. Polymerization in space also showed other unanticipated advantages. The flight latexes had narrower particle size distributions than the ground-based latexes. The particles of the flight latexes were more perfect spheres than those of the ground-based latexes. The superior uniformity of the flight latexes was confirmed by the National Bureau of Standards acceptance of the 10 micrometer STS-6 latex and the 30 micrometer STS-11 latexes as Standard Reference Materials, the first products made in space for sale on Earth. The polymerization rates in space were the same as those on Earth within experimental error. Further development of the ground-based polymerization recipes gave monodisperse particles as large as 100 micrometer with tolerable levels of coagulum, but their uniformity was significantly poorer than the flight latexes. Careful control of the polymerization parameters gave uniform nonspherical particles: symmetrical and asymmetrical doublets, ellipsoids, egg-shaped, ice cream cone-shaped, and popcorn-shaped particles

Learning cellular morphology with neural networks

Reconstruction and annotation of volume electron microscopy data sets of brain tissue is challenging but can reveal invaluable information about neuronal circuits. Significant progress has recently been made in automated neuron reconstruction as well as automated detection of synapses. However, methods for automating the morphological analysis of nanometer-resolution reconstructions are less established, despite the diversity of possible applications. Here, we introduce cellular morphology neural networks (CMNs), based on multi-view projections sampled from automatically reconstructed cellular fragments of arbitrary size and shape. Using unsupervised training, we infer morphology embeddings (Neuron2vec) of neuron reconstructions and train CMNs to identify glia cells in a supervised classification paradigm, which are then used to resolve neuron reconstruction errors. Finally, we demonstrate that CMNs can be used to identify subcellular compartments and the cell types of neuron reconstructions

Zinc homeostasis and signaling in the roundworm C. elegans

C. elegans is a powerful model for studies of zinc biology. Here we review recent discoveries and emphasize the advantages of this model organism. Methods for manipulating and measuring zinc levels have been developed in or adapted to the worm. The C. elegans genome encodes highly conserved zinc transporters, and their expression and function are beginning to be characterized. Homeostatic mechanisms have evolved to respond to high and low zinc conditions. The pathway for high zinc homeostasis has been recently elucidated based on the discovery of the master regulator of high zinc homeostasis, HIZR-1. A parallel pathway for low zinc homeostasis is beginning to emerge based on the discovery of the Low Zinc Activation promoter element. Zinc has been established to play a role in two cell fate determination events, and accumulating evidence suggests zinc may function as a second messenger signaling molecule during vulval cell development and sperm activation

Zinc homeostasis and signaling in the roundworm C. elegans

C. elegans is a powerful model for studies of zinc biology. Here we review recent discoveries and emphasize the advantages of this model organism. Methods for manipulating and measuring zinc levels have been developed in or adapted to the worm. The C. elegans genome encodes highly conserved zinc transporters, and their expression and function are beginning to be characterized. Homeostatic mechanisms have evolved to respond to high and low zinc conditions. The pathway for high zinc homeostasis has been recently elucidated based on the discovery of the master regulator of high zinc homeostasis, HIZR-1. A parallel pathway for low zinc homeostasis is beginning to emerge based on the discovery of the Low Zinc Activation promoter element. Zinc has been established to play a role in two cell fate determination events, and accumulating evidence suggests zinc may function as a second messenger signaling molecule during vulval cell development and sperm activation