100% complete assignment of non-labile 1H, 13C, and 15N signals for calcium-loaded calbindin D9k P43G

Here we present the 100% complete assignment chemical shift of non-labile 1H, 15N and 13C nuclei of Calbindin D9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858–14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464–6465, 1994; Yamazaki et al. in Biochem 32:5656–5669, 1993b)

The Dynamics of Ca2+ Ions within the Solvation Shell of Calbindin D9k

The encounter of a Ca2+ ion with a protein and its subsequent binding to specific binding sites is an intricate process that cannot be fully elucidated from experimental observations. We have applied Molecular Dynamics to study this process with atomistic details, using Calbindin D9k (CaB) as a model protein. The simulations show that in most of the time the Ca2+ ion spends within the Debye radius of CaB, it is being detained at the 1st and 2nd solvation shells. While being detained near the protein, the diffusion coefficient of the ion is significantly reduced. However, due to the relatively long period of detainment, the ion can scan an appreciable surface of the protein. The enhanced propagation of the ion on the surface has a functional role: significantly increasing the ability of the ion to scan the protein's surface before being dispersed to the bulk. The contribution of this mechanism to Ca2+ binding becomes significant at low ion concentrations, where the intervals between successive encounters with the protein are getting longer. The efficiency of the surface diffusion is affected by the distribution of charges on the protein's surface. Comparison of the Ca2+ binding dynamics in CaB and its E60D mutant reveals that in the wild type (WT) protein the carboxylate of E60 function as a preferred landing-site for the Ca2+ arriving from the bulk, followed by delivering it to the final binding site. Replacement of the glutamate by aspartate significantly reduced the ability to transfer Ca2+ ions from D60 to the final binding site, explaining the observed decrement in the affinity of the mutated protein to Ca2+

Roadmap on dynamics of molecules and clusters in the gas phase

This roadmap article highlights recent advances, challenges and future prospects in studies of the dynamics of molecules and clusters in the gas phase. It comprises nineteen contributions by scientists with leading expertise in complementary experimental and theoretical techniques to probe the dynamics on timescales spanning twenty order of magnitudes, from attoseconds to minutes and beyond, and for systems ranging in complexity from the smallest (diatomic) molecules to clusters and nanoparticles. Combining some of these techniques opens up new avenues to unravel hitherto unexplored reaction pathways and mechanisms, and to establish their significance in, e.g. radiotherapy and radiation damage on the nanoscale, astrophysics, astrochemistry and atmospheric science

Technology entrepreneurship and creativity as development mechanisms of high-tech organizations

W artykule autorzy opisują kategorię przedsiębiorczości oraz kategorię twórczości technologicznej jako dwa istotne mechanizmy rozwojowe przedsiębiorstw wysokich technologii. Obydwa zagadnienia mieszczą się w obszarze teorii przedsiębiorczości oraz innowacyjności; ze względu na ich specyfikę zaprezentowano je w świetle podejścia konfiguracyjnego. Zgodnie z tym podejściem przywództwo, strategia, struktura oraz otoczenie to cztery podstawowe obszary składające się na wiązki przedsiębiorczego rozwoju organizacji. Główną tezą pracy jest to, że organizacje wysokich technologii wytwarzają różnorakie konfiguracje przedsiębiorczego rozwoju, które są złożone z mechanizmów przedsiębiorczości oraz twórczości technologicznej.The categories of technology entrepreneurship and technology creativity as development mechanisms of high-tech organizations are presented in the paper. These phenomena are presented in the light of management science entrepreneurship theory, and in the context of configuration approach to organization. Leadership, strategy, structure and environment are the four basic domains which consitute the mechanism of entrepreneurial organizational development. The main thesis of the article is that high-tech organizations create various configurations of entrepreneurial development that are consisted of technology entrepreneurship and technology creativity mechanisms

Significance of technological entrepreneurship and creativity in metallurgical enterprises

Tis article presents the categories of technological entrepreneurship and creativity that constitute a new perspective on developmental mechanisms of contemporary enterprises. These mechanisms are of particular significance in relation to industrial enterprises, including metallurgic ones. Forthe purposes of thispublicationthe significance of technological creativity and entrepreneurship is described with the examples of metallurgical enterprises

Forecast of the labor market for professional drivers in Poland by the 2020

W artykule zaprezentowano rozważania dotyczące problematyki liczby kierowców, która przypuszczalnie będzie niezbędna do funkcjonowania przewozów samochodowych w Polsce.A brief assessment of the current situation and prospects of development of the Polish road transport. Estimation of the number of trucks over 3.5 tones gross vehicle weight and buses in Poland by the end of 2010 and the forecast in perspective by the 2020. Estimation of the current number of active professional drivers in Poland. Forecast of professional drivers by the 2020 and the number of drivers who should have, every year, periodic training. Discussion about possible reasons for the lack of an adequate supply of professional drivers in the labor market

The sources of strategic threats that are specific to high-tech innovative companies

W artykule zawarto wyniki badań sondażowych, dotyczących zagrożeń dla przedsiębiorstw innowacyjnych wysokich technologii oraz zagrożeń jako sytuacji kryzysowych oraz sformułowano wyjściowe, podstawowe rekomendacje dla doskonalenia zarządzania strategicznego w tych organizacjach. Wnioski i postulaty sformułowano na podstawie badań sondażowych, prowadzonych w trakcie realizacji projektu - grantu badawczego.The paper contains the results of surveys regarding the risks for innovative companies, high-tech and threats as emergencies and formulated output, basic recommendations for improving the strategic management of these organizations. Conclusions and proposals were formulated on the basis of surveys conducted during the project - a research grant