Elements of oxidation/reduction balance in experimental hypothyroidism

Background: The aim of this study was to investigate the effect of the decreased level of thyroid hormones on selected parameters of the oxidation/reduction balance by assessing the activity of antioxidant enzymes: superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px); the level of antioxidant vitamins (A, C, and E); and the concentration of compounds reacting with thiobarbituric acid (TBARS). Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed: — in blood serum, the thyroid hormones T3, T4 and TSH; — vitamin A, C and E blood serum concentrations; — in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px. Results: A 21-day exposure of rabbits to thiamazole (2 mg/kg b.w./24 h) resulted in a statistically significant decrease of TBARS, a decrease of SOD and GPH-Px activity and in a statistically insignificant decrease in the level of vitamins A, C and E. Conclusions: Hypothyroidism decreases the level of erythrocytes oxidation/reduction balance by diminishing oxidative lipids damage and by decreasing the activity of antioxidative enzymes, but not by changes in the level of antioxidant vitamins. (Pol J Endocrinol 2011; 62 (3): 220–223)Wstęp: Celem badania była ocena wpływu zmniejszenia stężenia hormonów tarczycy na wybrane parametry równowagi oksydacyjno-redukcyjnej przez badanie aktywności enzymów antyoksydacyjnych ( SOD, GSH-Px), stężenia witamin antyoksydacyjnych (A, C, E) oraz związków reagujących z kwasem tiobarbiturowym (TBARS). Materiał i metody: Badania przeprowadzono na 40 królikach rasy olbrzym beligijski, obojga płci. Niedoczynność tarczycy wywołano dożołądkowym podawaniem thiamazolu. Przed podaniem thiamazolu pobrano krew żylną (grupa kontrolna), a następnie przez 21 dni zwierzęta otrzymywały dożołądkowo thiamazol w dawce 2 mg/kg mc. Po tym czasie ponownie pobierano krew (grupa badana) i oznaczano: — stężenie hormonów tarczycy T3, T4 i TSH; — stężenie witamin A, C i E w surowicy; — stężenie związków reagujących z kwasem tiobarbiturowym (TBARS) i aktywność dysmutazy ponadtlenkowej (SOD) i peroksydazy glutationowej (GPH-Px) w erytrocytach. Wyniki: Po 21 dniach dożołądkowego podawania królikom thiamazolu (2 mg/kg mc./24 h) stwierdzono statystycznie znamienne zmniejszenie stężenia TBARS, obniżenie aktywności SOD i GPH-Px oraz statystycznie nieznamienne obniżenie stężenia witaminy A, C i E. Wnioski: Niedoczynność tarczycy obniża poziom równowagi oksydacyjno-redukcyjnej erytrocytów poprzez zmniejszenie oksydacyjnych uszkodzeń tłuszczów oraz zmniejszenie aktywności enzymów antyoksydacyjnych, a nie poprzez zmiany w stężeniu witamin antyoksydacyjnych. (Endokrynol Pol 2011; 62 (3): 220–223

Effect of anthocyanins on selected biochemical parameters in rats exposed to cadmium.

Cadmium is a dangerous occupational and environmental toxin. It accumulates in the human organism mainly in liver and kidneys. Cadmium half-life is about 10 years, so the symptoms of cadmium intoxication may occur several years after the exposure. Until now in treating intoxication with this metal chelating compounds have been used, burdened with numerous undesirable symptoms. In our investigations anthocyanins from Aronia melanocarpa were used to reduce the harmful results caused by cadmium. Administering anthocyanins with cadmium chloride resulted in a statistically significant decrease of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, concentration of bilirubin and urea in blood serum and decreased cadmium cumulation in liver and kidneys in relation to animals receiving cadmium chloride only

Prace oryginalne/original PaPers Elements of oxidation/reduction balance in experimental hypothyroidism Elementy bariery oksydacyjno-redukcyjnej w doświadczalnej hypotyreozie

Abstract Background: The aim of this study was to investigate the effect of the decreased level of thyroid hormones on selected parameters of the oxidation/reduction balance by assessing the activity of antioxidant enzymes: superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px); the level of antioxidant vitamins (A, C, and E); and the concentration of compounds reacting with thiobarbituric acid (TBARS). Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed: -in blood serum, the thyroid hormones T3, T4 and TSH; -vitamin A, C and E blood serum concentrations; -in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px