Survey on alimentary tract parasites in cultured fish in Khouzestan province

In this study which was carried out in khoozestan province, 1100 pieces of common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix), Grass carp (Ctenopharyngodon idella) and Big head (Arysthychthys nobilis) from different parts of the province were examined. From each part, 10% of the fish farms were sampled according to the fish density at each farm. parasitic infection of gastrointestinal tract were studied on 600 pieces of silver carp (60% ) , 244 pieces of common carp (25%) , 51 pieces of Big head (5%) . only Grass carp was infested to protozoan and metazoan parasites . thirty percent of examined Grass carp of 2-730 gr weight were infested to Hexamita but infestation was more severe in Grass carp with 20-40 gr weight . macroscopic manifestation included : dysphagia , weakness , circulating movements, paling of gills , stomach inflammation , intestinal inflammation , ascitis and yellowish gut contents . in severe and intestinal necrosis along with hyperemia was apparent . the cestoda , bothriocephalus opsariichthydis ( Rudolphi , 1808 ) with an infestation rate of 35 % were only observed in Grass carp in 2-40 gr . Grass carp that showed severe infestation, intestinal blockade was observed with more than 100 parasites. The length of adult cestoda was 450-530 mm and its width was 5mm. in severe infestations, macroscopic manifestations included: ascitis, abdominal inflammation, swimming on water surface and emaciation. Histologic studies showed necrosis of intestinal wall, hemorrhage and localized necrosis. In fishes with severe infestations, inflammation was observed. Infestation with the nematode, capilaria was observed in 10% of 20-750 gr Grass carp. No macroscopic symptoms were identified in the samples, but microscopic examination showed acute necrosis along with hyperamia and submocusal hemorrhage. Lymphocytes were also observed. Adult nematode had a width of 0.075 and a length of 7.5-8.5 mm

Survey on health status in aquaculture sturgeons centers (Mazandaran, Guilan And Golestan Provinces)

Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p>0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05)