Continuous downstream process of monoclonal antibody developed based on the process analysis/understanding and its validation

As downstream process (DSP) of monoclonal antibody (mAb) includes several batch chromatography steps, and other operations such as virus inactivation (VI), it is important to consider how each operation is carried out in order to make an efficient continuous or pseudo-continuous DSP. Our consortium* developed a continuous DSP (CDSP) of mAb under the AMED** project “Development of platform technologies for the continuous manufacturing of biopharmaceuticals”, and successfully carried out several runs at our GMP facility and laboratory. In this paper, we will present how our CDSP was developed based on the process analysis and understanding, and the purification results along with several important issues to be addressed in the future [1]. For the first “capture” step, a 2-column “periodic counter-current chromatography (PCCC)” with protein A chromatography columns was chosen. The efficiency of PCCC is strongly dependent on the operating conditions, which should be determined properly [2, 3]. Please click Additional Files below to see the full abstract

Development of Health Literacy from Activities of Culture, Leisure, and Sports

Living in a healthy and independent manner would be beneficial for people. Health literacy can be obtained through activities of culture, leisure, and sports. Authors have been involved in sports medicine, music, and management of International Masters Athletics Federation（IMAF）. Health literacy would be a dynamic multi-dimensional perspective that combines various kinds of health-related matter, information, and activity. It can be developed through the activities of sports and music. These opportunities are divided into scheduled care healthcare programs and unexpected human interaction with various participants together. The latter would be a synergistic effect in multi-dimensional aspects

Perspectives of Music and Sports for Satisfactory Anti-Aging Daily Life

Aging societies have been observed in Japan and other developed countries worldwide. In the light of anti-aging medicine, authors have continued several approaches such as Integrative Medicine (IM), music therapy, art therapy, frailty, rehabilitation, sports medicine and masters’ athletics. For successful aging, three important factors are necessary, which are i) no illness or disability, to maintain physical/cognitive function, to continue social participation and contribution. Music has large power similar to language and communication and clinical effects physically, psychologically and socially. Various plays of the activities for elderly such as music and sports can bring processes towards achieving successful aging

Comparison of non-invasive, scalp-recorded auditory steady-state responses in humans, rhesus monkeys, and common marmosets

Auditory steady-state responses (ASSRs) are basic neural responses used to probe the ability of auditory circuits to produce synchronous activity to repetitive external stimulation. Reduced ASSR has been observed in patients with schizophrenia, especially at 40 Hz. Although ASSR is a translatable biomarker with a potential both in animal models and patients with schizophrenia, little is known about the features of ASSR in monkeys. Herein, we recorded the ASSR from humans, rhesus monkeys, and marmosets using the same method to directly compare the characteristics of ASSRs among the species. We used auditory trains on a wide range of frequencies to investigate the suitable frequency for ASSRs induction, because monkeys usually use stimulus frequency ranges different from humans for vocalization. We found that monkeys and marmosets also show auditory event-related potentials and phase-locking activity in gamma-frequency trains, although the optimal frequency with the best synchronization differed among these species. These results suggest that the ASSR could be a useful translational, cross-species biomarker to examine the generation of gamma-band synchronization in nonhuman primate models of schizophrenia

On-demand transfer of trapped photons on a chip

チップ上の微小点に保存した光の瞬時転送. 京都大学プレスリリース. 2016-05-26.Photonic crystal nanocavities, which have modal volumes of the order of a cubic wavelength in the material, are of great interest as flexible platforms for manipulating photons. Recent developments in ultra-high quality factor nanocavities with long photon lifetimes have encouraged us to develop an ultra-compact and flexible photon manipulation technology where photons are trapped in networks of such nanocavities. The most fundamental requirement is the on-demand transfer of photons to and from the trapped states of arbitrary nanocavities. We experimentally demonstrate photon transfer between two nearly resonant nanocavities at arbitrary positions on a chip, triggered by the irradiation of a third nonresonant nanocavity using an optical control pulse. We obtain a high transfer efficiency of ～90% with a photon lifetime of ～200 ps

Role of anion size, magnetic moment, and disorder on the properties of the organic conductor kappa-(BETS)_2Ga_{1-x}Fe_{x}Cl_{4-y}_Br_{y}

Shubnikov-de Haas and angular dependent magnetoresistance oscillations have been used to explore the role of anion size, magnetic moment, and disorder in the organic conductors kappa-(BETS)_2GaBr_{4} and kappa-(BETS)_2FeCl_{2}_Br_{2} in the isomorphic class kappa-(BETS)_2Ga_{1-x}Fe_{x}Cl_{4-y}_Br_{y}. The results, combined with previous work, show correlations between the anion composition (Ga_{1-x}Fe_{x}Cl_{4-y}_Br_{y}) and the superconducting transition temperature, effective mass, Fermi surface topology, and the mean free path.Comment: 5 pages, 6 figure