Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology

Abstract Methodological advances in on-chip technology enable high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process. This technology is, however, limited to batch manipulation of the droplet-array. Here, we propose a method for selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic–hydrophobic patterns for droplet formation 4 μl in volume were developed. We demonstrate the selective control of the droplet height by EWOD for an applied voltage up to 160 V and selective control of the contact and transport of substances. Presented results will provide useful method for advanced drug screening, including cell-based screening

Classroom practice for understanding pointers using learning support system for visualizing memory image and target domain world

Abstract Pointers are difficult learning targets for novice learners of C programming. For such difficult targets, introducing a system visualizing program behaviors is generally expected to support learners to understand the targets. However, visualization in existing systems often conceals the concrete value of variables such as pointers; the way in which each visualized object is located on the memory is not made explicit. In order to address this issue, we focused on a program visualization system called TEDViT. It visualizes simultaneously and synchronously the memory image that is the field that presents the concrete value of variables and the target domain world that is the field that presents logically the data structures processed by the program. We consider that observing and comparing program code, memory image, and target domain world with TEDViT could work for understanding pointers. TEDViT visualizes the status of the target domain world according to the visualization policy defined by the teacher in order to allow teachers to set their instruction content based on the growing variety of learner background knowledge. We also consider that this feature could support teachers’ instructions and class managements appropriately, and improving teachers’ performance by TEDViT’s support would bring improvement of learners’ understanding. We conducted classroom practice for understanding pointers in connection with a memory model, thus introducing TEDViT to a real class. Analysis of answered scores in a questionnaire conducted after the practice suggests that our practice using TEDViT provided useful supports for participants to understand pointers. It also suggests our practice had a certain effect to reduce uneven levels of understanding among participants. Based on these results, we describe that classroom practices in our framework could support learners to understand pointers and support teachers to manage the class

Risk factors associated with cytomegalovirus reactivation in patients receiving immunosuppressive therapy for rheumatic diseases: a retrospective study

Abstract Immunosuppressive treatment is a common cause of cytomegalovirus (CMV) reactivation. However, there is no consensus regarding the risk factors for CMV reactivation in rheumatic diseases. Therefore, this study aimed to elucidate the risk factors associated with CMV reactivation. We retrospectively collected the data of 472 patients with rheumatic diseases whose CMV pp65 antigen (C7-HRP) titer was measured. We divided the patients into those with and those without C7-HRP. We retrospectively collected data on age, sex, primary condition and organ involvement, and blood test results. We also investigated the use of immunosuppressants and the maximum and cumulative doses of prednisolone (PSL). We performed univariate and multivariate analyses to identify risk factors for CMV reactivation. Multivariate analysis showed that higher age (71.2 vs. 64.4 years, p = 0.0022), hypoalbuminemia (2.9 vs. 3.4 g/dL, p = 0.0104), higher creatinine level (1.2 vs. 0.9 mg/dL, p = 0.0026), cyclosporine use (8.2 vs. 3.6%, p = 0.0101), and higher maximum (552.4 vs. 243.3 mg, p < 0.0001) and cumulative (2785.9 vs. 1330.5 mg, p < 0.0001) doses of PSL were associated with CMV reactivation. Older age, hypoalbuminemia, higher creatinine level, cyclosporine use, and higher maximum and cumulative doses of PSL were significant risk factors for CMV reactivation in rheumatic diseases