Assessment of carcinogenic risk levels to the health of population of some cities of Bashkortostan

The results of individual and population cancer risk assessment to the health of population of some cities in the Republic of Bashkortostan under the influence of air chemical pollutants and drinking water are given. Research has shown that in the examined cities, the risk to public health from air chemical contamination is higher than from drinking water contamination.Приведены результаты оценки индивидуального и популяционного канцерогенного риска для здоровья населения некоторых городов Республики Башкортостан от воздействия химических веществ, загрязняющих атмосферный воздух и питьевую воду. Исследования показали, что в настоящее время в изученных городах риск для здоровья населения от химического загрязнения атмосферного воздуха выше, чем от загрязнения питьевой воды

Genotoxicity of hyroquninon in A549 cells

Hydroquinone (HQ) is found in natural and anthropogenic sources including food, cosmetics, cigarette smoke, and industrial products. In addition to ingestion and dermal absorption, human exposure to HQ may also occur by inhaling cigarette smoke or polluted air. The adverse effects of HQ on respiratory systems have been studied, but genotoxicity HQ on human lung cells is unclear. The aim of this study was to investigate the cytotoxicity and genotoxicity of HQ in human lung alveolar epithelial cells (A549). We found that HQ induced a dose response in cell growth inhibition and DNA damage which was associated with an increase in oxidative stress. Cytotoxicity results demonstrated that HQ was most toxic after 24 h (LC50 = 33 μM) and less toxic after 1 h exposure (LC50 = 59 μM). Genotoxicity of HQ was measured using the Comet assay, H2AX phosphorylation, and chromosome aberration formation. Results from the comet assay revealed that DNA damage was highest during the earlier hours of exposure (1 and 6 h) and thereafter was reduced. A similar pattern was observed for H2AX phosphorylation suggesting that damage DNA may be repaired in later exposure hours. An increase in chromosomal aberration corresponded with maximal DNA damage which further confirmed the genotoxic effects of HQ. To investigate whether oxidative stress was involved in the cytotoxic and genotoxic effects of HQ, cellular glutathione and 8-Oxo-deoguanisone (8-Oxo-dG) formation were measured. A decrease in the reduced glutathione (GSH) and an increase oxidized glutathione (GSSG) was observed during the early hours of exposure which corresponded with elevated 8-Oxo-dG adducts. Together these results demonstrate that HQ exerts its cytotoxic and genotoxic effects in A549 lung cells, probably through DNA damage via oxidative stress