Causative Role of Sexually Transmitted Infections in the Development of Chronic Cystitis Complicated with Leukoplakia of the Bladder

The objective of this study was the investigation of the influence of chlamydial, mycoplasmal and trichomonas infection on the development of urinary bladder leukoplakia. The article presents the results of the examination of women with chronic cystitis complicated with leukoplakia of the bladder, and associated with concomitant sexually transmitted infections, including the results of culture analysis of the cervical canal content and urinary bladder biopsy samples, as well as molecular-biological analyses confirming the presence of sexually transmitted infections, pathomorphological characteristics of tissue samples from leukoplakia foci typical for different types of infectious agents. In this study, 60 women with chronic cystitis, complicated with leukoplakia of the bladder and associated with concomitant sexually transmitted infections were examined. Using PCR diagnostics, Mycoplasma hominis and Chlamydia albicans were found to be the most frequently occurring agents, followed by Ureaplasma urealyticum, Chlamydia trachomatis and Trichomonas vaginalis. The results of culture analyses demonstrated that M. hominis and U. urealyticum were prevalent in patients with chronic urinary tract inflammatory processes, followed by Tr. vaginalis. Candida fungi show practically the same frequency of occurrence. The pathomorphological examination of the foci of leukoplakia in the urinary bladder (in 30 subjects) demonstrated metaplasia of the transitional epithelium to the stratified pavement squamous epithelium with inflammatory cellular infiltration of the lamina propria in all types of infections. The intensity of the urothelial transformation and stromal inflammatory processes were determined by the type of predominant infection. Pathomorphological characteristics of the foci of leukoplakia correlate with the etiology of chronic inflammation and are relevant for etiological diagnosis and treatment

Causative Role of Ureaplasma Urealyticum and other Sexually Transmitted Infections in the Urethral Meatus Polyp Development in Women

The objective of this study was the investigation of the influence of ureaplasmal infection on the development of urethral meatus polyps in women. The article presents the results of the examination of women with chronic cystitis and urethritis over a 0.5- to 5-year duration, complicated by the presence of urethral meatus polyps and associated with concomitant Ureaplasma urealyticum and other sexually transmitted infections (STI). This was based on the culture analysis of the cervical and urethral content, and PCR-diagnostics of STI, as well as a complex pathomorphologic study of the resected polyps, including electron microscopy. In this study, 98 women between 45 and 60 years (52.5±4.9 years) were examined, who had undergone radiowave resection of the polyps: 52 women were infected by STI, including Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Chlamydia trachomatis and Trichomonas vaginalis, while the remaining 46 women had been diagnosed as not having STI. According to the culture results in the women with STI, U. urealyticum was identified as a monoinfection in 69% of cases, while in the remaining 31% of cases it was evident in the form of mixed infections, mainly in association with Mycoplasma hominis (17.5%) and Trichomonas vaginalis (13.5%). Pathomorphological examination of the urethral meatus polyps of the women with U. urealyticum and other STI demonstrated the proliferative character of the remodeling of the surface epithelium with hyperplasia, acanthosis, and keratinization of the stratified squamous epithelium and synchronous changes in the underlying connective tissue - impaired microcirculation and the diffuse inflammatory cell infiltrates with transepithelial leukopedesis. Using electron microscopy in the fibroblasts and plasma cells of the resected polyps the markers of U. urealyticum were detected in patients with negative results of the bacteriological diagnostic methods

Strategies for the Mediatization of Legal Concepts in Russian Mass Media of XXI Century (Concept LEGITIMACY)

The role of mass media in the processes of interpretation and assessment of various fragments of reality is discussed in the article. The problem of the entry of legal concepts into the media discourse is raised and the task is set to study the changes occurring in this process, which ultimately lead to the formation of new conceptual units — media concepts. The designated task is solved in the process of analyzing one of the key legal concepts LEGITIMACY. It has been demonstrated that the Russian mass media significantly modify each of the three components of the legal concept, that is, the media virus is “implanted” into the conceptual, figurative and value layers. It allows us to speak of three strategies of mediatization: definitional, metaphorical and evaluative. It is shown that the definitional strategy contributes to the emergence of new features in the conceptual layer of the LEGITIMACY concept (‘trust’, ‘approval’, ‘support’, ‘respect’, ‘fairness’, ‘the phenomenon of consciousness’ and ‘subjective feeling’). It is noted that the metaphorical strategy additionally forms such linguo-culturally specific features as ‘defectiveness’ and ‘object of political manipulation’, the evaluative strategy gives the positively perceived concept of LEGITIMACY a negative connotation (conceptual signs of ‘doubtfulness’ and ‘imaginary’ develop), which is an alarming marker reflecting the current situation in Russian society. It is pointed out that the recorded changes indicate the importance of the discursive factor in the study of concepts

Microwave-Assisted Hydrothermal Synthesis and Annealing of DyF 3

The series of DyF3 nanosized samples was synthesized by the colloidal chemistry method. The microwave-assisted hydrothermal treatment was used for the first time for the modification of DyF3 nanoparticles. Transmission electron microscopy images show that the DyF3 nanoparticles have average particle size of about 16–18 nm and the size distribution becomes narrower during the microwave irradiation. The X-ray diffraction analysis shows the narrowing of the diffraction peaks versus microwave treatment time. The experimental data demonstrates restructuring of the nanoparticles and their crystal structure becomes closer to the ideal DyF3 regular structure during the microwave irradiation of colloidal solution. The defect-annealing model of the microwave-assisted hydrothermal modification process is suggested

MAINTENANCE OF СА2+-ATP-ASE AMOUNT IN SARCOPLASMATIC RETICULUM CARDIOMYOCYTES IN ISCHEMIC MYOCARDIUM DURING SHORT DURATION OF DIABETES MELLITUS COURSE Kondratyeva D. S., Afansyev S. A., Kanev A. F., Kozlov B. N.

Aim. To study the level of Са2+-ATP-ase of sarcoplasmatic reticulum (SR) in human myocardium in ischemic heart disease (CHD) and in CHD with diabetes mellitus 2nd type (DM), and also in animals with postinfarction cardiosclerosis with DM.Material and methods. The study performed on biopsy material of the hearts from patients and rats with chronic ishemic heart disease wih DM. A piece of left atrial appendage (biopsy material) was cut away during the period of the artificial circulation device setup. Postinfarction cardiosclerosis (PICS) had being formed during 6 weeks after coronary artery occlusion. DM developed during 6 weeks after intraperitoneal injection of streptosotocine (60 mg/kg). The level of Са2+-ATP-ase was measured by immuneblotting.Results. It was shown that in both groups there were patients, whose myocardium contained high level of Са2+-ATP-ase and patients with low levels of this protein. At the same time myocardium of patients with CHD having high level of Са2+-ATP-ase, contained by 26,6%±0,53% (p<0,05) more of the protein studied, than myocardium of CHD patients with DM. Comparison of low Са2+-ATP-ase level patients did not show statistically significant difference between groups. Measurement of Са2+-ATPase in animals’ myocardium showed that ischemic and diabetic remodeling of myocardium leads to the decrease of Са2+-ATP-ase CP to 38,6±0,92% and 49±2,71%, resp. Concomitant development of these pathologies characterizes by the lowest amount of the protein studied and is 52,3±2,6% from controls.Conclusion. Myocardium of CHD patients, and rats with PICS shows higher level of Са2+-ATP-ase with DM with short disease duration

EFFICIENCY OF «AB INTERNO» TRABECULECTOMY USING THE «TRABECTOME» OPERATING SYSTEM IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA AND CATARACT

Purpose.  To study dynamics of intraocular pressure in open-angle glaucoma and cataract patients after «ab interno» trabeculectomy with the use of the «Trabectome» system. Material and methods. There were operated 52 patients (63 eyes) with open-angle glaucoma. The IOP level was determined using the Maklakov tonometer before operation and 1 week, 2 weeks, 3-5, 6-8, 9-12, 13-26, 27 and more weeks after operation. Results.  In patients with cataract and glaucoma the mean IOP before operation was 26.1±3.23mmHg and after operation – 20.5±3.23mmHg in the mean follow-up 173± 170.2 days. Conclusions. «Ab interno» trabeculectomy with the use of the «Trabectome» operating system allowed to decrease the IOP by 21.5% in glaucoma patients. It is possible because of microinvasive removal of the trabecular tissue as the main reason for a resistance to the aqueous humor outflow in open-angle glaucoma patients

Diabetes mellitus type 2 and acute myocardial infarction: prognostic options for interaction in patients of different age groups

Background. Problems surrounding comorbidities of type 2 diabetes mellitus and coronary heart disease are some of the most important in medical science and practice, given their mutually negative impact on patients prognoses and quality of life. Aims. To study the impact of type 2 diabetes on the long-term prognoses of patients of different age categories, status-post acute myocardial infarction. (Data obtained from the Register of Acute Myocardial Infarction.) Materials and methods. The main data source was the Register of Acute Myocardial Infarction, maintained in Tomsk for more than 30 years. The study included 862 patients with acute myocardial infarction. The patients were monitored for 5 years. The primary endpoint was death from any cause during the observation period. Results. We separated the study cohort into 2 groups depending on patients ages: Group 1 (n = 358) included patients older than working age, Group 2 (n = 504) consisted of younger, employable patients. The combination of ischaemic heart disease and type 2 diabetes mellitus were diagnosed in 208 patients. The combination of ischaemic heart disease and type 2 diabetes was the cause of adverse prognosis among elderly patients and led to increased mortality rate during the 5-year post-infarction period (p = 0.0003). However, among younger, working patients who suffered myocardial infarction, the presence of type 2 diabetes did not have an independent negative effect on long-term disease prognosis. While in employable patients, a long history of diabetes mellitus significantly aggravated the course of the post-infarction period (p = 0.004). Conclusions. These data suggest an ambiguous prognostic effect of type 2 diabetes mellitus among working age and elderly patients status post myocardial infarction, in agreement with experimental studies conducted on laboratory animals. Further comprehensive analyses of the clinical and experimental data are needed to optimise therapies for patients who suffer from type 2 diabetes and comorbid ischaemic heart disease

New Antioxidant Genes from an Anhydrobiotic Insect: Unique Structural Features in Functional Motifs of Thioredoxins

© 2016, Springer Science+Business Media New York.Polipedilum vanderplanki is the most complex known organism able to survive body desiccation via entering a state of suspended metabolism called anhydrobiosis. This unique ability is based on the specific molecular machinery involving a synthesis of non-reducing sugar trehalose and a variety of protective proteins. Genes encoding these protective proteins are extensively duplicated in the P. vanderplanki genome and become hugely upregulated in response to desiccation. Some of these highly expressed genes encode substitutions of amino acids crucial for the function of corresponding proteins. An intriguing group of protective proteins in P. vanderplanki are thioredoxins (TRX). These antioxidant proteins are important for P. vanderplanki anhydrobiosis since desiccation is tightly related to the elevated production of free radicals and oxidative damage. The TRX set is unprecedentedly expanded in the P. vanderplanki genome up to 25 TRX genes. Genomes of congeneric midge Polipedilum nubifer, Apis mellifera, Drosophila melanogaster, and Anopheles gambiae encode only 3–7 TRX genes. Moreover, all three P. vanderplanki thioredoxin genes most expressed at 24 h of P. vanderplanki larva desiccation encode for proteins missing the typical CxxC motif