Moderate intensity exercise in hypoxia increases IGF-1 bioavailability and serum irisin in individuals with type 1 diabetes

Aim: This study aimed to determine the effect of moderate intensity continuous exercise (Ex) and hypoxia (Hyp) on serum brain-derived neurotrophic factor (BDNF), insulin-like growth factor-1 (IGF-1) and its binding protein-3 (IGFBP-3), irisin and cytokines levels in patients with type 1 diabetes (T1D). Methods:: A total of 14 individuals with T1D (age: 28.7 ± 7.3 years) and 14 healthy adults (age: 27.1 ± 3.9 years) performed 40-min continuous Ex at moderate intensity (50% lactate threshold) on a cycle ergometer in normoxia (Nor) and Hyp (FiO2 = 15.1%) Biochemical factors, glucose concentrations and physiological variables were measured at rest, immediately and up to 24 h after both Ex protocols. Results:: Patients with T1D had significantly lower pre-Ex serum concentrations of BDNF (p < 0.05, p < 0.01), and total IGF-1 (p < 0.001, p < 0.05) and significantly higher irisin levels (p < 0.05, p < 0.01) in Nor and Hyp, compared with healthy subjects. Ex significantly increased in T1D group serum BDNF (in Nor only p < 0.05) and total IGF-1 levels in Nor and Hyp (p < 0.001 and p < 0.01, respectively). Immediately after Ex in Hyp, freeIGF-1 (p < 0.05) and irisin levels (p < 0.001) were significantly higher compared with the levels induced by Ex alone. Free IGF-1 and irisin serum levels remained elevated in 24 h post-Ex in Hyp. In T1D, significant blood glucose (BG) decrease was observed immediately after Ex in Hyp (p < 0.001) and in 24 h recovery (p < 0.001) compared with pre-Ex level. Conclusion:: The study results suggest that moderate intensity continuous Ex has beneficial effect on BDNF and IGF-1 levels. Ex in hypoxic conditions may be more effective in increasing availability of IGF-1. The alterations in the post-Ex irisin levels and IGF-1 system may be contributing to more effective glycaemia control in patients with T1D

Unique Thermal Properties of Clothing Materials.

Cloth wearing seems so natural that everyone is self-deemed knowledgeable and has some expert opinions about it. However, to clearly explain the physics involved, and hence to make predictions for clothing design or selection, it turns out to be quite challenging even for experts. Cloth is a multiphased, porous, and anisotropic material system and usually in multilayers. The human body acts as an internal heat source in a clothing situation, thus forming a temperature gradient between body and ambient. But unlike ordinary engineering heat transfer problems, the sign of this gradient often changes as the ambient temperature varies. The human body also perspires and the sweat evaporates, an effective body cooling process via phase change. To bring all the variables into analysis quickly escalates into a formidable task. This work attempts to unravel the problem from a physics perspective, focusing on a few rarely noticed yet critically important mechanisms involved so as to offer a clearer and more accurate depiction of the principles in clothing thermal comfort

Characteristics of the competitive loads of Polish National Amputee Football Team. Pilot studies

Background: Information about competitive loads of amputee soccer players are very limited. This element should be one of the base to prepare appropriate training program according to individual needs of players, as well aims of season. The main aim of this pilot study was to describe the characteristics of selected elements of the internal competitive load of football players after unilateral amputations. Methods: The subject involved a group of 15 Polish National Amp Football (PNAF) players after unilateral amputations of t he upper or lower limbs. The age, height and weight were 27.1±8.4 years, 176.2±7.3 cm, 72.0±14.2 kg, respectively. The information were recorded during match between PNAF Team and representative of Polish Clubs, using Polar Team 2Pro System. The data base was analyzed using standard tools and differences among variables was checked by Wilcoxon test. Results: During whole games value of HRmean was 132.6±10.56 bpm with a range from 87.3±10.6 bpm to 182.3±10.69 bpm and energy expenditure was 612.7±164.86 kcal. General, Values of observed variables were higher in the 2nd part of the match but differences among them was not significance. Conclusion: Characterizing the competitive effort will allow to diagnose and determine the nature of the work to use it as specific requirements of the discipline. It can be expected that these information will contribute to the improvement the training process for AMP Footballers and optimize training of the disabled people

Molecular Cell Short Article trans-Splicing to Spliceosomal U2 snRNA Suggests Disruption of Branch Site-U2 Pairing during Pre-mRNA Splicing

SUMMARY Pairing between U2 snRNA and the branch site of spliceosomal introns is essential for spliceosome assembly and is thought to be required for the first catalytic step of splicing. We have identified an RNA comprising the 5 0 end of U2 snRNA and the 3 0 exon of the ACT1-CUP1 reporter gene, resulting from a trans-splicing reaction in which a 5 0 splice site-like sequence in the universally conserved branch site-binding region of U2 is used in trans as a 5 0 splice site for both steps of splicing in vivo. Formation of this product occurs in functional spliceosomes assembled on reporter genes whose 5 0 splice sites are predicted to bind poorly at the spliceosome catalytic center. Multiple spatially disparate splice sites in U2 can be used, calling into question both the fate of its pairing to the branch site and the details of its role in splicing catalysis

Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs

Copyright @ 2011 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Spliceosomes remove introns from primary gene transcripts. They assemble de novo on each intron through a series of steps that involve the incorporation of five snRNP particles and multiple non-snRNP proteins. In mammals, all the intermediate complexes have been characterized on one transcript (MINX), with the exception of the very first, complex E. We have purified this complex by two independent procedures using antibodies to either U1-A or PRPF40A proteins, which are known to associate at an early stage of assembly. We demonstrate that the purified complexes are functional in splicing using commitment assays. These complexes contain components expected to be in the E complex and a number of previously unrecognized factors, including survival of motor neurons (SMN) and proteins of the SMN-associated complex. Depletion of the SMN complex proteins from nuclear extracts inhibits formation of the E complex and causes non-productive complexes to accumulate. This suggests that the SMN complex stabilizes the association of U1 and U2 snRNPs with pre-mRNA. In addition, the antibody to PRPF40A precipitated U2 snRNPs from nuclear extracts, indicating that PRPF40A associates with U2 snRNPs

Heart rate variability (HRV) and muscular system activity (EMG) in cases of crash threat during simulated driving of a passenger car

Objectives: The aim of the study was to verify whether simultaneous responses from the muscular and circulatory system occur in the driver's body under simulated conditions of a crash threat. Materials and Methods: The study was carried out in a passenger car driving simulator. The crash was included in the driving test scenario developed in an urban setting. In the group of 22 young male subjects, two physiological signals - ECG and EMG were continuously recorded. The length of the RR interval in the ECG signal was assessed. A HRV analysis was performed in the time and frequency domains for 1-minute record segments at rest (seated position), during undisturbed driving as well as during and several minutes after the crash. For the left and right side muscles: m. trapezius (TR) and m. flexor digitorum superficialis (FDS), the EMG signal amplitude was determined. The percentage of maximal voluntary contraction (MVC) was compared during driving and during the crash. Results: As for the ECG signal, it was found that in most of the drivers changes occurred in the parameter values reflecting HRV in the time domain. Significant changes were noted in the mean length of RR intervals (mRR). As for the EMG signal, the changes in the amplitude concerned the signal recorded from the FDS muscle. The changes in ECG and EMG were simultaneous in half of the cases. Conclusion: Such parameters as mRR (ECG signal) and FDS-L amplitude (EMG signal) were the responses to accident risk. Under simulated conditions, responses from the circulatory and musculoskeletal systems are not always simultaneous. The results indicate that a more complete driver's response to a crash in road traffic is obtained based on parallel recording of two physiological signals (ECG and EMG)

Alder pollen season in poland in 2018

Alder pollen grains constitute the important allergen sources in this respect in the Northern Hemisphere. The aim of the study was to investigate the concentration of alder (Alnus spp.) in Bialystok, Bydgoszcz, Cracow, Drawsko Pomorskie, Lublin, Olsztyn, Opole, Piotrkow Trybunalski, Sosnowiec, Szczecin, Warsaw, Wroclaw and Zielona Gora in 2018. Measurements were performed by the volumetric method (Burkard and Lanzoni pollen samplers). Seasonal Pollen Index (SPI) was estimated as the annual sum of daily average pollen concentrations. The pollen season of alder in all Polish stations began on the 11th and 13th March and the high concentration persisted until the first days of April. The highest, record airborne concentration of 1068 pollen grains/m3 was noted in Lublin on the 13th March. The peak values of seasonal pollen count occurred between of 11th March and 4th April in all cities. In 2018 pollen concentration of alder was one of the lowest in all analysed cities

Analysis of the plantain pollen season in selected Polish cities in 2018

The paper presents the course of pollen season of plantain in Bialystok, Bydgoszcz, Drawsko Pomorskie, Cracow, Lublin, Olsztyn, Opole, Piotrkow Trybunalski, Sosnowiec, Szczecin, Warsaw, Wroclaw and Zielona Gora in 2018. Measurements were performed by the volumetric method (Burkard and Lanzoni pollen samplers). Pollen season was defined as the period in which 98% of the annual total catch occurred. The plantain pollen season started in the first decade of May and lasted until the end of September. Despite such a long pollen season in Poland, only in some cities there are days with an average concentration exceeding 10 P/m3. Significant differences were observed in annual sum values. The highest values were recorded in Lublin (400 grains) and Zielona Gora (308 grains), and the lowest in Drawsko Pomorskie (160 grains) and Olsztyn (184 grains). The value of annual average in 2018 was usually lower than in the previous years

The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells

GFP-tagged snRNP components reveal the dynamics and rate for spliceosome assembly in vivo

The oak pollen concentration in the air of selected cities in Poland in 2018

This paper contains an analysis of oak pollen seasons in selected cities of Poland in 2018. Sampling sites were located in the following cities: Bialystok, Bydgoszcz, Cracow, Drawsko Pomorskie, Lublin, Olsztyn, Opole, Piotrkow Trybunalski, Sosnowiec, Szczecin, Warsaw, Wroclaw and Zielona Gora. The volumetric method was applied using the Burkard or Lanzoni trap. The pollen season was determined by the 98% method. The season started earliest in Sosnowiec (April 14th). The mean duration of the pollen season was 33 days. The highest pollen concentration (713 P/m3) was observed in Wroclaw (April 19th). The peak values were recorded between April 19th and May 1st in the different cities