43 research outputs found

    Protein S100-A7 derived from digested dentin is a critical molecule for dentin pulp regeneration

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    Dentin consists of inorganic hard tissue and organic dentin matrix components (DMCs). Various kinds of bioactive molecules are included in DMCs and some of them can be released after digestion by endogenous matrix metalloproteinases (MMPs) in the caries region. Digested DMCs induced by MMP20 have been reported to promote pulpal wound healing processes, but the released critical molecules responsible for this phenomenon are unclear. Here, we identified protein S100-A7 as a critical molecule for pulpal healing in digested DMCs by comprehensive proteomic approaches and following pulp capping experiments in rat molars. In addition, immunohistochemical results indicated the specific distribution of S100-A7 and receptor for advanced glycation end-products (RAGE) as receptor for S100-A7 in the early stage of the pulpal healing process, and following accumulation of CD146-positive stem cells in wounded pulp. Our findings indicate that protein S100-A7 released from dentin by MMP20 might play a key role in dentin pulp regeneration

    C4b Binding Protein Binds to CD154 Preventing CD40 Mediated Cholangiocyte Apoptosis: A Novel Link between Complement and Epithelial Cell Survival

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    Activation of CD40 on hepatocytes and cholangiocytes is critical for amplifying Fas-mediated apoptosis in the human liver. C4b-Binding Protein (C4BP) has been reported to act as a potential surrogate ligand for CD40, suggesting that it could be involved in modulating liver epithelial cell survival. Using surface plasmon resonance (BiaCore) analysis supported by gel filtration we have shown that C4BP does not bind CD40, but it forms stable high molecular weight complexes with soluble CD40 ligand (sCD154). These C4BP/sCD154 complexes bound efficiently to immobilised CD40, but when applied to cholangiocytes they failed to induce apoptosis or proliferation or to activate NFkB, AP-1 or STAT 3, which are activated by sCD154 alone. Thus C4BP can modulate CD40/sCD154 interactions by presenting a high molecular weight multimeric sCD154/C4BP complex that suppresses critical intracellular signalling pathways, permitting cell survival without inducing proliferation. Immunohistochemistry demonstrated co-localisation and enhanced expression of C4BP and CD40 in human liver cancers. These findings suggest a novel pathway whereby components of the complement system and TNF ligands and receptors might be involved in modulating epithelial cell survival in chronic inflammation and malignant disease

    Critical micelle concentration and partition coefficient of mixed micelles: Analysis of ternary systems based on Markov chain model and simple mixture model

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    Hypothesis: When two or more surfactants are mixed, the mixed system exhibits improved performance compared with a single surfactant system in solution. The Markov chain model can analyze the critical micelle concentration (CMC) of mixed micelles, yielding results similar to those of a simple mixture model, which is typically referred to as the “regular solution theory.” In this study, two hypotheses were tested: (1) the Markov chain model for ternary systems can be simplified by approximating the association constant of surfactants i and j as Kij = Kji and (2) the quasi-simple ternary mixture model, that is, an analogous simple mixture model of the Markov chain model, helps interpret the interaction parameter of the simple mixture model that can describe the partition coefficient of the binary mixed micelle. Experiments: Equations were derived for (1) the Markov chain model for ternary systems by assuming Kij = Kji and (2) the interaction parameter of the simple mixture model that can describe the partition coefficient of the binary mixed micelle. Findings: The models were compared with the experiment data, and the derived equations described the experimental data of the CMC and partition coefficient well

    Joo gaeru to yumekochan

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    Tonchi kurabe ikkyusan

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    Boru no ponchan ; gin no suzu to nezumi

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