LOCALIZATION OF THE INF-1B INTERACTING SITE ON THE NCOA7-AS PROMOTER REGION

When treated with interferon-beta-1b, gene NCOA7 expresses a new variant that begins from an alternative start site NCAO7-AS with strong antioxidant properties. Segments of three different lengths of the upstream region of the gene were inserted into phRLnull. After testing and confirming their structure, the plasmids were transfected into mammalian cells. A luciferase assay was performed to determine which segments showed an increase in activity when treated with interferon. While each ligated plasmid acted as a strong promoter for Renilla luciferase, no significant difference in signal due to INF-beta- 1b treatment was observed

Enhancing the Effectiveness of the Santa Fe Bus System

This Interactive Qualifying Project dealt with improving the effectiveness of the Santa Fe Trails public bus system in Santa Fe, NM. Using on-board cameras, bus ridership data, traffic data, and survey response of bus system riders, our team was able to develop recommendations for bus system improvements such as rerouting several bus lines, and changing the bus schedules to create better connection times. To further effectiveness of the system we designed a mobile application, incorporating a Google Transit feed, to help passengers find buses and pay for their trips. It is our hope that these recommendations help the bus system become more convenient for riders of the bus system and encourage more people to ride the bus