A mesoporous ionic solid with 272 Auᴵ₆Agᴵ₃Cuᴵᴵ₃ complex cations in a super huge crystal lattice

Chem. Sci., 2021,12, 11045-11055

Gene Silencing Using 4′-thioDNA as an Artificial Template to Synthesize Short Hairpin RNA Without Inducing a Detectable Innate Immune Response

The development of a versatile technique to induce RNA interference (RNAi) without immune stimulation in vivo is of interest as existing approaches to trigger RNAi, such as small interfering RNA (siRNA) and plasmid DNA (pDNA) expressing short hairpin RNA (shRNA), present drawbacks arising from innate immune stimulation. To overcome them, an intelligent shRNA expression device (iRed) designed to induce RNAi was developed. The minimum sequence of iRed encodes only the U6 promoter and shRNA. A series of iRed comprises a polymerase chain reaction (PCR)-amplified 4′-thioDNA in which any one type of adenine (A), guanine (G), cytosine (C), or thymine (T) nucleotide unit was substituted by each cognate 4′-thio derivatives, i.e., dSA iRed, dSG iRed, dSC iRed, and ST iRed respectively. Each modified iRed acted as a template to transcribe shRNA with RNAi activity. The highest shRNA yield was generated using dSC iRed that exerted gene silencing activity in an orthotopic mouse model of mesothelioma. Reducing the minimal structure required to transcribe shRNA and the presence of the 4′-thiomodification synergistically function to abrogate innate immune response induced by dsDNA. The iRed will introduce a new approach to induce RNAi without inducing a detectable innate immune response

μ-Sulfido-bis{[1,1′-bis(diphenylphosphanyl)ferrocene-κ2P,P′]gold} methanol monosolvate

The gold complex in title compound, [Au2Fe(C17H14P)2S]·CH3OH, (I)·CH3OH, is closely similar to the previously reported (I)·2CHCl3, reported in the literature [Canales et al. (1996). J. Am. Chem. Soc. 118, 4839–4845]. Both crystallize in the monoclinic crystal systems but the space groups differ, P21/n for (I)·CH3OH compared to P2/n for (I)·2CHCl3. The two structures can be considered as polymorphs due to solvation differences. In (I)·CH3OH, all atoms of the methanol solvent molecule are disordered over two sets of sites with an occupancy ratio of 0.822 (12):0.178 (12). The crystal structure features O—H...S, C—H...S hydrogen bonds and C—H...π interactions that stack the complex molecules along the a-axis direction

Hexakis(<i>μ</i>-3-aminopropanethiolato-1κ⁶<i>N,S</i>:2κ³<i>S</i>;3κ⁶<i>N</i>,S:2κ³<i>S</i>)cadmium(II)dirhodium(III) Dibromide Tetrahydrate

Cadmium(II) complexes with thiolate ligands have received considerable attention because of their intriguing structural features and relevance to metalloproteins. In this study, a new cadmium(II)–rhodium(III) trinuclear complex, [Cd{Rh(apt)3}2]Br2·4H2O (1, apt = 3-aminopropanethiolate), was synthesized by the reaction of fac-[Rh(apt)3] with cadmium bromide. Compound 1 was characterized using elemental analysis, X-ray fluorescence and IR spectroscopies, and powder X-ray diffraction study. Single-crystal X-ray analysis revealed that the cadmium(II) center in 1 was surrounded by six thiolato S atoms from two fac-[Rh(apt)3] units

A Pseudorotaxane System Containing γ-Cyclodextrin Formed via Chiral Recognition with an Auᴵ₆Agᴵ₃Cuᴵᴵ₃ Molecular Cap

T. Kojima, H. Takeda, N. Kuwamura, T. Konno, Chem. Eur. J. 2021, 27, 15981