462 research outputs found
All-or-none switching of transcriptional activity on single DNA molecules caused by a discrete conformational transition
Recently, it has been confirmed that long duplex DNA molecules with sizes
larger than several tens of kilo-base pairs (kbp), exhibit a discrete
conformational transition from an elongated coil state to a compact globule
state upon the addition of various kinds of chemical species that usually
induce DNA condensation. In this study, we performed a single-molecule
observation on a large DNA, Lambda ZAP II DNA (ca. 41 kbp), in a solution
containing RNA polymerase and substrates along with spermine, a tetravalent
cation, at different concentrations, by use of fluorescence staining of both
DNA and RNA. We found that transcription, or RNA production, is completely
inhibited in the compact state, but is actively performed in the unfolded coil
state. Such an all-or-none effect on transcriptional activity induced by the
discrete conformational transition of single DNA molecules is discussed in
relation to the mechanism of the regulation of large-scale genetic activity.Comment: 14 pages, 2 figure
On the Disclosure of Accounting Policies : Attaching Importance to APB OPINION No. 22
故清水宗ー教授追悼
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