UNEXPECTED ECTOPIC BONE FORMATION ON THE DORSUM OF A FOOT AFTER FREE PERONEAL FLAP TRANSFER : A CASE REPORT

We experienced a case in which ectopic bone formation with trabecular structures occurred after free peroneal flap transfer containing periosteum of a fibula onto a soft tissue defect on the dorsum of a foot in a 2-year 9-month-old boy. In this case, ectopic bone formation was detected by radiography 5 months after the operation and the bone was removed because of the restricted dorsiflexion of the ankle. This case suggests that new bone can be formed by the periosteum if it is grafted with its vasculature preserved by microvascular anastomosis

RESULTS OF EATON AND LITTLER'S LIGAMENT RECONSTRUCTION FOR INSTABILITY OF THE FIRST CARPOMETACARPAL JOINT

This study presents the cases of Eaton and Littler's ligament reconstruction for the treatment of instability of lst CM joint resulting from early stage osteoarthritis (Group OA), trauma (Group T), and a part of generalized joint laxity (Group GJL) in 21 joints of 20 cases. Omitting six cases with less than one year follow-up, 15 joints in 14 cases were analyzed. Clinically, hypermobility pain was improved in all joints. However, eight out of 14 patients felt their joints stretched, but still had the normal range of motion. Grip power and pinch power was significantly improved (P＜0.01, P＜0.05). Comparing each group, only Group T revealed a significant difference between preoperative and postoper- ative grip power (P＜0.01). In increase of grip power, Group T were more significantly improved than Group OA (P＜0.01) and Group GJL (P＜0.05). Radiographically, postoper- ative stages of 4 joints in 4 cases out of 8 cases which felt stretched progressed from their preoperative stages. We assumed that the newly reconstructed ligament may be strong enough to maintain the stability of 1st CM joint and ligament reconstruction is most effective in treating post-traumatic instability

RESULTS OF EATON AND LITTLER'S LIGAMENT RECONSTRUCTION FOR INSTABILITY OF THE FIRST CARPOMETACARPAL JOINT

This study presents the cases of Eaton and Littler's ligament reconstruction for the treatment of instability of lst CM joint resulting from early stage osteoarthritis (Group OA), trauma (Group T), and a part of generalized joint laxity (Group GJL) in 21 joints of 20 cases. Omitting six cases with less than one year follow-up, 15 joints in 14 cases were analyzed. Clinically, hypermobility pain was improved in all joints. However, eight out of 14 patients felt their joints stretched, but still had the normal range of motion. Grip power and pinch power was significantly improved (P＜0.01, P＜0.05). Comparing each group, only Group T revealed a significant difference between preoperative and postoper- ative grip power (P＜0.01). In increase of grip power, Group T were more significantly improved than Group OA (P＜0.01) and Group GJL (P＜0.05). Radiographically, postoper- ative stages of 4 joints in 4 cases out of 8 cases which felt stretched progressed from their preoperative stages. We assumed that the newly reconstructed ligament may be strong enough to maintain the stability of 1st CM joint and ligament reconstruction is most effective in treating post-traumatic instability

iPSC-derived mesenchymal cells that support alveolar organoid development

肺胞オルガノイドをつくることができるヒトiPS細胞由来間葉細胞の作成. 京都大学プレスリリース. 2022-10-12.Mesenchymal cells are necessary for organ development. In the lung, distal tip fibroblasts contribute to alveolar and airway epithelial cell differentiation and homeostasis. Here, we report a method for generating human induced pluripotent stem cell (iPSC)-derived mesenchymal cells (iMESs) that can induce human iPSC-derived alveolar and airway epithelial lineages in organoids via epithelial-mesenchymal interaction, without the use of allogenic fetal lung fibroblasts. Through a transcriptome comparison of dermal and lung fibroblasts with their corresponding reprogrammed iPSC-derived iMESs, we found that iMESs had features of lung mesenchyme with the potential to induce alveolar type 2 (AT2) cells. Particularly, RSPO2 and RSPO3 expressed in iMESs directly contributed to AT2 cell induction during organoid formation. We demonstrated that the total iPSC-derived alveolar organoids were useful for characterizing responses to the influenza A (H1N1) virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, demonstrating their utility for disease modeling

Double chambered right ventricle with severe calcification of the tricuspid valve in an elderly woman: a case report

<p>Abstract</p> <p>Introduction</p> <p>Double chambered right ventricle is a rare congenital cardiac anomaly in which the right ventricle is divided into two chambers by an anomalous muscle bundle. The diagnosis of this disorder is difficult in adults. Calcification of the tricuspid valve is extremely rare, and very few cases have been reported. Most cases of tricuspid valve calcification had a congenital disorder with high pressure in the right ventricle.</p> <p>Case presentation</p> <p>We report a rare case of a 71-year-old Japanese woman who presented with chest discomfort, and was found to have a double chambered right ventricle with severe calcification of the tricuspid valve. This abnormality was found by echocardiography, and the diagnosis was confirmed by multislice cardiac computerized tomography, cardiac magnetic resonance imaging, and cardiac catheterization. Our patient rejected surgical repair, and medical therapy with carvedilol was effective to reduce her symptoms.</p> <p>Conclusion</p> <p>Calcification of the tricuspid valve is extremely rare, and considered to be due to high pressure in the right ventricle. To the best of our knowledge, there are no other reported cases of this combination of double chambered right ventricle and calcification of the tricuspid valve.</p

The AAA-ATPase VPS4 Regulates Extracellular Secretion and Lysosomal Targeting of α-Synuclein

Many neurodegenerative diseases share a common pathological feature: the deposition of amyloid-like fibrils composed of misfolded proteins. Emerging evidence suggests that these proteins may spread from cell-to-cell and encourage the propagation of neurodegeneration in a prion-like manner. Here, we demonstrated that α-synuclein (αSYN), a principal culprit for Lewy pathology in Parkinson's disease (PD), was present in endosomal compartments and detectably secreted into the extracellular milieu. Unlike prion protein, extracellular αSYN was mainly recovered in the supernatant fraction rather than in exosome-containing pellets from the neuronal culture medium and cerebrospinal fluid. Surprisingly, impaired biogenesis of multivesicular body (MVB), an organelle from which exosomes are derived, by dominant-negative mutant vacuolar protein sorting 4 (VPS4) not only interfered with lysosomal targeting of αSYN but facilitated αSYN secretion. The hypersecretion of αSYN in VPS4-defective cells was efficiently restored by the functional disruption of recycling endosome regulator Rab11a. Furthermore, both brainstem and cortical Lewy bodies in PD were found to be immunoreactive for VPS4. Thus, VPS4, a master regulator of MVB sorting, may serve as a determinant of lysosomal targeting or extracellular secretion of αSYN and thereby contribute to the intercellular propagation of Lewy pathology in PD