Effect of the type of application of Newcastle disease virus on the Ehrlich ascites tumor

Newcastle disease virus (NDV) has been shown to have an inhibitory effect on the tumours. Most authors use peritumoral application of virus. The purpose of our studz was to compare the effects of the ip in contrast to sc application of the virus on the ip and sc transplanted Ehrlich ascites tumor (EAT) in CBA/H mouse. We measured the length of survival, the tumor cure rates, the metastatic rate, and the frequencz of ascites and sc tumors in the site of ip EAT injection. Prolongation of survival after the therapy with NDV in ip transplanted EAT average time of survival in control group was 70.5 days, and 107 and 79.9 days with ip and sc NDV virus therapy respectively. The differences were significant only between control group and the group treated with ip application of NDV. Tumor cure rates were: ipNDV group 30%, scNDV group 20% and control group 5%. NDV therapy in sc transplanted EAT prolonged the time of survivalin control group it was 63.3 days, and 75.2 and 65.9 days with ip and sc NDV therapy respectively. NDV therapy inhibited metastatic rate of ip transplanted EAT. Inhibition was more effective with ip application of NDV. VIrus therapz also lowered the frequencz of appearance of ascites and sc tumour in the site of ip EAT injevtion. In sc transplanted EAT ip application of NDV inhibited the metastatic rate while in sc applied NDV some stimulation of metastasation was found. Ip application of NDV was found to be superior in contrast to sc application in all its therapeutic effects against EAT. Our results show that the tumor inhibition of NDV, in the system we used, has the characteristics of the biological response modifiers

Utjecaj poslijeoperacijske epiduralne analgezije na poslijeoperacijsku bol i stresni odgovor nakon veće operacije kralježnice – randomizirano kontrolirano dvostruko slijepo ispitivanje

Major spinal surgery is associated with severe postoperative pain and stress response, bowel dysfunction, and a potential for chronic pain development. Epidural analgesia has been shown to be advantageous compared to intravenous analgesia alone. The aim of the study was to investigate whether postoperative addition of epidural levobupivacaine to intravenous opioid analgesia offers advantage over intravenous opioid analgesia alone. Eighty-one patients scheduled for spinal fusion were enrolled in the study and randomized into two groups. Postoperatively, group A received 0.125% epidural levobupivacaine and group B received saline. Both groups also received intravenous piritramide as a rescue analgesic. Pain intensity, rescue analgesic consumption, blood glucose, cholesterol and cortisol levels, postoperative blood loss, paresthesia, time to first postoperative defecation, and length of hospital stay were recorded. Sixty-eight patients completed the study. The visual analog scale score (mean 2 vs. 4, p=0.01), consumption of piritramide (25 mg vs. 51.5 mg, p=0.01) and metamizole (1400 vs. 1875 mg, p<0.01), incidence of nausea (6% vs. 28% p=0.02) and blood loss (450 mL vs. 650 mL, p<0.05) were significantly lower in group A. Bowel recovery and first postoperative defecation also occurred earlier in group A (6% vs. 45%, p<0.01). Blood cortisol, glucose and cholesterol levels and the incidence of paresthesia did not differ between the groups. In conclusion, after spinal fusion, postoperative epidural administration of levobupivacaine provides better analgesia and fewer side effects with no impact on stress response.Cilj naše studije je bio usporediti poslijeoperacijsku epiduralnu analgeziju s intravenskom analgezijom piritramidom koju bolesnik kontrolira sam (patient controlled analgesia, PCA). Ocjenjivali smo poslijeoperacijske bolove i stresni odgovor. U ovu prospektivnu randomiziranu dvostruko slijepu studiju bio je uključen 81 bolesnik u razdoblju od srpnja 2007. Do studenoga 2010. godine. Bolesnici su bili podijeljeni u skupinu A koja je dobila levobupivakain kroz epiduralni kateter i skupinu B koja je istim putem dobivala fiziološku otopinu za poslijeoperacijsku analgeziju. Obje skupine su poslijeoperacijski dobile piritramid putem PCA pumpe. Poslijeoperacijski smo ocjenjivali bolove prema vizualno analognoj ljestvici (VAS), potrošnju analgetika, koncentraciju glukoze, kolesterola i kortizola u krvi, gubitak krvi, peristaltiku, razdoblje do prve defekacije i trajanje boravka u bolnici. Na kraju studije bilo je uključeno 68 bolesnika. Ocjena bolova prema VAS, potrošnja piritramida, metamizola, incidencija mučnine i gubitak krvi su bili značajno niži u skupini A (p<0,05). Peristaltika i prva poslijeoperacijska defekacija su se pojavile ranije u skupini A (p<0,01). Među skupinama nije bilo razlike u koncentraciji kortizola, kolesterola i glukoze u krvi. Nakon operacije kralježnice epiduralni levobupivakain je omogućio bolju poslijeoperacijsku analgeziju i manje nuspojava, manji gubitak krvi i raniji povratak crijevne funkcije u usporedbi s intravenskom analgezijom piritramidom