8 research outputs found
Activity of essential oils of three Micromeria species (Lamiaceae) against micromycetes and bacteria
The chemical composition of essential oils from three Micromeria species: M. dalmatica Benth., M. albanica (Griceb. ex K. Mal) Silic and M. thymifolia (Scop.) Fritsch were investigated by GC and GC-MS and their antibacterial and antifungal activities against seven fungal and six bacterial species were evaluated. Biological assays showed strong fungitoxicity of oils from all three Micromeria spp., particularly M. albanica, against all fungi tested. Essential oils of these species also exerted antibacterial effect against Streptococcus aureus, Escherichia coli, Bacillus subtilis and Micrococcus luteus at low concentrations. Higher concentrations of essential oil of M. albanica and M. dalmatica were active against Gram-negative Pseudomonas aeruginosa, which could be due to the high content of piperitenone oxide. Copyright (C) 2002 John Wiley Sons, Ltd.nul
Activity of essential oils of three Micromeria species (Lamiaceae) against micromycetes and bacteria
The chemical composition of essential oils from three Micromeria species: M. dalmatica Benth., M. albanica (Griceb. ex K. Mal) Silic and M. thymifolia (Scop.) Fritsch were investigated by GC and GC-MS and their antibacterial and antifungal activities against seven fungal and six bacterial species were evaluated. Biological assays showed strong fungitoxicity of oils from all three Micromeria spp., particularly M. albanica, against all fungi tested. Essential oils of these species also exerted antibacterial effect against Streptococcus aureus, Escherichia coli, Bacillus subtilis and Micrococcus luteus at low concentrations. Higher concentrations of essential oil of M. albanica and M. dalmatica were active against Gram-negative Pseudomonas aeruginosa, which could be due to the high content of piperitenone oxide. Copyright (C) 2002 John Wiley Sons, Ltd.nul
Antimutagenic effect of essential oil of sage (Salvia officinalis L.) and its fractions against UV-induced mutations in bacterial and yeast cells
The inhibition of spontaneous and UV-induced mutations by essential oil (EO) of sage (Salvia officinalis L.) and its fractions F1-F5 containing different proportions of mono- and sesquiterpenes was studied with the Salmonella/microsome, E. coli K12, and S. cerevisiae D7 reversion assays. The EO, F1, and F2 exhibited antimutagenic potential against UV-induced mutations in all tests. Fractions F3 and F4 produced a toxic, mutagenic, or antimutagenic response, depending on the test organism used. Reduction of spontaneous and UV-induced mutations by F5 was detected only in permeable strains of E. coli. The obtained results demonstrate antimutagenic activity of volatile sage terpenes and recommend them for further antimutagenesis and anticarcinogenesis studies
Comparative study on the antibacterial activity of volatiles from sage (Salvia officinalis L.)
Antibacterial activity of volatiles from sage against Gram-positive and Gram-negative bacteria from the ATCC collection was screened with the disk diffusion test. The essential oil and its fractions showed a significant antibacterial effect against S. aureus and B. subtilis. The minimum inhibitory concentrations were 1.25-2.5 μL/mL for S. aureus and 0.15-2.5 μL/mL for B. subtilis. The effect on S. aureus was bactericidal, while initial bactericidal effect on B. subtilis was impaired by the presence of a resistant fraction of the population, probably endospores. The results obtained with wild type and permeable strains of E. coli and S. typhimurium indicate that transport through the cell wall limits the antibacterial effect of sage volatiles
Libyan Thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties
AimsIn the present work, the Libyan wild-growing Thymus capitatus
essential oil (EO) was evaluated for its biological properties.
Methods and ResultsCarvacrol (6819\%) and thymol (1229\%) were found to
be the main compounds of the oil. Antioxidant properties, determined by
2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were
119, 403 and 105gml(-1) for oil, thymol and carvacrol respectively.
Microdilution method showed strong antibacterial and especially
antifungal potential. Tetrazolium (MTT) colorimetric assay indicated
moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29
(IC50=30-150gml(-1)). In adhesion-inhibition assay oil and main
compounds reduced adhesion of Escherichia coli and Listeria
monocytogenes on colon cells HT-29 (51 and 39\% of inhibition against
L.monocytogenes and E.coli respectively).
ConclusionsEssential oil of Th.capitatus showed moderate cytotoxic
activity, together with excellent antimicrobial effect, in particular
against fungi, and significant potential to reduce pathogen colonization
in colon.
Significance and Impact of the StudyThis is the first report that EO of
Th.capitatus could protect against colonization of pathogens to colon
epithelium. Thymus capitatus from Libya should be recognized as possible
new source of natural antioxidants, antimicrobials as well as possible
source of new chemotherapeutics.Ministry of Science of Republic of Serbia {[}173029, 173032, 172058