5 research outputs found
The cellular composition of the adrenal medulla of sexually mature guinea pigs
The adrenal glands from 15 guinea pigs aged 90 days were used in the study.
Paraffin slices were stained with Mayer haematoxylin and eosin with Masson
method and silvered. The histochemical reactions were carried out for catecholamines
and the biochemical determinations of catecholamines. Ultrastructural
observations were also performed.
In the adrenal medulla of sexually mature guinea pigs numerous epinephrocites
and no noreoinefrocites were observed when histochemical methods were used.
The small and large ganglion cells accompanying the supporting and neurolemmal
cells formed typical gangliar weaving (medullary ganglia). Apart from epinephrocytes,
small and large ganglion cells and supporting and neurolemmal
cells, small intensively fluorescent (SIF) cells were also detected.
Biochemical investigation revealed that the mean content of adrenaline calculated
for a pair of adrenal glands was 98.87% of the pool of catecholamines,
whereas the mean content of noradrenaline was 1.00%, and of dopamine 0.13%
Immunolocalization of CGRP, NPY and PGP 9.5 in guinea pig skin
By means of immunoperoxidase and immune-alkaline phosphatase methods the
immunoreactivities to neuropeptides: neuropeptide Y (NPY), calcitonin gene related
peptide (CGRP) and a pan-neuronal marker, the protein gene product 9.5 (PGP
9.5), were evaluated in guinea pig deep dorsal skin specimens. CGRP immunoreactive
(CGRP-IR) and NPY-immunoreactive (NPY-IR) nerve fibres were dispersed in
the papillary dermis and sometimes inside the hair roots and among the sebaceous
gland cells. Such localized nerve fibres have not so far been described. In the subcutaneous
layer nerve trunks were found composed of CGRP-IR and NPY-IR nerve
fibres. Some of these indicated vestigial or negative immunoreactivity to PGP 9.5