11 research outputs found
The Role of Serum Neutrophil Gelatinase-associated Lipocalin in the Early Diagnosis of Nephropathy in Patients with Acute Alcohol Poisoning
AIM: In our study, we assessed the possibility of using the serum neutrophil gelatinase-associated lipocalin (NGAL) for the early detection of kidney damage in patients with acute alcohol poisoning (AAP).
METHODS: The study included 89 patients and 30 healthy donors. All participants in the study were mostly represented by men (90%) aged between 20 and 40 years. The influence of alcohol poisoning severity was also taken into account in the study. The Human NGAL ELISA Kit was used for the quantitative detection of serum NGAL. We also evaluated the main laboratory indicators of kidney functions, including eGFR (calculated according to serum creatinine).
RESULTS: We did not find a correlation between blood alcohol concentration and serum NGAL level; also, alcohol poisoning severity did not affect the NGAL values. The results of our study showed the possibility of using the serum NGAL in patients with AAP to detect the preclinical stage of reduced renal function, until the moment when it can be diagnosed with using only serum creatinine.
CONCLUSION: We propose to consider an increase in eGFR together with an increase in serum NGAL in this group of patients as a stage, preceding nephropathy, even in the absence of clinical and laboratory signs of impaired renal function
Π₯ΠΈΠΌΠΈΠΊΠΎ-ΡΠΎΠΊΡΠΈΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΠΎΡΡΡΡΡ ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠΎΠΌ
Background. The relative availability of Phenazepam makes it a frequent cause of overdose, suicide and non-medical use. At the same time, it remains insufficiently studied in chemical and toxicological terms.The aim of study. to create an accessible, rapid method for detecting Phenazepam in biological matrices of patients with acute poisoning.Materials and methods. We used thin-layer chromatography (TLC), gas chromatography with a mass selective detector (GC-MS), high performance liquid chromatography with a tandem mass-selective detector (LC-MS/MS) and immunochromatographic analysis (ICA). The preparation of samples of intact urine with the addition of standard solutions of Phenazepam and real urine samples of patients with acute poisoning with Phenazepam was carried out using liquid-liquid extraction or precipitation of related components of the sample with acetonitrile. Hydrolysis and derivatization were also added in GC-MS analysis.Results. The analysis of statistics of the Department of Acute Poisonings of the N.V. Sklifosovsky Research Institute for Emergency Medicine in 2014-2016 showed that Phenazepam poisonings averaged 9.2% of the total number of admissions and mainly occurred as suicidal attempts. A technique has been developed for the detection of Phenazepam by TLC, which gives more objective results than ICA. For confirmatory analysis, it is advisable to use LC-MS/MS method for the native substance and GC-MS for the products of hydrolysis after derivatization. Compared to confirmatory methods, the developed TLC-screening technique is expressive, does not require the use of expensive high-tech equipment, difficult sample preparation, and makes it possible to reliably detect toxic and lethal concentrations of Phenazepam.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½Π°Ρ Π΄ΠΎΡΡΡΠΏΠ½ΠΎΡΡΡ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠ° Π΄Π΅Π»Π°Π΅Ρ Π΅Π³ΠΎ ΡΠ°ΡΡΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π΄ΠΎΠ·ΠΈΡΠΎΠ²ΠΎΠΊ, ΡΡΠΈΡΠΈΠ΄ΠΎΠ² ΠΈ Π½Π΅ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ. Π ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ ΠΎΠ½ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΈΠ·ΡΡΠ΅Π½Π½ΡΠΌ Π² Ρ
ΠΈΠΌΠΈΠΊΠΎΡΠΎΠΊΡΠΈΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ. Π¦ΠΠΠ¬ ΠΠ‘Π‘ΠΠΠΠΠΠΠΠΠ― Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π΄ΠΎΡΡΡΠΏΠ½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠ½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠ° Π² Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ΅Π΄Π°Ρ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΎΡΡΡΡΠΌ ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈΠΌ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠΎΠ½ΠΊΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ (Π’Π‘Π₯), Π³Π°Π·ΠΎΠ²ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Ρ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ (ΠΠ₯-ΠΠ‘), Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Ρ ΡΠ°Π½Π΄Π΅ΠΌΠ½ΡΠΌ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ (ΠΠΠΠ₯-ΠΠ‘/ΠΠ‘), ΠΈΠΌΠΌΡΠ½ΠΎΡ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· (ΠΠ₯Π). ΠΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠ° ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΈΠ½ΡΠ°ΠΊΡΠ½ΠΎΠΉ ΠΌΠΎΡΠΈ Ρ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
ΡΠ°ΡΡΠ²ΠΎΡΠΎΠ² ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠ° ΠΈ ΡΠ΅Π°Π»ΡΠ½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΌΠΎΡΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΎΡΡΡΡΠΌΠΈ ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΡΠΌΠΈ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠΎΠΌ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΆΠΈΠ΄ΠΊΠΎΡΡΡ-ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΡΡΡΠ°ΠΊΡΠΈΠΈ ΠΈΠ»ΠΈ ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΡ ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΏΡΠΎΠ±Ρ Π°ΡΠ΅ΡΠΎΠ½ΠΈΡΡΠΈΠ»ΠΎΠΌ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄ΠΎΠ±Π°Π²Π»ΡΠ»ΠΈ ΡΡΠ°Π΄ΠΈΠΈ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° ΠΈ Π΄Π΅ΡΠΈΠ²Π°ΡΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ₯-ΠΠ‘.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°ΡΠΈΡΡΠΈΠΊΠΈ ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΡ ΠΎΡΡΡΡΡ
ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΠΠΠ ΡΠΊΠΎΡΠΎΠΉ ΠΏΠΎΠΌΠΎΡΠΈ ΠΈΠΌ. Π.Π. Π‘ΠΊΠ»ΠΈΡΠΎΡΠΎΠ²ΡΠΊΠΎΠ³ΠΎ Π·Π° 2014β2016 Π³Π³. ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ ΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΡ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠΎΠΌ ΡΠΎΡΡΠ°Π²Π»ΡΡΡ Π² ΡΡΠ΅Π΄Π½Π΅ΠΌ 9,2% ΠΎΡ Π²ΡΠ΅Π³ΠΎ ΡΠΈΡΠ»Π° ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΠΉ ΠΈ ΡΠΎΠ²Π΅ΡΡΠ°ΡΡΡΡ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Ρ ΡΡΠΈΡΠΈΠ΄Π°Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π»ΡΡ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π’Π‘Π₯, ΠΊΠΎΡΠΎΡΠ°Ρ Π΄Π°Π΅Ρ Π±ΠΎΠ»Π΅Π΅ ΠΎΠ±ΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΡΠ΅ΠΌ ΠΠ₯Π. ΠΠ»Ρ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°ΡΡΠ΅Π³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ ΠΌΠ΅ΡΠΎΠ΄ ΠΠΠΠ₯-ΠΠ‘/ΠΠ‘ ΠΏΠΎ Π½Π°ΡΠΈΠ²Π½ΠΎΠΌΡ Π²Π΅ΡΠ΅ΡΡΠ²Ρ ΠΈ ΠΠ₯-ΠΠ‘ ΠΏΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°ΠΌ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° ΠΏΠΎΡΠ»Π΅ Π΄Π΅ΡΠΈΠ²Π°ΡΠΈΠ·Π°ΡΠΈΠΈ. ΠΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°ΡΡΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½Π°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° Π’Π‘Π₯-ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠ½ΠΎΡΡΡΡ, Π½Π΅ ΡΡΠ΅Π±ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄ΠΎΡΠΎΠ³ΠΎΡΡΠΎΡΡΠ΅Π³ΠΎ Π²ΡΡΠΎΠΊΠΎΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΡ, ΡΡΡΠ΄ΠΎΠ΅ΠΌΠΊΠΎΠΉ ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ²Π»ΡΡΡ ΡΠ΅Π½Π°Π·Π΅ΠΏΠ°ΠΌ Π² ΠΌΠΎΡΠ΅ Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ ΡΠ²ΡΡΠ΅ 1 ΠΌΠΊΠ³/ΠΌΠ» (ΠΏΡΠ΅Π΄Π΅Π» ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ)
Chemical and Toxicological Diagnosis of Acute Poisonings with Phenazepam
Background. The relative availability of Phenazepam makes it a frequent cause of overdose, suicide and non-medical use. At the same time, it remains insufficiently studied in chemical and toxicological terms.The aim of study. to create an accessible, rapid method for detecting Phenazepam in biological matrices of patients with acute poisoning.Materials and methods. We used thin-layer chromatography (TLC), gas chromatography with a mass selective detector (GC-MS), high performance liquid chromatography with a tandem mass-selective detector (LC-MS/MS) and immunochromatographic analysis (ICA). The preparation of samples of intact urine with the addition of standard solutions of Phenazepam and real urine samples of patients with acute poisoning with Phenazepam was carried out using liquid-liquid extraction or precipitation of related components of the sample with acetonitrile. Hydrolysis and derivatization were also added in GC-MS analysis.Results. The analysis of statistics of the Department of Acute Poisonings of the N.V. Sklifosovsky Research Institute for Emergency Medicine in 2014-2016 showed that Phenazepam poisonings averaged 9.2% of the total number of admissions and mainly occurred as suicidal attempts. A technique has been developed for the detection of Phenazepam by TLC, which gives more objective results than ICA. For confirmatory analysis, it is advisable to use LC-MS/MS method for the native substance and GC-MS for the products of hydrolysis after derivatization. Compared to confirmatory methods, the developed TLC-screening technique is expressive, does not require the use of expensive high-tech equipment, difficult sample preparation, and makes it possible to reliably detect toxic and lethal concentrations of Phenazepam
Effects of an Impeller Rim and Radial Clearance on Energy Characteristics of an Axial Pump
The results of numerical and experimental research conducted in the Laboratory for Hydraulic Machinery Construction of Peter the Great St. Petersburg Polytechnic University are presented. The research is aimed at studying the effects of an impeller radial clearance and rim on the energy characteristic of low-pressure axial pumps of the specific speed nsβ600. It is shown that these design features of a flow duct have significant effects on stage parameters, and they have to be accounted for when verifying design and experimental characteristics of axial pumps
The carboxy-terminal coiled-coil of the RNA polymerase Ξ²β²-subunit is the main binding site for Gre factors
Bacterial Gre transcript cleavage factors stimulate the intrinsic endonucleolytic activity of RNA polymerase (RNAP) to rescue stalled transcription complexes. They bind to RNAP and extend their coiled-coil (CC) domains to the catalytic centre through the secondary channel. Three existing models for the GreβRNAP complex postulate congruent mechanisms of Gre-assisted catalysis, while offering conflicting views of the GreβRNAP interactions. Here, we report the GreB structure of Escherichia coli. The GreB monomers form a triangle with the tip of the amino-terminal CC of one molecule trapped within the hydrophobic cavity of the carboxy-terminal domain of a second molecule. This arrangement suggests an analogous model for recruitment to RNAP. Indeed, the Ξ²β²-subunit CC located at the rim of the secondary channel has conserved hydrophobic residues at its tip. We show that substitutions of these residues and those in the GreB C-terminal domain cavity confer defects in GreB activity and binding to RNAP, and present a plausible model for the RNAPβGreB complex
Crystallization and preliminary crystallographic analysis of the transcriptional regulator RfaH from Escherichia coli and its complex with ops DNA
The E. coli transcriptional regulator RfaH was cloned, expressed, purified and crystallized and the complex of RfaH with its target DNA oligonucleotide was cocrystallized. Complete diffraction data sets were collected for the apo protein and its nucleic acid complex at 2.4 and at 1.6β
Γ
resolution, respectively