Metabolomic analyses of Leishmania reveal multiple species differences and large differences in amino acid metabolism

Comparative genomic analyses of Leishmania species have revealed relatively minor heterogeneity amongst recognised housekeeping genes and yet the species cause distinct infections and pathogenesis in their mammalian hosts. To gain greater information on the biochemical variation between species, and insights into possible metabolic mechanisms underpinning visceral and cutaneous leishmaniasis, we have undertaken in this study a comparative analysis of the metabolomes of promastigotes of L. donovani, L. major and L. mexicana. The analysis revealed 64 metabolites with confirmed identity differing 3-fold or more between the cell extracts of species, with 161 putatively identified metabolites differing similarly. Analysis of the media from cultures revealed an at least 3-fold difference in use or excretion of 43 metabolites of confirmed identity and 87 putatively identified metabolites that differed to a similar extent. Strikingly large differences were detected in their extent of amino acid use and metabolism, especially for tryptophan, aspartate, arginine and proline. Major pathways of tryptophan and arginine catabolism were shown to be to indole-3-lactate and arginic acid, respectively, which were excreted. The data presented provide clear evidence on the value of global metabolomic analyses in detecting species-specific metabolic features, thus application of this technology should be a major contributor to gaining greater understanding of how pathogens are adapted to infecting their hosts

Análise do movimento inicial de molares superiores submetidos a forças extrabucais: estudo 3D Analysis of initial movement of maxillary molars submitted to extraoral forces: a 3D study

OBJETIVO: analisar pelo método dos elementos finitos o deslocamento dos molares superiores frente a três diferentes inclinações do arco externo do aparelho extrabucal na tração do tipo cervical. MÉTODOS: maxila, dentes montados em má oclusão de Classe II e aparelho foram modelados através de formulação variacional e seus valores reproduzidos em coordenadas X, Y e Z. Foram realizadas simulações em microcomputador tipo PC, utilizando o programa ANSYS versão 8.1. Cada modelo de arco externo reproduziu linhas de força que passaram (1) acima (AcCR), (2) abaixo (AbCR) e (3) no centro de resistência (CR) do molar permanente superior de um mesmo modelo portador de má oclusão de Classe II. A avaliação restringiu-se ao movimento inicial dos molares frente à força extrabucal de 4 Newtons. RESULTADOS: o movimento distal inicial dos molares, tendo como ponto de referência a mesial do tubo, foi maior na coroa do modelo AbCR (0,47x10-6), e maior na raiz do modelo AcCR (0,32x10-6), provocando inclinações da coroa para distal e mesial, respectivamente. No modelo CR, os pontos na coroa (0,15x10-6) e raiz (0,12x10-6) deslocaram-se para distal equilibradamente, resultando em movimento de translação. Em todos os modelos, numa vista oclusal, houve tendência de rotação distal inicial da coroa, porém no modelo CR esse movimento foi muito pequeno. No sentido vertical (Z), todos os modelos revelaram movimento extrusivo (AbCR= 0,18x10-6; CR= 0,62x10-6; AcCR= 0,72x10-6). CONCLUSÃO: a simulação computacional do uso de aparelho extrabucal com tração cervical revelou a ocorrência de movimento extrusivo e distal, podendo ser por inclinação distal de coroa, de raiz ou movimento de translação.<br>OBJECTIVE: To analyze maxillary molar displacement by applying three different angulations to the outer bow of cervical-pull headgear, using the finite element method (FEM). METHODS: Maxilla, teeth set up in Class II malocclusion and equipment were modeled through variational formulation and their values represented in X, Y, Z coordinates. Simulations were performed using a PC computer and ANSYS software version 8.1. Each outer bow model reproduced force lines that ran above (ACR) (1), below (BCR) (2) and through the center of resistance (CR) (3) of the maxillary permanent molars of each Class II model. Evaluation was limited to the initial movement of molars submitted to an extraoral force of 4 Newtons. RESULTS: The initial distal movement of the molars, using as reference the mesial surface of the tube, was higher in the crown of the BCR model (0.47x10-6) as well as in the root of the ACR (0.32x10-6) model, causing the crown to tip distally and mesially, respectively. On the CR model, the points on the crown (0.15 x10-6) and root (0.12 x10-6) moved distally in a balanced manner, which resulted in bodily movement. In occlusal view, the crowns on all models showed a tendency towards initial distal rotation, but on the CR model this movement was very small. In the vertical direction (Z), all models displayed extrusive movement (BCR 0.18 x10-6; CR 0.62 x10-6; ACR 0.72x10-6). CONCLUSIONS: Computer simulations of cervical-pull headgear use disclosed the presence of extrusive and distal movement, distal crown and root tipping, or bodily movement

Efeitos do tratamento da Classe II divisão 1 em pacientes dolicofaciais tratados segundo a Terapia Bioprogressiva (AEB cervical e arco base inferior), com ênfase no controle vertical Treatment effects on Class II division 1 high angle patients treated according to the Bioprogressive therapy (cervical headgear and lower utility arch), with emphasis on vertical control

OBJETIVO: o presente estudo investigou o controle vertical e os efeitos do tratamento ortodôntico em pacientes dolicofaciais, empregando o AEB cervical e o arco base inferior. MÉTODOS: foi realizada a avaliação cefalométrica de 26 pacientes dolicofaciais com Classe II, divisão 1, idade média de 114 meses. O tratamento ortodôntico envolveu a utilização do AEB cervical na arcada superior e arco base na arcada inferior, até a obtenção da chave de oclusão normal dos molares, e finalizado segundo a Terapia Bioprogressiva, com duração média de 56 meses. Foram avaliados os valores de FMA, SN.GoGn, ANB, Fg-S, S-FPm, comprimento maxilar, comprimento mandibular, AFP (altura facial posterior), AFA (altura facial anterior), IAF (índice de altura facial), ângulo do plano oclusal, ângulo do plano palatino, QT (queixo total), LS (lábio superior) e ângulo Z. RESULTADOS: o tratamento promoveu estabilidade dos planos mandibular, oclusal e palatino. Ocorreu a correção anteroposterior das bases apicais, verificada pela redução significativa da grandeza ANB. A maxila apresentou um suave deslocamento anterior,com um suave aumento da dimensão anteroposterior.A mandíbula apresentou melhora de seu posicionamento em relação à base do crânio e sua dimensão anteroposterior aumentou significativamente. As alturas faciais posterior e anterior permaneceram em equilíbrio, não alterando significativamente o IAF. O perfil tegumentar apresentou melhora significativa. CONCLUSÃO: o tratamento realizado promoveu a correção das bases apicais, com controle dos planos horizontais e das alturas faciais, sendo efetivo no controle vertical.<br>OBJECTIVE: This study investigated vertical control and the effects of orthodontic treatment on dolichofacial patients, using cervical headgear (CHG) and lower utility arch. METHODS: Cephalometric assessment of 26 dolichofacial patients with Class II, division 1, and mean age of 114 months. Orthodontic treatment involved the use of cervical headgear (CHG) in the maxillary arch, lower utility arch in the mandibular arch until normal occlusion of the molars was obtained and finished in accordance with Bioprogressive therapy, with a mean duration of 56 months. The values of FMA, SN.GoGn, ANB, Fg-S, S-FPm, maxillary length, mandibular length, posterior facial height (PFH), anterior facial height (AFH), facial height index (FHI), occlusal plane angle (OPA), palatal plane angle (PPA), total chin (TC), upper lip (UL) and Z angle were evaluated. RESULTS: The results showed that treatment promoted stability of the mandibular, occlusal and palatal planes. Anteroposterior correction of the apical bases occurred, verified by the significant reduction in the variable ANB. The maxilla presented slight anterior displacement and increase in the anteroposterior dimension. The mandible presented improvement in its position in relation to the cranial base and its anteroposterior dimension increased significantly. The posterior and anterior facial heights remained in equilibrium, with no significant alteration in FHI. The tegumental profile presented significant improvement. CONCLUSION: The treatment performed produced correction of the apical basis with control of the horizontal planes and facial heights, and was effective for vertical control