Analysis of actual models of cooperation in regional innovative system

The article deals with models of collaboration. It is vital to note that there is no single model (example) for networking in regional innovative system, but most of existing models based on Triple Helix Model. In article also are described the common type of cooperation in network structure and detailed their main characteristics

Identification of regulatory variants associated with genetic susceptibility to meningococcal disease.

Non-coding genetic variants play an important role in driving susceptibility to complex diseases but their characterization remains challenging. Here, we employed a novel approach to interrogate the genetic risk of such polymorphisms in a more systematic way by targeting specific regulatory regions relevant for the phenotype studied. We applied this method to meningococcal disease susceptibility, using the DNA binding pattern of RELA - a NF-kB subunit, master regulator of the response to infection - under bacterial stimuli in nasopharyngeal epithelial cells. We designed a custom panel to cover these RELA binding sites and used it for targeted sequencing in cases and controls. Variant calling and association analysis were performed followed by validation of candidate polymorphisms by genotyping in three independent cohorts. We identified two new polymorphisms, rs4823231 and rs11913168, showing signs of association with meningococcal disease susceptibility. In addition, using our genomic data as well as publicly available resources, we found evidences for these SNPs to have potential regulatory effects on ATXN10 and LIF genes respectively. The variants and related candidate genes are relevant for infectious diseases and may have important contribution for meningococcal disease pathology. Finally, we described a novel genetic association approach that could be applied to other phenotypes

Estructura de vida del hombre adulto joven de clase socioeconómica baja, habitante de la zona Sabana centro de Cundinamarca

335 Páginas.Este estudio tuvo como objetivo caracterizar la estructura y reestructuración de vida del hombre adulto joven de clase socioeconómica baja y habitante de la zona Sabana centro de Cundinamarca. Es una investigación cualitativa. Utilizando el análisis del discurso, se interpretaron los textos de 6 historias de vida de hombres adultos jóvenes. Se establecieron 4 categorías de análisis, para el desarrollo adulto joven : intimidad, desarrollo laboral, sueños y amistades. Se encontró dentro de una cultura que aún sigue siendo machista, un hombre que fundamenta su identidad de género en su capacidad como proveedor de la familia. Un hombre, quien reconoce el valor de la educación y piensa en el futuro de sus hijos y en el propi

62nd Annual High Blood Pressure Conference 2008: Candidate Genes for 2-Methoxyestradiol-Mediated Vasoprotective Actions in Human Aortic Smooth Muscle Cells

Methoxyestradiol (2-ME), an endogenous metabolite of estradiol, not only exerts cytotoxic effects on cancer cells but also protects against multiple proliferative disorders, including atherosclerosis and injury-induced intimal thickening. However, the molecular mechanisms by which 2-ME induces its anti-vasoocclusive actions are largely unknown. Given that abnormal growth and excessive proliferation of smooth muscle cells (SMCs) importantly contribute to the vascular remodeling process associated with hypertension and atherosclerosis, the aims of this study were to assess the impact of 2-ME on pathophysiological pathways regulating SMC growth using transcriptional profiling. High-density oligonucleotide microarrays (Affymetrix Human Genome U_133 Plus 2.0 GeneChips) were used to identify differentially expressed genes in cultured human aortic SMCs treated with 2-ME (acutely, for 4 hrs, n=3; and chronically, for 48 hrs, n=3) and vehicle-treated time-matched controls (n=3 for each time point). Both single gene analysis (performed using Significance Analysis of Microarrays) as well as Gene Set Enrichment Analysis (GSEA, a computational method that determines whether an a priori defined set of genes shows statistically significant, concordant differences between two biological states) indicated downregulation of genes critically involved in mitotic spindle assembly and function in SMCs chronically treated with 2-ME when compared to control cultures. GSEA analysis identified significant effects of 2-ME on cell cycle progression, cell migration/adhesion, vasorelaxation, apoptosis, the inflammatory response, and cholesterol homeostasis/metabolism. Major changes were also observed in genes regulating the respiratory chain and redox pathways. Identification of candidate genes that are positively or negatively regulated by 2-ME provides important leads to investigate and better understand the mechanisms by which 2-ME induces its vasoprotective and antivasoocclusive actions

Control of Listeria monocytogenes infection requires classical IL-6 signaling in myeloid cells.

IL-6 is required for the response of mice against Listeria monocytogenes. Control of infection depends on classical IL-6 signaling via membrane IL-6Rα, but IL-6 target cells and protective mechanisms remain unclear. We used mice with IL-6Rα-deficiency in T cells (Il6rafl/fl×CD4cre) or myeloid cells (Il6rafl/fl×LysMcre) to define the role of these cells in IL-6-mediated protection. Abrogation of IL-6Rα in T cells did not interfere with bacteria control and induction of TH1 and CD8+ T-cell responses. IL-6Rα-deficiency in myeloid cells caused significant defects in listeria control. This defect was not associated with reduced recruitment of granulocytes and inflammatory monocytes, and both cell populations were activated and not impaired in cytokine production. However, IL-6Rα-deficient inflammatory monocytes displayed diminished expression of IL-4Rα and of CD38, a protein required for phagocytosis and innate control of listeria. In vitro studies revealed that IL-4 and IL-6 cooperated in induction of CD38. In listeria-infected mice, phagocytic activity of inflammatory monocytes correlated with CD38 expression levels on cells and inflammatory monocytes of Il6rafl/fl×LysMcre mice were significantly impaired in phagocytosis. In conclusion, we demonstrate that inhibition of classical IL-6 signaling in myeloid cells causes alterations in differentiation and function of these cells, which subsequently prevent effective control of L. monocytogenes