Small Non-Coding RNAs in Regulation of Course and Therapeutic Efficacy in Acute Myeloid Leukemia

Small non-coding RNAs (sncRNAs) are small regulatory molecules, which play key roles in fine-tune of all cell functions. In late 1970s and early 1980s, it was first determined that non-coding RNAs contribute to the cellular regulatory processes. The kingdom of sncRNAs is very numerous and it is clear that functions of different members of this family is different from each other and may be involved in normal and pathologic processes in cell. Recently it was investigated that sncRNAs and long non-coding RNAs play roles in cellular differentiation, proliferation, metabolic processes, bioenergetic regulation, cell death and inter-cellular communications, etc. In embryos, non-coding RNAs control maternal-zygotic transition, the maintenance of pluripotency, the pattering of the body axes, the specification and differentiation of cell types and morphogenesis of organs. Development of hematologic malignancies in humans, their course and regulation of resistance and sensitivity of tumorous cells to therapy are under the control of sncRNAs

Russian Military Personnel Under Institutional Reforms: Professional Attitude and Identity

Russian military reform influences conditions and mechanisms of professionalization of the servicemen's consciousness, their values and labor strategies. Multiple increase in the personnel wages, benefits for improving housing conditions, medical care, etc., allow the military to claim a special social position compared with the civilian population. Military reforms expand the opportunities for social mobility in the army, and the profession of the serviceman becomes again a competitive one. The research findings of the mass survey of 470 servicemen in Moscow and 256 servicemen in Rostov-on-Don, complemented by in-depth interviews with 15 officers demonstrate a positive trend in social status military positions in Russia. At present, among servicemen living in major cities, pragmatic orientations prevail, combined with a set of specific values (military duty, patriotism, partnership, attitude to one's profession as a calling), which form the basis of military-corporate identity. Dynasties (mainly relatives from military families are recruited) and the possibility of social mobility, which attracts representatives of low-income families and residents of rural areas, are the mechanisms of strengthening the corporate professional identity. The research findings indicate a greater degree of professionalization of the Moscow military personnel’s consciousness and the growth of pragmatic orientations in their work, a neutral prestige assessment of the military profession and a higher satisfaction with the quality of their lives. Professional identity is important, but not a prior component of their self-determination. For the Rostov servicemen, civil-patriotic and material-monetary parameters of professional activity are more significant indicators. Military identity is an axial characteristic of their identity. However, the claims about the significance of their work do not coincide with the assessment of their socio-economic security for the Rostov military personnel. Thus, the transition to the professional army model implies a shift from the heroic and patriotic foundations of the servicemen's identity to the growth of rational professional orientations. In the capital of Russia, this vector of transformation is more expressed than in the South-Russian region. Thus, in the face of the economic crisis and unresolved issues of army's modernization, the expectations for the privileged position of professional soldiers are inadequate for their real socioeconomic support

Small non-coding RNAs as regulators of structural evolution and carcinogenesis

Small non-coding RNAs (sncRNAs) are part of non-coding oligonucleotide regulators with wide physiologic and morphologic functions. They control genetic programing of cells, and may modulate processes of differentiation and death. Biogenesis of sncRNAs is now known, and some sncRNAs have been proposed as markers of malignization. Epigenetic therapy is based on the use of newly discovered genetic modifiers, such as sncRNAs, micro-RNAs, and theirs mimics. However, role of sncRNAs in structural evolution and mechanisms of adaptation is not clearly understood. Certainly, non-coding RNAs participate in processes of cellular and organismal adaptation as well as cellular and tissue structural transformation as response to changing of environmental neighbouring. Investigations into these functions of sncRNAs may be the basis of future epigenetic environmental medicine. Keywords: Small non-coding RNAs, Transposable elements, Structural evolution, Carcinogenesi

The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs

Background: Virus-induced cellular genetic modifications result in the development of many human cancers. Methods: In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously in the interscapular region with RVP3 cells. Three groups of mice were used. For treatment, one and/or two intravenous injections of a complex of small non-coding RNAs (sncRNAs) a-miR-155, piR-30074, and miR-125b with a 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC) delivery system were used. The first group consisted of untreated animals (control). The second group was treated with one injection of complex DDMC/sncRNAs (1st group). The third group was treated with two injections of complex DDMC/sncRNAs (2nd group). The tumors were removed aseptically, freed of necrotic material, and used with spleen and lungs for subsequent RT-PCR and immunofluorescence experiments, or stained with Leishman-Romanowski dye. Results: As a result, the mice fully recovered from virus-induced sarcoma after two treatments with a complex including the DDMC vector and a-miR-155, piR-30074, and miR-125b. In vitro studies showed genetic and morphological transformations of murine cancer cells after the injections. Conclusions: Treatment of virus-induced sarcoma of mice with a-miR-155, piR-30074, and miR-125b as active component of anti-cancer complex and DDMC vector as delivery system due to epigenetic-regulated transformation of cancer cells into cells with non-cancerous physiology and morphology and full recovery of disease. Keywords: Small non-coding RNAs, Src tyrosine kinase, Epigenetic therapy, Mice, DDMC vector, Sarcom

The Professional Identity of Urban Teachers in the Context of Educational Modernization

The paper deals with the research findings of the empirical study in secondary schools in Moscow and Rostov-on-Don in 2017. A survey of more than 1,500 respondents was aimed at analyzing the perception of their social and economic situation by the teachers; studying the teacher’s social identity structure; identifying the labor motivation and estimate the degree of precaritization of the Russian teachers. The survey findings have shown that the state social policy and salary level affect professional well-being of teachers. Teachers in the provincial city, compared with those who live in the capital (considering significant pay gap), are more critical about the prestige of their profession, quality of life and material prosperity, they are more sensitive to increased workload, without any noticeable increase in wage. At the same time, there are noticeably fewer Moscow teachers who are satisfied with the pedagogical profession itself. They are also concerned about the risks of job loss, wage cuts and increased workload. In the structure of teacher's group identity in Moscow and Rostov schools, professional groups are inferior to primordial and abstract-symbolic communities. At the same time, a comparative analysis of the survey data in different years indicates that teachers in Russia traditionally maintain high level of corporate professional identity, i.e. their commitment and loyalty to their place of work and their colleagues. In general, the excessive administrative burdens imposed on the teaching staff, as well as the societal demand for the results of teachers’ work in the context of the deteriorating economic situation in the country, create risks for maintaining positive professional identity of teachers, and largely limits the capacity of the reforms in school

Transverse momentum and pseudorapidity distributions of charged hadrons in pp collisions at (s)\sqrt(s) = 0.9 and 2.36 TeV

Measurements of inclusive charged-hadron transverse-momentum and pseudorapidity distributions are presented for proton-proton collisions at sqrt(s) = 0.9 and 2.36 TeV. The data were collected with the CMS detector during the LHC commissioning in December 2009. For non-single-diffractive interactions, the average charged-hadron transverse momentum is measured to be 0.46 +/- 0.01 (stat.) +/- 0.01 (syst.) GeV/c at 0.9 TeV and 0.50 +/- 0.01 (stat.) +/- 0.01 (syst.) GeV/c at 2.36 TeV, for pseudorapidities between -2.4 and +2.4. At these energies, the measured pseudorapidity densities in the central region, dN(charged)/d(eta) for |eta| < 0.5, are 3.48 +/- 0.02 (stat.) +/- 0.13 (syst.) and 4.47 +/- 0.04 (stat.) +/- 0.16 (syst.), respectively. The results at 0.9 TeV are in agreement with previous measurements and confirm the expectation of near equal hadron production in p-pbar and pp collisions. The results at 2.36 TeV represent the highest-energy measurements at a particle collider to date

Measurement of the charge ratio of atmospheric muons with the CMS detector

We present a measurement of the ratio of positive to negative muon fluxes from cosmic ray interactions in the atmosphere, using data collected by the CMS detector both at ground level and in the underground experimental cavern at the CERN LHC. Muons were detected in the momentum range from 5 GeV/ c to 1 TeV/ c . The surface flux ratio is measured to be 1.2766±0.0032(stat.)±0.0032(syst.) , independent of the muon momentum, below 100 GeV/ c . This is the most precise measurement to date. At higher momenta the data are consistent with an increase of the charge ratio, in agreement with cosmic ray shower models and compatible with previous measurements by deep-underground experiments.We present a measurement of the ratio of positive to negative muon fluxes from cosmic ray interactions in the atmosphere, using data collected by the CMS detector both at ground level and in the underground experimental cavern at the CERN LHC. Muons were detected in the momentum range from 5 GeV/c to 1 TeV/c. The surface flux ratio is measured to be 1.2766 \pm 0.0032(stat.) \pm 0.0032 (syst.), independent of the muon momentum, below 100 GeV/c. This is the most precise measurement to date. At higher momenta the data are consistent with an increase of the charge ratio, in agreement with cosmic ray shower models and compatible with previous measurements by deep-underground experiments

Transverse-momentum and pseudorapidity distributions of charged hadrons in pppp collisions at s\sqrt{s} = 7 TeV

Charged-hadron transverse-momentum and pseudorapidity distributions in proton-proton collisions at $\sqrt{s} = 7$~TeV are measured with the inner tracking system of the CMS detector at the LHC. The charged-hadron yield is obtained by counting the number of reconstructed hits, hit-pairs, and fully reconstructed charged-particle tracks. The combination of the three methods gives a charged-particle multiplicity per unit of pseudorapidity \dnchdeta|_{|\eta| < 0.5} = 5.78\pm 0.01\stat\pm 0.23\syst for non-single-diffractive events, higher than predicted by commonly used models. The relative increase in charged-particle multiplicity from $\sqrt{s} = 0.9$ to 7~TeV is 66.1\%\pm 1.0\%\stat\pm 4.2\%\syst. The mean transverse momentum is measured to be 0.545\pm 0.005\stat\pm 0.015\syst\GeVc. The results are compared with similar measurements at lower energies.Charged-hadron transverse-momentum and pseudorapidity distributions in proton-proton collisions at sqrt(s) = 7 TeV are measured with the inner tracking system of the CMS detector at the LHC. The charged-hadron yield is obtained by counting the number of reconstructed hits, hit-pairs, and fully reconstructed charged-particle tracks. The combination of the three methods gives a charged-particle multiplicity per unit of pseudorapidity, dN(charged)/d(eta), for |eta| < 0.5, of 5.78 +/- 0.01 (stat) +/- 0.23 (syst) for non-single-diffractive events, higher than predicted by commonly used models. The relative increase in charged-particle multiplicity from sqrt(s) = 0.9 to 7 TeV is 66.1% +/- 1.0% (stat) +/- 4.2% (syst). The mean transverse momentum is measured to be 0.545 +/- 0.005 (stat) +/- 0.015 (syst) GeV/c. The results are compared with similar measurements at lower energies