25 research outputs found
Effective affinities in microarray data
In the past couple of years several studies have shown that hybridization in
Affymetrix DNA microarrays can be rather well understood on the basis of simple
models of physical chemistry. In the majority of the cases a Langmuir isotherm
was used to fit experimental data. Although there is a general consensus about
this approach, some discrepancies between different studies are evident. For
instance, some authors have fitted the hybridization affinities from the
microarray fluorescent intensities, while others used affinities obtained from
melting experiments in solution. The former approach yields fitted affinities
that at first sight are only partially consistent with solution values. In this
paper we show that this discrepancy exists only superficially: a sufficiently
complete model provides effective affinities which are fully consistent with
those fitted to experimental data. This link provides new insight on the
relevant processes underlying the functioning of DNA microarrays.Comment: 8 pages, 6 figure
Can we improve outcome of congenital diaphragmatic hernia?
This review gives an overview of the disease spectrum of congenital diaphragmatic hernia (CDH). Etiological factors, prenatal predictors of survival, new treatment strategies and long-term morbidity are described. Early recognition of problems and improvement of treatment strategies in CDH patients may increase survival and prevent secondary morbidity. Multidisciplinary healthcare is necessary to improve healthcare for CDH patients. Absence of international therapy guidelines, lack of evidence of many therapeutic modalities and the relative low number of CDH patients calls for cooperation between centers with an expertise in the treatment of CDH patients. The international CDH Euro-Consortium is an example of such a collaborative network, which enhances exchange of knowledge, future research and development of treatment protocols
A New Strategy toward Indole Alkaloids Involving an Intramolecular Cycloaddition/Rearrangement Cascade
Mechanism of Addition of Organocuprates to Alkynyl Carbonyl Compounds. A Mechanistic Bridge between Carbocupration and Conjugate Addition
A standardized kodecyte method to quantify ABO antibodies in undiluted plasma of patients before ABO‐incompatible kidney transplantation
Stereocontrolled Syntheses of the Nemorensic Acids Using 6-Diazoheptane-2,5-dione in Carbonyl Ylide Cycloadditions
David M. Hodgson, Frédéric Le Strat, Thomas D. Avery, Andrew C. Donohue, and Tobias Brück