The Impact of Peroxisomes on Cellular Aging and Death

Peroxisomes are ubiquitous eukaryotic organelles, which perform a plethora of functions including hydrogen peroxide metabolism and β-oxidation of fatty acids. Reactive oxygen species produced by peroxisomes are a major contributing factor to cellular oxidative stress, which is supposed to significantly accelerate aging and cell death according to the free radical theory of aging. However, relative to mitochondria, the role of the other oxidative organelles, the peroxisomes, in these degenerative pathways has not been extensively investigated. In this contribution we discuss our current knowledge on the role of peroxisomes in aging and cell death, with focus on studies performed in yeast

Alcohol oxidase in Hansenula polymorpha : assembly, activation and inactivation of a peroxisomal enzyme

limited number of yeast species is capable of utilizing methalolas the sole carbon and energy source for growth. In these yeasts, the first step in methanol metabolism is catalyzed by the enzfrme alcohol oxidase (AO). This enzyme oxidizes methanof, using oxygen as electron acceptor, into formaldehyde and hydrogen peroxide. In its active form AO consists of eight identical subunits, each of which contains one fl-avin adenine dinucleotide (FAD) molecule as the prosthetic group. ... Zie: Summary

Improved penicillin production

The invention relates to improved means and methods for producing a compound of interest in fungal host cells. Provided is a method for producing at least one secondary metabolite in a fungal host cell, comprising culturing a fungal host cell capable of producing said metabolite under conditions allowing for production of said metabolite, wherein the host cell has been modified to display elevated levels of glyoxalase I and glyoxalase II activity

Improved penicillin production

The invention relates to improved means and methods for producing a compound of interest in fungal host cells. Provided is a method for producing at least one secondary metabolite in a fungal host cell, comprising culturing a fungal host cell capable of producing said metabolite under conditions allowing for production of said metabolite, wherein the host cell has been modified to display elevated levels of glyoxalase I and glyoxalase II activity

Improved penicillin production

The invention relates to improved means and methods for producing a compound of interest in fungal host cells. Provided is a method for producing at least one secondary metabolite in a fungal host cell, comprising culturing a fungal host cell capable of producing said metabolite under conditions allowing for production of said metabolite, wherein the host cell has been modified to display elevated levels of glyoxalase I and glyoxalase II activity