Chlamydia pneumoniae Hides inside Apoptotic Neutrophils to Silently Infect and Propagate in Macrophages

BACKGROUND: Intracellular pathogens have developed elaborate strategies for silent infection of preferred host cells. Chlamydia pneumoniae is a common pathogen in acute infections of the respiratory tract (e.g. pneumonia) and associated with chronic lung sequelae in adults and children. Within the lung, alveolar macrophages and polymorph nuclear neutrophils (PMN) are the first line of defense against bacteria, but also preferred host phagocytes of chlamydiae. METHODOLOGY/PRINCIPAL FINDINGS: We could show that C. pneumoniae easily infect and hide inside neutrophil granulocytes until these cells become apoptotic and are subsequently taken up by macrophages. C. pneumoniae infection of macrophages via apoptotic PMN results in enhanced replicative activity of chlamydiae when compared to direct infection of macrophages, which results in persistence of the pathogen. Inhibition of the apoptotic recognition of C. pneumoniae infected PMN using PS- masking Annexin A5 significantly lowered the transmission of chlamydial infection to macrophages. Transfer of apoptotic C. pneumoniae infected PMN to macrophages resulted in an increased TGF-ss production, whereas direct infection of macrophages with chlamydiae was characterized by an enhanced TNF-alpha response. CONCLUSIONS/SIGNIFICANCE: Taken together, our data suggest that C. pneumoniae uses neutrophil granulocytes to be silently taken up by long-lived macrophages, which allows for efficient propagation and immune protection within the human host

Transmission Line Modeling and Asymptotic Formulas for Periodic Leaky-Wave Antennas Scanning Through Broadside

Abstract-It is shown, using three specific examples-a series fed patch (SFP) array, a phase reversal (PR) array and a composite right/left-handed (CRLH) antenna-that one-dimensional periodic leaky-wave antennas scanning through broadside build a class of leaky-wave antennas sharing qualitatively similar and quantitatively distinct dispersion and radiation characteristics. Based on an equivalent transmission line (TL) model using linearized series and shunt immittances to approximate the periodic (Bloch) antenna structure, asymptotic TL formulas for the characteristic propagation constant, impedance, energy, power and quality factor are derived for two fundamentally different nearand off-broadside radiation regimes. Based on these formulas, it is established that the total powers in the series and shunt elements are always equal at broadside, which constitutes one of the central results of this contribution. This equal power splitting implies a severe degradation of broadside radiation when only one of the two elements series or shunt efficiently contributes to radiation and the other is mainly dissipative. A condition for optimum broadside radiation is subsequently established and shown to be identical to the Heaviside condition for distortionless propagation in TL theory. Closed-form expressions are derived for the constitutive (LCRG) parameters of the TL model for the specific SFP, PR and CRLH antenna circuit models, and quantitative information on the validity range of the TL model is subsequently provided. Finally, full-wave simulation and measurement LCRG parameter extraction methods are proposed and validated. Index Terms-Bloch-Floquet theorem, broadside radiation, composite right/left-handed (CRLH) metamaterial, Heaviside condition, leaky-wave antennas, periodic structures, phase-reversal (PR) array, quality factor, series-fed patch (SFP) array, transmission line (TL) theory

Cyclotrons Operated for Nuclear Medicine and Radiopharmacy in the German Speaking D-A-CH Countries: An Update on Current Status and Trends

Background: Cyclotrons form a central infrastructure and are a resource of medical radionuclides for the development of new radiotracers as well as the production and supply of clinically established radiopharmaceuticals for patient care in nuclear medicine. Aim: To provide an updated overview of the number and characteristics of cyclotrons that are currently in use within radiopharmaceutical sciences and for the development of radiopharmaceuticals to be used for patient care in Nuclear Medicine in Germany (D), Austria (A) and Switzerland (CH). Methods: Publicly available information on the cyclotron infrastructure was (i) consolidated and updated, (ii) supplemented by selective desktop research and, last but not least, (iii) validated by members of the committee of the academic “Working Group Radiochemistry and Radiopharmacy” (AGRR), consisting of radiochemists and radiopharmacists of the D-A-CH countries and belonging to the German Society of Nuclear Medicine (DGN), as well as the Radiopharmaceuticals Committee of the DGN. Results: In total, 42 cyclotrons were identified that are currently being operated for medical radionuclide production for imaging and therapy in Nuclear Medicine clinics, 32 of them in Germany, 4 in Austria and 6 in Switzerland. Two thirds of the cyclotrons reported (67%) are operated by universities, university hospitals or research institutions close to a university hospital, less by/in cooperation with industrial partners (29%) or a non-academic clinic/ PET-center (5%). Most of the cyclotrons (88%) are running with up to 18 MeV proton beams, which is sufficient for the production of the currently most common cyclotron-based radionuclides for PET imaging. Discussion: The data presented provide an academically-updated overview of the medical cyclotrons operated for the production of radiopharmaceuticals and their use in Nuclear Medicine in the D-A-CH countries. In this context, we discuss current developments and trends with a view to the cyclotron infrastructure in these countries, with a specific focus on organizational aspects

Four-Square Phased Array for Multibeam Applications using Novel Matrix Feed

Abstract -Modern communications systems use multi-beam antennas for the sectorization of 360° azimuthal coverage or diversity and MIMO concepts. One suitable antenna realization is based on the phased array principle, where four elements placed at the edges of a square are required in order to form four overlapping beams in azimuth. In its basic form, excitation phases of 0°, 90° and 180° are required for beam forming. The paper presents a novel multi-beam matrix network created by four 90°-hybrid couplers which realizes the required excitation for four beams spaced by 90° in the azimuth plane. A proof-ofconcept antenna was designed which employs miniature surfacemount couplers in a microstrip feed network and uses vertical monopole radiators