Morandin et al. Vg sequences

This file contains Vitellogenin and Vitellogenin-like sequences (amino acid and predicted protein) used for the phylogenetic tree (Figure 1), annotated in insect species for Morandin et al. 2014

Morandin et al. FexsVgs alignment

This file contains alignment generated via ClustalX of Vitellogenin and Vitellogenin-like sequences of Formica exsecta

Sequences matching microbes, and a mite, associated with <i>F. exsecta</i>, identified from GenBank BLAST searches.

<p>Total expression value represents the combined expression value for all ages and castes, from both FIMM and BGI data.</p><p>Intracellular microbes are known parasites or have at least one stage of their lifecycle inside the host. Extracellular microbes do not usually have lifecycles within hosts, but can in some cases be opportunistic pathogens. Unknown is used for yeast, these are pervasive in environments that contain moisture and carbohydrate sources, and may form symbiotic relationships with ants in their guts, or they can grow in the nest material, or be carried in via aphid dew that ants feed on. IAPV = Israel Acute Paralysis Virus, Acute Bee Paralysis Virus, KBV = Kashmir Bee Virus, DVW = deformed wing virus.</p>*<p>current classification.</p>**<p>Some <i>Kluyveromyces</i> are teleomorphs of <i>Candida.</i></p>∧<p>Fungi that gave hits on both common and species specific genes in GenBank.</p

Sequences matching eukaryotes of non-fungal origin identified from the 18s rRNA gene.

<p>GenBank match denotes the GenBank accession number of the closest GenBank BLAST match. Total expression value represents the combined expression value for all ages and castes, from both FIMM and BGI data.</p

Phylogeny of the available genomes of the insect-specific <i>Discistroviridae</i> and <i>Iridoviridae</i> from GenBank.

<p>Social insect viruses are highlighted in blue and ant-derived assembled virus genomes from this study are highlighted in red.</p

Phylogenetic tree of bacterial 16S rRNA sequences, with closest type strains obtained from the Ribosomal Database project in black font.

<p>Ant-derived contig IDs are given in red font. Red clade = <i>Firmicutes</i>, blue clade = <i>Bacteroidetes</i>, black clade = <i>Proteobacteria</i>, yellow clade = <i>Tenericutes</i>, green clade = <i>Actinobacteria.</i></p

16s rRNA sequence analysis.

<p>Phylum, subphylum and genus from the RDP Classifier. The closest GenBank match is given with the total expression value from both FIMM and BGI data, together with a short description of the most likely habitat, derived from GenBank accessions and literature (further details in the discussion).</p

Assembly metrics for <i>E.coli</i> genome assembled from Illumina paired end data with Velvet, SOAPdenovo, ABySS, Meraculous and IDBA-UD.

<p>The expected genome size for <i>E.coli</i> MG1655 is 4639675 bases.</p

Memory requirement for genome assembly.

<p>Memory required to assemble <i>E.coli</i> (<b>A</b>), <i>S.kudriavzevii</i> (<b>B</b>) and <i>C.elegans</i> (<b>C</b>) genomes increased, although not proportionately, with increasing depth of sequencing.</p

Assembly metrics for <i>S.kudriavzevii</i> genome assembled from Illumina paired end data with Velvet, SOAPdenovo, ABySS, Meraculous and IDBA-UD.

<p>The size of the assembled genome we used ZP_591 as reference is 11201698 bases.</p