Mercury accumulation in kidney lysosomes of proteinuric rats

Mercury accumulation in kidney lysosomes of proteinuric rats. The purpose of the present study was to determine whether lysosomal accumulation of mercury in the kidney is due to a leakage of protein-bound mercury through the glomerular filtration barrier followed by reabsorption into the lysosomal system of the proximal tubule. The subcellular distribution of mercury in the kidney was studied in four different groups of rats with and without proteinuria: normal young rats, young rats With amino-nucleoside nephrosis, old rats with spontaneous proteinuria, and old rats with chronic mercury intoxication and proteinuria. Radioactive mercuric chloride (203HgCl2) was injected s.c. into the rats 72 hours before sacrifice. Cell fractionation experiments were carried out on homogenates of the renal cortex by differential centrifugation. Determination of radioactive mercury in the subcellular fractions revealed that mercury was concentrated in the lysosomal fraction of all rats with proteinuria. In contrast, normal rats without proteinuria had the highest concentration of mercury in the supernatant, and there was no enrichment of mercury in the lysosomal fraction. Gel filtration chromatography performed on urine samples from proteinuric rats demonstrated that excreted mercury was associated with the albumin fraction. The accumulation of mercury in renal lysosomes of proteinuric rats and the demonstration of mercury bound to albumin in the urine support the hypothesis that mercury bound to plasma proteins passes the glomerular filtration barrier in proteinuric conditions and enters the lysosomal system of the proximal tubule by way of endocytosis.Accumulation de mercure dans les lysosomes rénaux de rats protéinuriques. Le but de ce travail a été de déterminer si l'accumulation lysosomale de mercure dans le rein est due à une fuite de mercure lié aux protéines à travers la barrière de filtration glomérulaire suivie d'une réabsorption dans le système lysosomal du tube proximal. La distribution subcellulaire du mercure a été étudiée dans quatre groupes de rats : des jeunes rats normaux, des jeunes rats atteints de néphropathie de l'aminonucléoside, des rats âgés avec une protéinurie spontanée et des rats âgés avec une intoxication mercurielle chronique et une proteinurie Du mercure radioactif (203HgCl2) a été injecté par voie sous-cutanée à des rats 72 heures avant le sacrifice. Le fractionnement cellulaire a été réalisé sur des homogénats de cortex rénal par centrifugation différentielle. La détermination du mercure radioactif dans les fractions subcellulaires a révélé que le mercure est concentré dans la fraction lysosomale de tous les rats protéinuriques. Les rats normaux sans protéinurie au contraire, ont une concentration de mercure plus élevée dans le surnageant et il n'y a pas d'enrichissement en mercure de la fraction lysosomale. La chromatographie par filtration sur gel réalisée sur les échantillons d'urine de rats protéinuriques a démontré que du mercure excrété est associée à la fraction albuminique. L'accumulation de mercure dans les lysosomes rénaux des rats protéinuriques et la mise en évidence de mercure lié à l'albumine dans l'urine sont en faveur de l'hypothèse selon laquelle le mercure lié à des protéines plasmatiques passe la barrière de filtration glomérulaire dans les situations où il existe une protéinurie et entre dans le système lysosomal du tube proximal par endocytose

Seniors Anti-bullying Project: Implementation Plan

Poster presented at the Canadian Association on Gerontology (CAG) Conference 202

Seniors Bullying in Ontario, The Sheridan Centre for Elder Research Preliminary Research Data

This webinar provides a project overview, demographic representation, preliminary statistics, and next steps of the Senior Bullying Project at Sheridan College in partnership with Elder Abuse Ontario and the Centre for Elder Research at Sheridan College

Senior Bullying: What is Happening in Ontario

Provide background and outline for the Ontario senior to senior prevalence study. This webinar also presents a definition of senior bullying and the need for a common understanding about what constitutes senior bullying. Webinar will also explain the process going forward – for example, survey distribution, accessing the website, languages available for the survey, print versions

Management of obesity in cats

Kirsten M Hoelmkjaer, Charlotte R Bjornvad Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark Abstract: Obesity is a common nutritional disorder in cats, especially when they are neutered and middle-aged. Obesity predisposes cats to several metabolic and clinical disorders, including insulin resistance, diabetes mellitus, lameness, and skin disease. Prevention and treatment of obesity is therefore of great importance in veterinary practice. Correct assessment of body composition is important for recognizing early states of obesity and for monitoring success of weight-loss programs. Various methods for assessing body composition have been proposed, of which a 9-point body-condition score has been validated in cats, and is possibly the most simple to use in the clinic; however, for extremely obese individuals, it is less useful. When calculating the appropriate daily caloric intake for a weight-loss plan, the aim is to maintain a safe weight-loss rate, increasing the chance of preserving lean body mass and decreasing the risk of developing hepatic lipidosis, while also producing a sufficient weight-loss rate to keep owners motivated. A weight-loss rate of 0.5%–2% per week is recommended, which for a cat that needs to lose 3 kg body weight results in an anticipated time for reaching the target weight of 24–60 weeks. There are several purpose-made weight-loss diets available. The optimal composition of a weight-loss diet for cats is unknown, but most of the available products have lower caloric density, an increased nutrient:energy ratio, and higher protein and fiber content. Regular follow-up visits allow the caloric intake to be adjusted based on progress, and possibly increase the chance of success. This review discusses the risk factors for and consequences of obesity, and gives directions for formulating a weight-loss plan, including daily caloric intake, choice of diet, and common problems based on the current literature. This review further provides a nutritional comparison of the current composition of selected commercial veterinary-specific weight-loss diets. Keywords: body condition, glucose intolerance, veterinary weight-loss diets, weight-loss managemen

Senior to Senior Bullying Project: Toolkit Launch

Official introduction to the Senior to Senior Anti-bullying Toolkit. The presentation shows how this toolkit was developed, how to use it, and when and how to access the document itself

Senior to Senior Anti-Bullying Toolkit

People of all ages can be bullied. The Centre for Elder Research worked with Sheridan Professor Dr. Kirsten Madsen and Elder Abuse Prevention (ON) to better understand bullying between older adults age 55+ in Ontario. Our project aims were to collect data on the prevalence and nature of Senior to Senior bullying in Ontario, and to use this data to create this Toolkit outlining best practices and strategies for older adults and those working with older adults to stop bullying. The Toolkit is part of a research project titled “Bullying between Older Adults: What is Happening in Ontario”? The research focused on aims to support the development of best practices and strategies for this diverse population of older adults.https://source.sheridancollege.ca/centres_elder_seniors_anti-bullying_toolkit/1001/thumbnail.jp