Sorption Properties of Polymeric Enterosorbent and Its Specific Modified Analogue in Simulated Cholera Toxin Solutions <I>in vitro</I>

Carried out was comparative study of the sorption properties in polymeric enterosorbent - chitosan, and its specific modified analogue obtained through absorption of anti-toxin immunoglobulins onto the soluble chitosan template. It is determined that soluble chitosan and its specific counterpart express high sorption activity in relation to cholera toxin, thus making lower its initial concentrations. Equilibrium adsorption capacity of the chitosan and its modified variant increase with the raise of initial cholera toxin concentration in the solution. Cholera toxin extraction rate was identified under static conditions. Adsorbate substance recovery rate was registered in the progression. In the course of experiments proved was modified sorbent specific sorption efficiency, occasioned by the presence of the active component in the analogue composition - namely, anti-toxic anticholeraic immunoglobulins. The data obtained are of the importance for further development of specific anticholeraic enterosorbent

Quantum analogue of the spin-flop transition for a spin pair

Quantum (step-like) magnetization curves are studies for a spin pair with antiferromagnetic coupling in the presence of a magnetic field parallel to the easy axis of the magnetic anisotropy. The consideration is done both analytically and numerically for a wide range of the anisotropy constants and spins up to $S \gtrsim 100$. Depending on the origin of the anisotropy (exchange or single-ion), the magnetization curve can demonstrate the jumps more than unity and the concentration of the unit jumps in a narrow range of the field. We also point the region of the problem parameters, where the behavior is quasiclassical for $S = 5$, and where system is substantially quantum in the limit $S \to \infty$.Comment: 5 pages, 5 figure

Prospective Sorption Matrices for Antitoxic Cholera Enterosorbent Constructing

Demonstrated is the possibility of constructing of oral anti-cholera preparations based on sorption matrices with immobilized specific ligand. Various enterosorbents (activated charcoal, polysorb, polyfepan, and chitosan) have been used as sorption matrices, cholera antitoxic immunoglobulins (ATIg) – as specific ligands. Toxin-neutralizing activity of the ATIg immobilized on polysorb has been observed in vivo in 1:64000 dilutions and that of ATIg immobilized on activated charcoal, polyfepan, and chitosan – in 1:32000. Their specific activity in vitro has been defined in dot-blot immunoassay in 1:10000 and 1:5000 dilutions, respectively. High toxin-neutralizing activity, as well as specific one has proved the suitability of all tested matrices for antitoxic cholera enterosorbents constructing. However, polysorb and chitosan are accepted to be the most prospective matrices in view of the studied characteristics and properties. To stabilize the properties of the obtained experimental prototype sorbents, AT/Ig-polysorb and AT/Ig-chitosan, applied has been the method of liophilisation with glycocol stabilizer. Dried samples retain their original biological properties

Chirality tunneling in mesoscopic antiferromagnetic domain walls

We consider a domain wall in the mesoscopic quasi-one-dimensional sample (wire or stripe) of weakly anisotropic two-sublattice antiferromagnet, and estimate the probability of tunneling between two domain wall states with different chirality. Topological effects forbid tunneling for the systems with half-integer spin S of magnetic atoms which consist of odd number of chains N. External magnetic field yields an additional contribution to the Berry phase, resulting in the appearance of two different tunnel splittings in any experimental setup involving a mixture of odd and even N, and in oscillating field dependence of the tunneling rate with the period proportional to 1/N.Comment: 4 pages + 2 figures, references correcte

Гендерное сравнение непосредственных результатов коронарных шунтирований на работающем сердце при остром инфаркте миокарда без подъема сегмента ST

Aim.To determine gender differences in immediate outcomes of patients with acute non-ST-elevation myocardial infarction undergoing off-pump coronary artery bypass grafting (CABG).Methods.CABG was indicated to patients with persistent myocardial ischemia (postinfarction angina) who did not meet criteria for percutaneous coronary intervention. Hybrid procedures and CABG aimed at repairing mechanical complications of AMI were not considered. Men (n = 66, 65.3%) were assigned to Group 1, whereas women (n = 35, 34.7%) were assigned to Group 2. Women were older and obese (grades 2-3), had higher GRACE scores, levels of cardiospecific troponin and higher rate of type 2 diabetes (p&lt;0.05). Men and women commonly had three-vessel CAD (3.0; 4.0). The mean SYNTAX score for men and women was 29.0 (24.0; 33.0) and 27.0 (24.0; 28.0), p&gt;0.05.Results.The median preoperative period was 4.0 days (3.8; 5.0) in male patients and 5.0 (4.0; 6.0) days in female patients (p&gt;0.05). The groups did not have any significant differences in technical aspects of CABG (p&gt;0.05). Mortality was 3.0% in men and 2.9% in women (p&gt;0.05). No deaths were recorded from the onset of AMI till the first 72 hours after CABG. Three patients who under CABG died after 72 hours from the onset of MI (p&gt;0.05). The total number of complications was 13 (19.7%) in men and 5 (14.3%) in women (p&gt;0.05). There were no cases of conversion to cardiopulmonary bypass.Conclusion.The immediate outcomes of delayed of-pump CABG in both, men and women with acute non-ST-segment myocardial infarction, were comparable in mortality and complications (p&gt;0.05). The immediate outcomes of CABG performed within 72 hours in both, men and women, were comparable in mortality as compared to surgeries performed after 72 hours from the MI onset. Obtained data demonstrated safety of early open-heart surgeries for acute non-ST-elevation myocardial infarction.Цель.Гендерная оценка непосредственных результатов коронарного шунтирования (КШ) на работающем сердце при остром инфаркте миокарда (ОИМ) без подъема сегмента ST.Материалы и методы.Ретроспективно проанализированы 101 случай КШ без искусственного кровообращения при остром инфаркте миокарда без подъема сегмента ST, выполненные в 2017–2019 гг. Показанием для КШ являлась сохраняющаяся ишемия миокарда в виде постинфарктной стенокардии при невозможности выполнить чрескожное коронарное вмешательство. Гибридные операции и КШ с коррекцией механических осложнений ОИМ не рассматривались. Пациенты разделены на две группы: в первую (n = 66) вошли пациенты мужского пола, во вторую (n = 35) – женского.Результаты.В группах мужчин и женщин медианы предоперационного периода составили 4,0 (3,8; 5,0) и 5,0 (4,0; 6,0) сут. (р&lt;0,05). В отношении технических аспектов КШ группы не имели значимых различий (р&gt;0,05). Летальность у мужчин – 3,0%, женщин – 2,9% (р&gt;0,05). При проведении КШ в первые 72 ч от начала ОИМ летальных исходов не зарегистрировано. Все три летальных случая отмечены после операций, выполненных спустя 72 ч от начала заболевания (р&gt;0,05). Общее количество осложнений – 13 (19,7%) и 5 (14,3%) у мужчин и женщин (р&gt;0,05).Заключение.Независимо от гендерной принадлежности непосредственные результаты отсроченных КШ на работающем сердце при ОИМ без подъема сегмента ST характеризовались статистически сопоставимыми летальностью и осложнениями. Независимо от пола пациента КШ в первые 72 ч от начала ОИМ имели статистически сопоставимую летальность в сравнении с операциями, проведенными через 72 ч от дебюта ОИМ, что указывает на возможность безопасных «открытых» реваскуляризаций миокарда в ранние сроки ОИМ без подъема сегмента ST

Properties of Neutral Charmed Mesons in Proton--Nucleus Interactions at 70 GeV

The results of treatment of data obtained in the SERP-E-184experiment "Investigation of mechanisms of the production of charmed particles in proton-nucleus interactions at 70 GeV and their decays" by irradiating the active target of the SVD-2 facility consisting of carbon, silicon, and lead plates, are presented. After separating a signal from the two-particle decay of neutral charmed mesons and estimating the cross section for charm production at a threshold energy {\sigma}(c\v{c})=7.1 \pm 2.4(stat.) \pm 1.4(syst.) \mub/nucleon, some properties of D mesons are investigated. These include the dependence of the cross section on the target mass number (its A dependence); the behavior of the differential cross sections d{\sigma}/dpt2 and d{\sigma}/dxF; and the dependence of the parameter {\alpha} on the kinematical variables xF, pt2, and plab. The experimental results in question are compared with predictions obtained on the basis of the FRITIOF7.02 code.Comment: 9 pages, 9 figures,3 table

Cultural Antigen in the Technology for Anti-Rabies Immunoglobulin Obtainment from Equine Blood Serum

week after immunization (specific activity is identified using neutralization reaction on the model of white mice and dot-blot immunoassay). This level of activity is sufficient for the fractioning of immune serum and extraction of anti-rabies immunoglobulin. Physicochemical and biological properties of the anti-rabies immunoglobulin, obtained with the help of cultural antigen technique, meet the requirements stated in the normative documentation on anti-rabies immunoglobulins extracted from equine blood serum. Specific activity level of experimental batches of anti-rabies immunoglobulin, obtained with the help of cultural technologies, corresponds to 242 and 214 IU/ml

DEVELOPMENT OF PHARMACEUTICAL DOSAGE FORM OF ANTI-CHOLERA IMMUNE-ENTEROSORBENT

Objective of the study is to experimentally substantiate the possibility of production of anti-cholera immune-enterosorbent in the pharmaceutical form of a pill.Materials and methods: experimental antitoxic immune-enterosorbent against cholera, stabilized using cool dehumidification. Residual moisture of enterosorbent lyophilizate was determined by means of Sartorius MA 150 moisture meter. Screen assay of the powders and granulates was performed applying the sieving of bulk material samples through screen set with sieve openings of various sizes. Specification of tap density was carried out with the help of SVM-10 unit. Granulation of specific enterosorbent was done using GPCG 2 LabSystem. The pills were manufactured in tablet press, MiniTabT. The hardness of the pallets was carried out by means of TBH 125 TD tester. The test for friability of the pills was performed in GTA 120.Results and conclusions. Utilization of cellulose microcrystalline and polyvinyl pyrolidone as pharmaceutical aids for granulation of lactose monohydrate is experimentally substantiated. It is established that the obtained granulate with passable values of technological parameters can serve as the basis for pharmaceutical dosage form of specific enterosorbent. Identified has been optimum mass of anti-cholera enterosorbent tablet cores. Demonstrated has been the possibility of Acryl-eze enteric coating application as protective shell of the pills. Studied has been specific activity of the pelleted enterosorbent in vitro, verified is its resistance capacity under conditions modeling gastrointestinal tract. In consequence of the performed trials and tests, technology for the production of antitoxic enterosorbent against cholera – enteric-coated tablet – has been developed. Long-term trials: the storage of the constructed preparation at 4–8°C within 24 months period (the observation time) – have revealed retention of enterosorbent activity, which testifies to its stability and defined the shelf life of specific tableted anti-cholera immune-enterosorbent