The glutamine synthetase gene family in Populus

<p>Abstract</p> <p>Background</p> <p>Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is a key enzyme in ammonium assimilation and metabolism of higher plants. The current work was undertaken to develop a more comprehensive understanding of molecular and biochemical features of <it>GS </it>gene family in poplar, and to characterize the developmental regulation of <it>GS </it>expression in various tissues and at various times during the poplar perennial growth.</p> <p>Results</p> <p>The <it>GS </it>gene family consists of 8 different genes exhibiting all structural and regulatory elements consistent with their roles as functional genes. Our results indicate that the family members are organized in 4 groups of duplicated genes, 3 of which code for cytosolic GS isoforms (GS1) and 1 which codes for the choroplastic GS isoform (GS2). Our analysis shows that <it>Populus trichocarpa </it>is the first plant species in which it was observed the complete <it>GS </it>family duplicated. Detailed expression analyses have revealed specific spatial and seasonal patterns of <it>GS </it>expression in poplar. These data provide insights into the metabolic function of GS isoforms in poplar and pave the way for future functional studies.</p> <p>Conclusions</p> <p>Our data suggest that <it>GS </it>duplicates could have been retained in order to increase the amount of enzyme in a particular cell type. This possibility could contribute to the homeostasis of nitrogen metabolism in functions associated to changes in glutamine-derived metabolic products. The presence of duplicated <it>GS </it>genes in poplar could also contribute to diversification of the enzymatic properties for a particular GS isoform through the assembly of GS polypeptides into homo oligomeric and/or hetero oligomeric holoenzymes in specific cell types.</p

The Binary Fraction of Stars in Dwarf Galaxies: The Cases of Draco and Ursa Minor

Measuring the frequency of binary stars in dwarf spheroidal galaxies (dSphs) requires data taken over long time intervals. We combine radial velocity measurements from five literature sources taken over the course of ~30 years to yield the largest multi-epoch kinematic sample for stars in the dSphs Draco and Ursa Minor. With this data set, we are able to implement an improved version of the Bayesian technique described in Spencer et al. to evaluate the binary fraction of red giant stars in these dwarf galaxies. Assuming Duquennoy & Mayor period and mass ratio distributions, the binary fractions in Draco and Ursa Minor are 0.50_(-0.06)^(+0.04) and 0.78_(-0.08)^(+0.09), respectively. We find that a normal mass ratio distribution is preferred over a flat distribution, and that log-normal period distributions centered on long periods µ_(log P > 3.5) are preferred over distributions centered on short ones. We reanalyzed the binary fractions in Leo II, Carina, Fornax, Sculptor, and Sextans, and find that there is <1% chance that binary fraction is a constant quantity across all seven dwarfs, unless the period distribution varies greatly. This indicates that the binary populations in Milky Way dSphs are not identical in regard to their binary fractions, period distributions, or both. We consider many different properties of the dwarfs (e.g., mass, radius, luminosity, etc.) and find that binary fraction might be larger in dwarfs that formed their stars quickly and/or have high velocity dispersions

Stellar kinematics of dwarf galaxies from multi-epoch spectroscopy: application to Triangulum II

We present new MMT/Hectochelle spectroscopic measurements for 257 stars observed along the line of sight to the ultra-faint dwarf galaxy Triangulum II. Combining with results from previous Keck/DEIMOS spectroscopy, we obtain a sample that includes 16 likely members of Triangulum II, with up to 10 independent redshift measurements per star. To this multi-epoch kinematic data set we apply methodology that we develop in order to infer binary orbital parameters from sparsely sampled radial velocity curves with as few as two epochs. For a previously-identified (spatially unresolved) binary system in Tri~II, we infer an orbital solution with period $296.0_{-3.3}^{+3.8} \rm~ days$ , semi-major axis $1.12^{+0.41}_{-0.24}\rm~AU$, and a systemic velocity $-380.0 \pm 1.7 \rm~km ~s^{-1}$ that we then use in the analysis of Tri~II's internal kinematics. Despite this improvement in the modeling of binary star systems, the current data remain insufficient to resolve the velocity dispersion of Triangulum II. We instead find a 95% confidence upper limit of $\sigma_{v} \lesssim 3.4 \rm ~km~s^{-1}$

Impaired decisional impulsivity in pathological videogamers

Abstract Background Pathological gaming is an emerging and poorly understood problem. Impulsivity is commonly impaired in disorders of behavioural and substance addiction, hence we sought to systematically investigate the different subtypes of decisional and motor impulsivity in a well-defined pathological gaming cohort. Methods Fifty-two pathological gaming subjects and age-, gender- and IQ-matched healthy volunteers were tested on decisional impulsivity (Information Sampling Task testing reflection impulsivity and delay discounting questionnaire testing impulsive choice), and motor impulsivity (Stop Signal Task testing motor response inhibition, and the premature responding task). We used stringent diagnostic criteria highlighting functional impairment. Results In the Information Sampling Task, pathological gaming participants sampled less evidence prior to making a decision and scored fewer points compared with healthy volunteers. Gaming severity was also negatively correlated with evidence gathered and positively correlated with sampling error and points acquired. In the delay discounting task, pathological gamers made more impulsive choices, preferring smaller immediate over larger delayed rewards. Pathological gamers made more premature responses related to comorbid nicotine use. Greater number of hours played also correlated with a Motivational Index. Greater frequency of role playing games was associated with impaired motor response inhibition and strategy games with faster Go reaction time. Conclusions We show that pathological gaming is associated with impaired decisional impulsivity with negative consequences in task performance. Decisional impulsivity may be a potential target in therapeutic management

Stellar Velocities in the Carina, Fornax, Sculptor and Sextans dSph Galaxies: Data from the Magellan/MMFS Survey

We present spectroscopic data for individual stars observed from 2004 March through 2008 August as part of our Michigan/MIKE Fiber System (MMFS) survey of four dwarf spheroidal (dSph) galaxies: Carina, Fornax, Sculptor and Sextans. Using MMFS at the Magellan/Clay Telescope at Las Campanas Observatory, we have acquired 8855 spectra from 7103 red giant candidates in these Galactic satellites. We list measurements of each star's line-of-sight velocity (median error +/- 2.1 km/s) and spectral line indices for iron and magnesium absorption features. We use globular cluster spectra to calibrate the indices onto standard [Fe/H] metallicity scales, but comparison of the resulting metallicities with published values suggests that the MMFS indices are best used as indicators of relative, not absolute metallicity. The empirical distributions of velocity and spectral indices also allow us to quantify the amount of contamination by foreground stars. In a companion paper we develop an algorithm that evaluates the membership probability for each star, showing that the present MMFS sample contains more than 5000 dSph members, including 774 Carina members, 2483 Fornax members, 1365 Sculptor members, and 441 Sextans members.Comment: Accepted for publication in The Astronomical Journal. Full data tables will be included in the electronic article, and are available at http://www.ast.cam.ac.uk/~walker/mmfsdata.htm

Crater 2::An Extremely Cold Dark Matter Halo

<p>supplementary data products, including all sky-subtracted spectra from individual targets, as well as random draws from posterior PDFs for model parameters (see enclosed README file)</p> <p> </p

Magellan/M2FS Spectroscopy of Tucana 2 and Grus 1

We present results from spectroscopic observations with the Michigan/Magellan Fiber System (M2FS) of $147$ stellar targets along the line of sight to the newly-discovered `ultrafaint' stellar systems Tucana 2 (Tuc 2) and Grus 1 (Gru 1). Based on simultaneous estimates of line-of-sight velocity and stellar-atmospheric parameters, we identify 8 and 7 stars as probable members of Tuc 2 and and Gru 1, respectively. Our sample for Tuc 2 is sufficient to resolve an internal velocity dispersion of $8.6_{-2.7}^{+4.4}$ km s$^{-1}$ about a mean of $-129.1_{-3.5}^{+3.5}$ km s$^{-1}$ (solar rest frame), and to estimate a mean metallicity of [Fe/H]= $-2.23_{-0.12}^{+0.18}$. These results place Tuc 2 on chemodynamical scaling relations followed by dwarf galaxies, suggesting a dominant dark matter component with dynamical mass $2.7_{-1.3}^{+3.1}\times 10^6$ $\mathrm{M}_{\odot}$ enclosed within the central $\sim 160$ pc, and dynamical mass-to-light ratio $1900_{-900}^{+2200}$ $\mathrm{M}_{\odot}/L_{V,\odot}$. For Gru 1 we estimate a mean velocity of $-140.5_{-1.6}^{+2.4}$ km s$^{-1}$ and a mean metallicity of [Fe/H]=$-1.42_{-0.42}^{+0.55}$, but our sample does not resolve Gru 1's velocity dispersion. The radial coordinates of Tuc 2 and Gru 1 in Galactic phase space suggest that their orbits are among the most energetic within distance $\leq 300$ kpc. Moreover, their proximity to each other in this space arises naturally if both objects are trailing the Large Magellanic Cloud.Comment: replaced with ApJ-accepted version, all spectra and data products (including samples from posterior PDFs) are available at http://www.andrew.cmu.edu/user/mgwalker/tuc2gru1_dataproducts.tar.g

Neuroinvasion and Neurotropism by SARS-CoV-2 Variants in the K18-hACE2 Mouse

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) not only affects the respiratory tract but also causes neurological symptoms such as loss of smell and taste, headache, fatigue or severe cerebrovascular complications. Using transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2), we investigated the spatiotemporal distribution and pathomorphological features in the CNS following intranasal infection with SARS-CoV-2 variants, as well as after prior influenza A virus infection. Apart from Omicron, we found all variants to frequently spread to and within the CNS. Infection was restricted to neurons and appeared to spread from the olfactory bulb mainly in basally oriented regions in the brain and into the spinal cord, independent of ACE2 expression and without evidence of neuronal cell death, axonal damage or demyelination. However, microglial activation, microgliosis and a mild macrophage and T cell dominated inflammatory response was consistently observed, accompanied by apoptotic death of endothelial, microglial and immune cells, without their apparent infection. Microgliosis and immune cell apoptosis indicate a potential role of microglia for pathogenesis and viral effect in COVID-19 and the possible impairment of neurological functions, especially in long COVID. These data may also be informative for the selection of therapeutic candidates and broadly support the investigation of agents with adequate penetration into relevant regions of the CNS

The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe

The preponderance of matter over antimatter in the early Universe, the dynamics of the supernova bursts that produced the heavy elements necessary for life and whether protons eventually decay --- these mysteries at the forefront of particle physics and astrophysics are key to understanding the early evolution of our Universe, its current state and its eventual fate. The Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed plan for a world-class experiment dedicated to addressing these questions. LBNE is conceived around three central components: (1) a new, high-intensity neutrino source generated from a megawatt-class proton accelerator at Fermi National Accelerator Laboratory, (2) a near neutrino detector just downstream of the source, and (3) a massive liquid argon time-projection chamber deployed as a far detector deep underground at the Sanford Underground Research Facility. This facility, located at the site of the former Homestake Mine in Lead, South Dakota, is approximately 1,300 km from the neutrino source at Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino charge-parity symmetry violation and mass ordering effects. This ambitious yet cost-effective design incorporates scalability and flexibility and can accommodate a variety of upgrades and contributions. With its exceptional combination of experimental configuration, technical capabilities, and potential for transformative discoveries, LBNE promises to be a vital facility for the field of particle physics worldwide, providing physicists from around the globe with opportunities to collaborate in a twenty to thirty year program of exciting science. In this document we provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess.Comment: Major update of previous version. This is the reference document for LBNE science program and current status. Chapters 1, 3, and 9 provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess. 288 pages, 116 figure