19 research outputs found

    GLUTATHIONE S-TRANSFERASE PLACENTAL FORM IS A MARKER FOR BILE DUCT CARCINOMA, BUT NOT HEPATOCELLULAR CARCINOMA, IN HUMANS.

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    Immunohistochemical studies using anti-human glutathione S-transferase placental form (GST-π) rabbit antibody were carried out to investigate various hepatobiliary diseases in humans. Hepatocytes in fetal and adult liver without disease were negatively or positively stained while intra-or extrahepatic bile duct epithelial cells were positively or strongly positively stained with GST-π. Hepatocytes in regenerated nodules in liver cirrhosis were positively stained. Hepatocellular carcinomas were not strongly positively stained, while cholangiocarcinomas and cancers of the biliary tract were positively or strongly positively stained. These results indicate that GST-π staining is a useful marker for the diagnosis of intra-or extra-hepatic bile duct carcinomas in humans, and that enzyme activity is not phenotypically expressed in hepatocellular carcinomas

    Effects of valproic acid on the cell cycle and apoptosis through acetylation of histone and tubulin in a scirrhous gastric cancer cell line

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    <p>Abstract</p> <p>Background</p> <p>Management of peritoneal dissemination is the most critical problem in gastric cancer. This study was performed to investigate the inhibitory effects of valproic acid (VPA) on a highly peritoneal-seeding cell line of human scirrhous gastric cancer, OCUM-2MD3, and to explore the mechanism and the potential of VPA.</p> <p>Methods</p> <p>The effects of VPA on the growth of OCUM-2MD3 cells were assessed by MTT assay. In addition, paclitaxel (PTX) was combined with VPA to evaluate their synergistic effects. HDAC1 and HDAC2 expression were evaluated by western blotting in OCUM-2MD3 cells and other gastric cancer cell lines (TMK-1, MKN-28). The acetylation status of histone H3 and α-tubulin after exposure to VPA were analyzed by western blotting. The activities of cell cycle regulatory proteins and apoptosis-modulating proteins were also examined by western blotting. The effects of VPA <it>in vivo </it>were evaluated in a xenograft model, and apoptotic activity was assessed by TUNEL assay.</p> <p>Results</p> <p>OCUM-2MD3 cells showed high levels of HDAC1 and HDAC2 expression compared with TMK-1 and MKN-28. The concentration of VPA required for significant inhibition of cell viability (<it>P </it>< 0.05) was 5 mM at 24 h and 0.5 mM at 48 h and 72 h. The inhibition of VPA with PTX showed dose-dependent and combinatorial effects. VPA increased acetyl-histone H3, acetyl-α-tubulin, and p21WAF1 levels accompanied by upregulation of p27, caspase 3, and caspase 9, and downregulation of bcl-2, cyclin D1, and survivin. In the xenograft model experiment, the mean tumor volume of the VPA-treated group was significantly reduced by 36.4%, compared with that of the control group at 4 weeks after treatment (<it>P </it>< 0.01). The apoptotic index was significantly higher in the VPA-treated group (42.3% ± 3.5%) than in the control group (7.7% ± 2.5%) (<it>P </it>< 0.001).</p> <p>Conclusions</p> <p>VPA induced dynamic modulation of histone H3 and α-tubulin acetylation in relation with the anticancer effect and the enhancement of PTX in the OCUM-2MD3 cell line. Therefore, VPA in combination with PTX is expected to be a promising therapy for peritoneal dissemination of scirrhous gastric cancer.</p

    The Evolution of Vigorous Protests against the Revision and Renewal of U.S.Japanese Security Treaty in Fukuoka Prefecture

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    はじめに, 1.福岡県安保共闘会議の結成, (1).結成大会, (2).結成までの経緯, 2.安保共闘の組織化過程, (1).地区共闘の結成, (2).校区・町村共闘の結成, (3).職場での安保共闘の組織化, 3.福岡県下の運動展開の特色, (1).「最高限実力行使決議」の集約, (2).九州拠点の統一行動とのかかわり, (3).批准反対請願署名運動の展開, (4).三池闘争とのかかわり, (5).六・四・六・一五の戦い, 4.県安保共闘の運動総括について, (1).第一九回拡大幹事会での総括, (2).県安保共闘の性格 - カンパニア共闘の壁, (3).県安保共闘の財政問題, おわり

    Voting Behaviour in Kitakyushu City

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    はじめに, 1.北九州市の社会的特色, 2.総選挙での投票行動, 3.参院選挙(地方区)の投票行動, 4.県議選での投票行動, 5.市議選での投票行動, むすびにかえ

    北九州市民の投票行動

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    はじめに, 1.北九州市の社会的特色, 2.総選挙での投票行動, 3.参院選挙(地方区)の投票行動, 4.県議選での投票行動, 5.市議選での投票行動, むすびにかえ

    六〇年安保闘争の福岡県での展開 : 県安保共闘の組織化過程を中心として

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    はじめに, 1.福岡県安保共闘会議の結成, (1).結成大会, (2).結成までの経緯, 2.安保共闘の組織化過程, (1).地区共闘の結成, (2).校区・町村共闘の結成, (3).職場での安保共闘の組織化, 3.福岡県下の運動展開の特色, (1).「最高限実力行使決議」の集約, (2).九州拠点の統一行動とのかかわり, (3).批准反対請願署名運動の展開, (4).三池闘争とのかかわり, (5).六・四・六・一五の戦い, 4.県安保共闘の運動総括について, (1).第一九回拡大幹事会での総括, (2).県安保共闘の性格 - カンパニア共闘の壁, (3).県安保共闘の財政問題, おわり

    Body Mass Index Modified Predictive Ability Of Biomarkers For Protein-Energy Wasting In Prevalent Hemodialysis Patients

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    Protein-energy wasting (PEW) is crucial complication in prevalent hemodialysis (HD) patients. Abnormality of biomarkers such as IL-6, NT-pro-BNP, and adiponectin (ADPN) contribute to PEW. However, associations of the biomarkers with PEW according to BMI levels have not been fully evaluated. Thus those associations were assessed by a cross-sectional cohort study of prevalent HD patients (n=386). PEW was defined by subjective global assessment (n=108). IL-6 levels were inversely, and NT-pro-BNP and ADPN levels were positively correlated with BMI levels. Odds ratio (OR) of log IL-6 (OR 1.5, CI 1.0, 2.2), log NT-pro-BNP (OR 1.7, CI 1.3, 2.2), and log ADPN (OR 1.9, CI 1.1, 3.2) to PEW were significantly increased in logistic multivariate regression model. ORs of log NT-pro-BNP and log ADPN for PEW were progressively increased in patients with threshold 21 kg/m2<BMI, but progressively decreased in those below this threshold. On the other hand, OR of log IL-6 for PEW was progressively decreased in patients with threshold 21 kg/m2<BMI, but similar in those below this threshold. These associations were confirmed by the models adjusted with confounder. In conclusions, predictive values of IL-6, NT-pro-BNP and ADPN for PEW state may be dependent on BMI levels in prevalent HD patients.fx
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